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Genetic Insert: GluN2A
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pLenti7.3; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25887710
Proper citation: RRID:Addgene_164724 Copy
Vector Backbone Description: Backbone Size:0; Vector Backbone:pSAR-MT; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:8755583
Comments: The pSAR-MT inducible
expression plasmid was constructed as follows. Two
oligonucleotides (5'-GATCTCGAGCTCCCTGCA-3' and 5'-GGGAGCTCGA-3') were annealed, and the resulting fragment was cloned into the BamHI and PstI sites of the scaffold attachment region (SAR)-containing plasmid p8. The resulting plasmid, pJM7, consisted of two SAR sequences
flanking a short polylinker containing restriction sites for Xhol and PstI. The mutant metallothionein (MT) promoter was amplified by PCR and cloned into pCEP4, linearized
with BglII and NotI, yielding pCEP-MT. A SalI-BamHI
fragment containing an intron from the globin gene was then
inserted into the XhoI and BamHI sites of pCEP-MT, yielding pCEP-MTi. Finally, the MT promoter/intron/poly(A) region was removed from pCEP-MTi by digestion with Sall and cloned into the XhoI site of pJM7, yielding pSAR-MT.
Proper citation: RRID:Addgene_16485 Copy
Species: Leptotrichia shahii
Genetic Insert: LsCas13a-gRNA-3
Vector Backbone Description: Backbone Size:3267; Vector Backbone:pBAD18; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34694607
Comments: Spacer sequence is designed to target the mRNA of deGFP.
Proper citation: RRID:Addgene_164867 Copy
Species: Leptotrichia shahii
Genetic Insert: LsCas13a-gRNA-2
Vector Backbone Description: Backbone Size:3267; Vector Backbone:pBAD18; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34694607
Comments: Spacer sequence is designed to target the mRNA of deGFP.
Proper citation: RRID:Addgene_164866 Copy
Genetic Insert: GluN2A
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pLenti7.3DEST; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25887710
Proper citation: RRID:Addgene_164776 Copy
Species: Mus musculus
Genetic Insert: nAChR b2 E61C - IRES- GFP
Vector Backbone Description: Backbone Size:9424; Vector Backbone:pLenti; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30176987
Proper citation: RRID:Addgene_164777 Copy
Species: Mus musculus
Genetic Insert: p21
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:3000; Vector Backbone:pBluescript SK; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:7796420
Proper citation: RRID:Addgene_16478 Copy
Vector Backbone Description: Vector Backbone:pX330; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33619540
Proper citation: RRID:Addgene_164802 Copy
Genetic Insert: ERH CRISPRi gRNA1
Vector Backbone Description: Backbone Marker:Jonathan Weissman (Addgene plasmid # 60955); Vector Backbone:pU6-sgRNA EF1Alpha-puro-T2A-BFP; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32302541
Proper citation: RRID:Addgene_164800 Copy
Vector Backbone Description: Vector Backbone:pX330; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33619540
Proper citation: RRID:Addgene_164804 Copy
Species: Homo sapiens
Genetic Insert: miR-144~451
Vector Backbone Description: Backbone Marker:Jonathan Weissman (Addgene plasmid # 60955); Vector Backbone:pU6-sgRNA EF1Alpha-puro-T2A-BFP; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32302541
Proper citation: RRID:Addgene_164793 Copy
Species: Homo sapiens
Genetic Insert: miR-144
Vector Backbone Description: Backbone Marker:Jonathan Weissman (Addgene plasmid # 60955); Vector Backbone:pU6-sgRNA EF1Alpha-puro-T2A-BFP; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32302541
Proper citation: RRID:Addgene_164791 Copy
Species: Homo sapiens
Genetic Insert: microRNA 451a
Vector Backbone Description: Backbone Marker:Jonathan Weissman (Addgene plasmid # 60955); Vector Backbone:pU6-sgRNA EF1Alpha-puro-T2A-BFP; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32302541
Proper citation: RRID:Addgene_164792 Copy
Genetic Insert: GluN2B
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pLenti7.3DEST; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25887710
Proper citation: RRID:Addgene_164787 Copy
Species: Leptotrichia shahii
Genetic Insert: LsCas13a-gRNA-NT
Vector Backbone Description: Backbone Size:3267; Vector Backbone:pBAD18; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34694607
Comments: Two repeats without a spacer sequence for use as negative /non-targeting control.
Proper citation: RRID:Addgene_164857 Copy
Species: Homo sapiens
Genetic Insert: TLN2
Vector Backbone Description: Vector Backbone:pcDNA4.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32605925
Proper citation: RRID:Addgene_164841 Copy
Species: Homo sapiens
Genetic Insert: VCL
Vector Backbone Description: Vector Backbone:pET3; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20502710
Proper citation: RRID:Addgene_164843 Copy
Species: Aedes aegypti
Genetic Insert: 4 gRNAs and 3xP3-tdTomato
Vector Backbone Description: Backbone Size:5260; Vector Backbone:piggyBac; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34508072
Proper citation: RRID:Addgene_164848 Copy
Species: Homo sapiens
Genetic Insert: TCF4 DNA binding domain
Vector Backbone Description: Backbone Marker:Amersham; Backbone Size:5000; Vector Backbone:pGEX-2TK; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9065402
Proper citation: RRID:Addgene_16488 Copy
Species: Synthetic
Genetic Insert: Enhanced green fluorescent protein
Vector Backbone Description: Backbone Size:7140; Vector Backbone:MSCVpic2; Vector Types:Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27891214
Proper citation: RRID:Addgene_164919 Copy
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