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Genetic Insert: FRB-HA (no pA)
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pDONR P2R-P3; Vector Types:; Bacterial Resistance:Kanamycin
Defining Citation: PMID:27500400
Proper citation: RRID:Addgene_82597 Copy
Genetic Insert: FKBP-HA (no pA)
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pDONR P2R-P3; Vector Types:; Bacterial Resistance:Kanamycin
Defining Citation: PMID:27500400
Proper citation: RRID:Addgene_82598 Copy
Genetic Insert: SBP-TEV-2x protein G
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pDONR P2R-P3; Vector Types:; Bacterial Resistance:Kanamycin
Defining Citation: PMID:27500400
Proper citation: RRID:Addgene_82599 Copy
Genetic Insert: FRB-HA (no pA)
Vector Backbone Description: Vector Backbone:pDONR P2R-P3; Vector Types:; Bacterial Resistance:Kanamycin
Defining Citation: PMID:27500400
Proper citation: RRID:Addgene_82594 Copy
Genetic Insert: human ubiquitin C promoter followed by intronically expressed eSIBR cassette
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pDONR P4-P1R; Vector Types:; Bacterial Resistance:Kanamycin
Defining Citation: PMID:27500400
Proper citation: RRID:Addgene_82586 Copy
Genetic Insert: 395 sgRNAs targeting mouse mitochondrial DNA
Vector Backbone Description: Vector Backbone:pUC57kan-T7-gRNA; Vector Types:; Bacterial Resistance:Kanamycin
Defining Citation: PMID:27309802
Proper citation: RRID:Addgene_82480 Copy
Species: Pseudomonas putida KT2440
Genetic Insert: PP3359
Vector Backbone Description: Backbone Marker:Jay Keasling's lab; Backbone Size:3281; Vector Backbone:pBbE8k; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:27559924
Proper citation: RRID:Addgene_83067 Copy
Species: Homo sapiens
Genetic Insert: Ubiqutin
Vector Backbone Description: Backbone Size:5289; Vector Backbone:pHIS parallele 2; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:27694912
Proper citation: RRID:Addgene_83234 Copy
Species: Homo sapiens
Genetic Insert: Tyrosinase
Vector Backbone Description: Backbone Size:4904; Vector Backbone:piRFP682; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:27140606
Proper citation: RRID:Addgene_80152 Copy
Species: Synthetic
Genetic Insert: AAVS1 TALEN (Left)
Vector Backbone Description: Backbone Size:2300; Vector Backbone:pDONR221; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:26707206
Comments: Gateway assembled into a custom Gateway Entry vector using Addgene Kit #1000000024
Proper citation: RRID:Addgene_80495 Copy
Genetic Insert: none
Vector Backbone Description: Vector Backbone:none; Vector Types:; Bacterial Resistance:Kanamycin
Defining Citation: PMID:27562138
Comments: Genotype: ΔmetE Tn7::PcpcG2Δ59-metE
Precursor strain: JT2 (Tabor JJ, Levskaya A, and Voigt CA. 2011. J Mol Biol, 405(2): 315-324.) Endogenous metE has been deleted and metE under the cpcG2Δ59 promoter has been integrated into the Tn7 attachment site on the chromosome.
metE was deleted from strain JT2 using λ-Red recombination (Baba T, et al. 2006. Mol Syst Biol, 2. doi: 10.1038/msb4100050). PcpcG2Δ59-metE was then integrated into the Tn7 attachment site by tri-parental mating with Addgene plasmid pSKA397 (Plasmid #80380), helper plasmid pTNS3 (Choi KH, et al. 2008. Applied and Environmental Microbiology, 74(4): 1064-1075.), and E. coli mating strain MFDpir (Ferrières L, et al. 2010. Journal of Bacteriology, 192(24): 6418-6427.).
Proper citation: RRID:Addgene_80403 Copy
Genetic Insert: ffGFP-3xFLAG
Vector Backbone Description: Backbone Size:6100; Vector Backbone:pBBR1; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:24847683
Proper citation: RRID:Addgene_80400 Copy
Vector Backbone Description: Backbone Marker:Lucigen; Vector Backbone:PSMARTHCKan; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:27499201
Comments: Insert complementary annealed oligonucleotides encoding desired crRNA into BbsI sites. Include following overhangs:
5' ODN: CACCG(N19)
3' ODN: AAAC(N19)C
Proper citation: RRID:Addgene_80427 Copy
Species: Synthetic
Genetic Insert: EPN-01*
Vector Backbone Description: Backbone Marker:EMD Biosciences; Vector Backbone:pET29b; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:27919066
Proper citation: RRID:Addgene_80423 Copy
Species: Synechococcus elongatus PCC 7942
Genetic Insert: Sucrose phosphate synthase
Vector Backbone Description: Vector Backbone:NS2; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:27742883
Proper citation: RRID:Addgene_80459 Copy
Genetic Insert: GFPcaax no pA p3E
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2619; Vector Backbone:pDONR P2R-P3; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:27500400
Proper citation: RRID:Addgene_80817 Copy
Species: Other
Genetic Insert: GFP no pA
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2619; Vector Backbone:pDONR P2R-P3; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:27500400
Proper citation: RRID:Addgene_80813 Copy
Vector Backbone Description: Backbone Marker:Wang, R.F. and S. R. Kushner. 1991. Gene 100:195-199.; Vector Backbone:pWSK29; Vector Types:; Bacterial Resistance:Kanamycin
Defining Citation: PMID:9887310
Proper citation: RRID:Addgene_80779 Copy
Genetic Insert: GFP
Vector Backbone Description: Backbone Marker:N/A; Vector Backbone:N/A; Vector Types:p15A origin, constitutive promoter, KanR; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25126893
Proper citation: RRID:Addgene_80652 Copy
Species: Other
Genetic Insert: pME-mKate2 no stop
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2518; Vector Backbone:pDONR221; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:27500400
Proper citation: RRID:Addgene_80806 Copy
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