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Species: Other
Genetic Insert: EGFP
Vector Backbone Description: Backbone Marker:Alexeyev lab; Backbone Size:4321; Vector Backbone:pMA898; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27136098
Proper citation: RRID:Addgene_70109 Copy
Species: Other
Genetic Insert: Luciferase
Vector Backbone Description: Vector Backbone:pPY.CAG.CRE.IP; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28341702
Proper citation: RRID:Addgene_74409 Copy
Species: Other
Genetic Insert: mPlum fluorescent protein
Vector Backbone Description: Backbone Marker:Aldevron; Backbone Size:5100; Vector Backbone:gWiz-blank; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Comments: Plum is a far red fluorescent protein.
Excitation 590 nm
Emission 649 nm
Proper citation: RRID:Addgene_74475 Copy
Species: Other
Genetic Insert: DD-SpCas9
Vector Backbone Description: Vector Backbone:modified pX330; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26762978
Comments: DD domain tested for stabilization with 500 nM Shield-1
Proper citation: RRID:Addgene_75380 Copy
Species: Other
Genetic Insert: Cm(R)-CcdB
Vector Backbone Description: Backbone Size:2000; Vector Backbone:pUC19; Vector Types:CRISPR; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:27088723
Comments: Want all of the CRISPRainbow constructs? Request the set here: https://www.addgene.org/kits/pederson-crisprainbow/
Proper citation: RRID:Addgene_75398 Copy
Species: Other
Genetic Insert: EGFP-AID
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5161; Vector Backbone:pcDNA5-FRT-TO; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_80075 Copy
Species: Other
Genetic Insert: TREtight driven tdTomato fluorescent protein
Vector Backbone Description: Backbone Marker:Liqun Luo; Backbone Size:2562; Vector Backbone:pUAS-luc2; Vector Types:Mutli-species; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23953120
Comments: Tang J.C. et al. (2013). A nanobody-based system using fluorescent proteins as scaffolds for cell-specific gene manipulation. Cell. 2013 Aug 15;154(4):928-39.
Proper citation: RRID:Addgene_50798 Copy
Species: Other
Genetic Insert: Fok1 fused to dCas9
Vector Backbone Description: Backbone Size:3400; Vector Backbone:JDS246; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24770324
Proper citation: RRID:Addgene_52970 Copy
Species: Other
Genetic Insert: 2x membrane linker
Vector Backbone Description: Backbone Marker:RZPD; Backbone Size:4095; Vector Backbone:pCS2+; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19378112
Comments: Cut with NotI and transcribe with SP6 for RNA.
CDS of mCherry was PCR amplified to add AgeI at N terminus and SnaBI at C-terminus. Put
this fragment in AgeI/SnaBI cut pCS-memb-cerulean (Addgene plasmid 53749).
Proper citation: RRID:Addgene_53750 Copy
Species: Other
Genetic Insert: Rab5a
Vector Backbone Description: Backbone Size:4750; Vector Backbone:mApple; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Comments: . Excitation = 568; Emission = 592
Proper citation: RRID:Addgene_54944 Copy
Species: Other
Genetic Insert: BaxAlpha
Vector Backbone Description: Backbone Size:4750; Vector Backbone:mCherry-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Comments: . Excitation = 587; Emission = 610
Proper citation: RRID:Addgene_55000 Copy
Species: Other
Genetic Insert: VSVG
Vector Backbone Description: Backbone Size:4750; Vector Backbone:mCerulean; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Comments: . Excitation = 433; Emission = 475
Proper citation: RRID:Addgene_55397 Copy
Species: Other
Genetic Insert: Rab5a
Vector Backbone Description: Backbone Size:4750; Vector Backbone:mCerulean3; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:21479270
Comments: . Excitation = 433; Emission = 475
Proper citation: RRID:Addgene_55444 Copy
Species: Other
Genetic Insert: VSVG
Vector Backbone Description: Backbone Size:4750; Vector Backbone:mTFP1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:18325109
Comments: deltaK VSVG ts045. Excitation = 462; Emission = 492
Addgene sequencing result finds L394S, F440ins, and K511N in M11048.1.
Proper citation: RRID:Addgene_55517 Copy
Species: Other
Genetic Insert: SV40 T-Antigen Nuclear Localization Signal (NLS)
Vector Backbone Description: Backbone Size:4750; Vector Backbone:Kaede-NLS; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Comments: . Excitation = 508 / 572; Emission = 518 / 580
Proper citation: RRID:Addgene_57319 Copy
Species: Other
Genetic Insert: Rab5a
Vector Backbone Description: Backbone Size:4750; Vector Backbone:tdTomato; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Comments: . Excitation = 554; Emission = 581
Proper citation: RRID:Addgene_58126 Copy
Species: Other
Genetic Insert: neo
Vector Backbone Description: Vector Backbone:pCEP4; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25002522
Proper citation: RRID:Addgene_58527 Copy
Species: Other
Genetic Insert: firefly Luciferase
Vector Backbone Description: Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24503441
Proper citation: RRID:Addgene_58792 Copy
Species: Other
Genetic Insert: d2-EGFP
Vector Backbone Description: Vector Backbone:pUC; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:19561591
Proper citation: RRID:Addgene_59763 Copy
Species: Other
Genetic Insert: Luc-CTE
Vector Backbone Description: Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22253824
Proper citation: RRID:Addgene_60881 Copy
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