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Species: Other
Genetic Insert: QUAS enhancer driving mNeonGreen-NES and mKate2-NLS
Vector Backbone Description: Backbone Size:7839; Vector Backbone:pDestTol2CR; Vector Types:zebrafish Tol2 transgenesis; Bacterial Resistance:Ampicillin
Defining Citation: PMID:41123438
Proper citation: RRID:Addgene_241912 Copy
Species: Other
Genetic Insert: CitA-Tq-LR
Vector Backbone Description: Vector Backbone:pRsetB; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:41071660
Proper citation: RRID:Addgene_241830 Copy
Species: Other
Genetic Insert: Alb-mStayGold
Vector Backbone Description: Backbone Marker:VVF (p438) https://vvf.ethz.ch/ ; Vector Backbone:pAAV-P3 (Addgene plasmid #183460); Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:41393514
Proper citation: RRID:Addgene_234549 Copy
Species: Other
Genetic Insert: φC31(PhiC31)
Vector Backbone Description: Backbone Marker:NA; Backbone Size:5218; Vector Backbone:pET28a(+)v2; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Kanamycin
Comments: We use a tag-free SUMO protease (ulp1) for cleaving the TwinStrep tag from the final product. Tag-free ulp1 can be synthesized using Addgene Plasmid #190063 and a His-tagged TEV protease or HRV-3C protease (PreScission protease).
The total insert size displayed on this page (2403bp) includes all tags and linkers.
Proper citation: RRID:Addgene_246982 Copy
Species: Other
Genetic Insert: KI gRNA for Nlgn2
Vector Backbone Description: Vector Backbone:pHnS; Vector Types:AAV; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_240303 Copy
Species: Other
Genetic Insert: Rluc
Vector Backbone Description: Vector Backbone:pAGM9121; Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:38769424
Proper citation: RRID:Addgene_239943 Copy
Species: Other
Genetic Insert: Csy4 recognition sequence
Vector Backbone Description: Vector Backbone:pAGM9121; Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:38769424
Proper citation: RRID:Addgene_239935 Copy
Species: Other
Genetic Insert: Csy4
Vector Backbone Description: Vector Backbone:pAGM9121; Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:38769424
Proper citation: RRID:Addgene_239934 Copy
Species: Other
Genetic Insert: dCas9
Vector Backbone Description: Vector Backbone:pAGM9121; Vector Types:CRISPR, Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:38769424
Proper citation: RRID:Addgene_239933 Copy
Species: Other
Genetic Insert: Chikungunya virus 181/25 strain structural proteins
Vector Backbone Description: Vector Backbone:pSIN; Vector Types:Sindbis virus helper construct; Bacterial Resistance:Ampicillin
Comments: Please visit https://doi.org/10.1101/2025.06.25.661405 for bioRxiv preprint.
Proper citation: RRID:Addgene_242479 Copy
Species: Other
Genetic Insert: EGFP
Vector Backbone Description: Backbone Marker:Tuttle et al Plant Methods. 2012 Aug 1;8(1):27. doi: 10.1186/1746-4811-8-27.; Vector Backbone:pJRT.Agro.CLCrVA.008; Vector Types:Plant Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:40394900
Proper citation: RRID:Addgene_234917 Copy
Species: Other
Genetic Insert: guide targeting fwYellow
Vector Backbone Description: Vector Backbone:pUC19; Vector Types:Bacterial Expression, CRISPR, Cell-free system using bacterial extract; Bacterial Resistance:Ampicillin
Defining Citation: PMID:39095367
Proper citation: RRID:Addgene_239456 Copy
Species: Other
Genetic Insert: tyrosinase
Vector Backbone Description: Vector Backbone:pUC19; Vector Types:Bacterial Expression, CRISPR, Cell-free system using bacterial extract; Bacterial Resistance:Ampicillin
Defining Citation: PMID:39095367
Proper citation: RRID:Addgene_239454 Copy
Species: Other
Genetic Insert: 35S terminator seqeunce
Vector Backbone Description: Backbone Size:2222; Vector Backbone:pICH41276; Vector Types:Plant Expression, Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:41351300
Comments: Please visit https://doi.org/10.1101/2025.06.20.660755 for bioRxiv preprint.
Proper citation: RRID:Addgene_210766 Copy
Species: Other
Genetic Insert: SpCas9
Vector Backbone Description: Backbone Size:5700; Vector Backbone:RT14; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:41131151
Comments: Please visit https://doi.org/10.1101/2024.07.21.604473 for bioRxiv preprint.
Proper citation: RRID:Addgene_237445 Copy
Species: Other
Genetic Insert: NlChR
Vector Backbone Description: Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:40970384
Comments: Please visit https://doi.org/10.1101/2025.02.24.639930 for bioRxiv preprint.
Proper citation: RRID:Addgene_232600 Copy
Species: Other
Genetic Insert: CaMV 35S::dCas9
Vector Backbone Description: Vector Backbone:Binary; Vector Types:Plant Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:38769424
Proper citation: RRID:Addgene_239940 Copy
Species: Other
Genetic Insert: CaMV 35S-SynPro-01::Rluc
Vector Backbone Description: Vector Backbone:pBluescript SK(+); Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38769424
Proper citation: RRID:Addgene_239946 Copy
Species: Other
Vector Backbone Description: Vector Backbone:n/a; Vector Types:This is a strain, not a plasmid; Bacterial Resistance:None
Defining Citation: PMID:42199375
Comments: Genotype: E. coli B F- ompT gal dcm lon hsdSB(rB–mB–) λ(DE3 [lacI lacUV5-T7p07 ind1 sam7 nin5]) [malB+]K-12(λS) ΔendA ΔrecA glvC ::[ phlFAM, cymRAM, luxR, vanRAM, lacIAM, tetR ]
Fast growth at 37C in LB (doubling time ~ 20-25 minutes) and improved DNA (endA, recA) and protein stability (lon, ompT).
Strain validation:
- PCR using OR1965 (TGGCGTAAAGGTGCTTCC) and OR1960 (ATAACCAATCAGGCTTCCTACTTACAGAATTG) generates a product of 5 518 bp.
- PCR using OR1965 (TGGCGTAAAGGTGCTTCC) and OR1160(GCTTGTCGACGACGGC) generates a product of 140 bp.
- PCR using OR1157 (TACCCCAGTTGGGGCAC) and OR1960 (ATAACCAATCAGGCTTCCTACTTACAGAATTG) generates a product of 110 bp. Please visit https://doi.org/10.1101/2025.10.29.680610 for bioRxiv preprint.
Proper citation: RRID:Addgene_235121 Copy
Species: Other
Genetic Insert: CaMV 35S::dCas9
Vector Backbone Description: Vector Backbone:pBluescript SK(+); Vector Types:CRISPR, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38769424
Proper citation: RRID:Addgene_239937 Copy
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