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Species: Homo sapiens
Genetic Insert: Bik, Bcl-2-interacting killer, or Apoptosis inducer NBK
Vector Backbone Description: Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Comments: Transfection of this plasmid will express Venus-fused to the N-terminus of Bik-GG153AA. There is a flexible, 8 aa linker "SGLRSRGG", between Venus and Bik-GG153AA. This Venus protein contains an F224R mutation to make it monomeric.
Proper citation: RRID:Addgene_177427 Copy
Species: Pseudomonas aeruginosa
Genetic Insert: sptse5-CT (1169-1269)
Vector Backbone Description: Backbone Marker:Ladant Lab (https://research.pasteur.fr/en/team/biochemistry-of-macromolecular-interactions/); Vector Backbone:pKTop; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:36335275
Comments: Please visit https://doi.org/10.21203/rs.3.rs-967174/v1 for preprint.
Proper citation: RRID:Addgene_192949 Copy
Species: Homo sapiens
Genetic Insert: Bcl-2-Cb5, Bcl2-cb5, Bcl-cb5
Vector Backbone Description: Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Comments: Transfection of this plasmid will express Venus-fused to the N-terminus of Bcl-2-Cb5. There is a flexible, 5 aa linker "SGLRS", between Venus and Bcl2-Cb5. This Venus protein contains an F224R mutation to make it monomeric.
Proper citation: RRID:Addgene_177422 Copy
Species: Homo sapiens
Genetic Insert: Bcl-2-Cb5, Bcl2-cb5, Bcl-cb5
Vector Backbone Description: Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Comments: Transfection of this plasmid will express mCerulean3-fused to the N-terminus of Bcl-2-Cb5. There is a flexible, 7 aa linker "SGLRSTR", between mCerulean3 and Bcl-2-Cb5.
Proper citation: RRID:Addgene_177421 Copy
Species: Homo sapiens
Genetic Insert: Bik, Bcl-2-interacting killer, or Apoptosis inducer NBK
Vector Backbone Description: Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Comments: Transfection of this plasmid will express Venus-fused to the N-terminus of Bik-L61G. There is a flexible, 8 aa linker "SGLRSRGG", between Venus and Bik-L61G. This Venus protein contains an F224R mutation to make it monomeric.
Proper citation: RRID:Addgene_177426 Copy
Species: Pseudomonas aeruginosa
Genetic Insert: sptse5-CT (1169-1300)
Vector Backbone Description: Backbone Marker:Ladant Lab (https://research.pasteur.fr/en/team/biochemistry-of-macromolecular-interactions/); Vector Backbone:pKTop; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:36335275
Comments: Please visit https://doi.org/10.21203/rs.3.rs-967174/v1 for preprint.
Proper citation: RRID:Addgene_192951 Copy
Species: Pseudomonas aeruginosa
Genetic Insert: sptse5-CT (1169-1281)
Vector Backbone Description: Backbone Marker:Ladant Lab (https://research.pasteur.fr/en/team/biochemistry-of-macromolecular-interactions/); Vector Backbone:pKTop; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:36335275
Comments: Please visit https://doi.org/10.21203/rs.3.rs-967174/v1 for preprint.
Proper citation: RRID:Addgene_192950 Copy
Species: Pseudomonas aeruginosa
Genetic Insert: tse5-CT (1169-1229)
Vector Backbone Description: Backbone Marker:Ladant Lab (https://research.pasteur.fr/en/team/biochemistry-of-macromolecular-interactions/); Vector Backbone:pKTop; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:36335275
Comments: Please visit https://doi.org/10.21203/rs.3.rs-967174/v1 for preprint.
Proper citation: RRID:Addgene_192953 Copy
Species: Pseudomonas aeruginosa
Genetic Insert: sptse5-CT
Vector Backbone Description: Backbone Marker:de Lorenzo Lab (https://vdl-lab.com/); Vector Backbone:pS238D1; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:36335275
Comments: Please visit https://doi.org/10.21203/rs.3.rs-967174/v1 for preprint.
Proper citation: RRID:Addgene_192959 Copy
Species: Pseudomonas aeruginosa
Genetic Insert: tse5-CT (1169-1317)
Vector Backbone Description: Backbone Marker:Ladant Lab (https://research.pasteur.fr/en/team/biochemistry-of-macromolecular-interactions/); Vector Backbone:pKTop; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:36335275
Comments: Please visit https://doi.org/10.21203/rs.3.rs-967174/v1 for preprint.
Proper citation: RRID:Addgene_192957 Copy
Species: Homo sapiens
Genetic Insert: Bcl-2-ActA, Bcl2-acta, Bcl-acta
Vector Backbone Description: Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Comments: Transfection of this plasmid will express Venus-fused to the N-terminus of Bcl-2-ActA. There is a flexible, 6 amino acid linker "SGLRST", between Venus and Bcl-2-ActA.
Proper citation: RRID:Addgene_177419 Copy
Species: Pseudomonas aeruginosa
Genetic Insert: tse5-CT (1169-1300)
Vector Backbone Description: Backbone Marker:Ladant Lab (https://research.pasteur.fr/en/team/biochemistry-of-macromolecular-interactions/); Vector Backbone:pKTop; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:36335275
Comments: Please visit https://doi.org/10.21203/rs.3.rs-967174/v1 for preprint.
Proper citation: RRID:Addgene_192956 Copy
Species: Homo sapiens
Genetic Insert: Bcl-XL-ActA, BclX-acta
Vector Backbone Description: Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Comments: Transfection of this plasmid will express Venus-fused to the N-terminus of Bcl-XL-ActA. There is a flexible, 7 aa linker "SGLRSRE", between Venus and BclXL-ActA. This Venus protein contains an F224R mutation to make it monomeric. Note that following the stop codon at the end of the “ActA” sequence, this plasmid contains a ~1000bp insertion in the original pEGFP-C1 backbone that consists partially of duplication of the backbone itself and a sequence left over from cut and paste from another backbone. Expression of the protein of interest, Venus-BclXL-ActA, in BMK and MCF-7 cells was not affected by this insertion.
Proper citation: RRID:Addgene_177410 Copy
Species: Homo sapiens
Genetic Insert: Bcl-XL-ActA, BclX-acta
Vector Backbone Description: Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Comments: Transfection of this plasmid will express mCerulean3-fused to the N-terminus of Bcl-XL-ActA. There is a flexible, 7 aa linker "SGLRSTR", between mCerulean3 and Bcl-XL-ActA.
Proper citation: RRID:Addgene_177412 Copy
Species: Homo sapiens
Genetic Insert: Bcl-XL-Cb5, BclX-cb5
Vector Backbone Description: Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Comments: Transfection of this plasmid will express Venus-fused to the N-terminus of Bcl-XL-Cb5. There is a flexible, 7 aa linker "SGLRSTR", between mCerulean3 and BclXL-Cb5.
Proper citation: RRID:Addgene_177415 Copy
Species: lambda phage, streptococcus pyogenes
Genetic Insert: Cas9, bet, exo
Vector Backbone Description: Vector Backbone:RSF1010; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:35701417
Comments: Please visit https://arxiv.org/pdf/2111.11880.pdf for preprint.
Proper citation: RRID:Addgene_184862 Copy
Species: E. coli, lambda phage
Genetic Insert: lacZalpha,ccdB
Vector Backbone Description: Vector Backbone:Entry vector; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:35701417
Comments: Please visit https://arxiv.org/pdf/2111.11880.pdf for preprint.
Proper citation: RRID:Addgene_184867 Copy
Species: E. coli, lambda phage
Genetic Insert: lacZalpha,ccdB
Vector Backbone Description: Vector Backbone:Entry vector; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:35701417
Comments: Please visit https://arxiv.org/pdf/2111.11880.pdf for preprint.
Proper citation: RRID:Addgene_184865 Copy
Species: Homo sapiens
Genetic Insert: Bik, Bcl-2-interacting killer, or Apoptosis inducer NBK
Vector Backbone Description: Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Comments: Transfection of this plasmid will express Venus-fused to the N-terminus of Bik-AA. There is a flexible, 8 aa linker "SGLRSRGG", between Venus and Bik-AA. This Venus protein contains an F224R mutation to make it monomeric.
Proper citation: RRID:Addgene_177430 Copy
Species: Homo sapiens
Genetic Insert: Bcl-2-interacting killer
Vector Backbone Description: Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_191411 Copy
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