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Species: Homo sapiens
Genetic Insert: MRPP3
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5369; Vector Backbone:pET28-b(+); Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:18984158
Proper citation: RRID:Addgene_67864 Copy
Species: Homo sapiens
Genetic Insert: H2B-GFP
Vector Backbone Description: Vector Backbone:pHR; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26305499
Proper citation: RRID:Addgene_67928 Copy
Species: Homo sapiens
Genetic Insert: mCherry-Emi1
Vector Backbone Description: Vector Backbone:pHR; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26305499
Proper citation: RRID:Addgene_67927 Copy
Species: Homo sapiens
Genetic Insert: GFP-Aurora A
Vector Backbone Description: Vector Backbone:pHR; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26305499
Proper citation: RRID:Addgene_67924 Copy
Species: Homo sapiens
Genetic Insert: sfGFP-NLS-P2A-NLS-mCherry
Vector Backbone Description: Vector Backbone:pHR; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26305499
Proper citation: RRID:Addgene_67931 Copy
Species: Homo sapiens
Genetic Insert: sfGFP-NLS-P2A-NLS-mCherry
Vector Backbone Description: Vector Backbone:pHR; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26305499
Proper citation: RRID:Addgene_67930 Copy
Species: Homo sapiens
Genetic Insert: U6gRNA cassette, PGKpuro2AmCherry cassette, WPRE
Vector Backbone Description: Backbone Size:5783; Vector Backbone:pKLV2 lentiviral vector; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27760321
Proper citation: RRID:Addgene_67977 Copy
Species: Homo sapiens
Genetic Insert: EF1a-Cas9 cassette, WPRE
Vector Backbone Description: Backbone Size:5783; Vector Backbone:pKLV2 lentiviral vector; Vector Types:Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27760321
Proper citation: RRID:Addgene_67978 Copy
Species: Homo sapiens
Genetic Insert: U6gRNA cassette, PGKpuro2AZsG cassette, WPRE
Vector Backbone Description: Backbone Size:5783; Vector Backbone:pKLV2 lentiviral vector; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27760321
Proper citation: RRID:Addgene_67975 Copy
Species: Homo sapiens
Genetic Insert: A33
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:4800; Vector Backbone:pGL3 Basic; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12114523
Proper citation: RRID:Addgene_68149 Copy
Species: Homo sapiens
Genetic Insert: Anillin
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:18158243
Proper citation: RRID:Addgene_68027 Copy
Species: Homo sapiens
Genetic Insert: A33
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:4800; Vector Backbone:pGL3 Basic; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12114523
Proper citation: RRID:Addgene_68148 Copy
Species: Homo sapiens
Genetic Insert: U6gRNA cassette, PGKpuro2ABFP cassette, WPRE
Vector Backbone Description: Backbone Size:5783; Vector Backbone:pKLV2 lentiviral vector; Vector Types:Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27760321
Comments: This library consists of 90,709 guide sequences targeting a total of 18,010 human genes. For more information, please see the associated article.
The library is supplied as 50 ul of DNA at 20 ng/ul.
The backbone of this library is Plasmid 67974: pKLV2-U6gRNA5(BbsI)-PGKpuro2ABFP-W
Proper citation: RRID:Addgene_67989 Copy
Species: Homo sapiens
Genetic Insert: A33
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:4800; Vector Backbone:pGL3 Basic; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12114523
Proper citation: RRID:Addgene_68151 Copy
Species: Homo sapiens
Genetic Insert: U6gRNA cassette, PGKmCherryABFP cassette, WPRE
Vector Backbone Description: Backbone Size:5783; Vector Backbone:pKLV2 lentiviral vector; Vector Types:Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27760321
Proper citation: RRID:Addgene_67986 Copy
Species: Homo sapiens
Genetic Insert: U6gRNA cassette, PGKmCherry2AGFP cassette, WPRE
Vector Backbone Description: Backbone Size:5783; Vector Backbone:pKLV2 lentiviral vector; Vector Types:Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27760321
Proper citation: RRID:Addgene_67982 Copy
Species: Homo sapiens
Genetic Insert: A33
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:4800; Vector Backbone:pGL3 Basic; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12114523
Proper citation: RRID:Addgene_68157 Copy
Species: Homo sapiens
Genetic Insert: BAP1
Vector Backbone Description: Backbone Marker:Dr. J. Mirkovitch, Swiss Institute for Experimental Cancer Research, Epalinges, Switzerland; Backbone Size:5224; Vector Backbone:pCI-puro; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28389374
Comments: Described in Vega database as BAP1-010 (OTTHUMT00000351150), encodes a 711 aa long protein
Proper citation: RRID:Addgene_68366 Copy
Species: Homo sapiens
Genetic Insert: EXO1
Vector Backbone Description: Backbone Marker:NEB; Backbone Size:6700; Vector Backbone:pTXB1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21052091
Comments: Use BL21 cells for protein production.
EXO1b K589 was point-mutated to E, since this is the sequence described at UniProt (entry Q9UQ84). EXO1b also contains the following natural variants that were not corrected:
H354>R
E670>G
R723>C
Proper citation: RRID:Addgene_68269 Copy
Species: Homo sapiens
Genetic Insert: EXO1
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5500; Vector Backbone:pcDNA3.1 HisC; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15867354
Comments: EXO1b K589 was point-mutated to E, since this is the sequence described at UniProt (entry Q9UQ84). EXO1b also contains the following natural variants that were not corrected:
H354>R
E670>G
R723>C
Proper citation: RRID:Addgene_68268 Copy
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