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Species: Homo sapiens
Genetic Insert: MBP-MEK1 S218TAG
Vector Backbone Description: Vector Backbone:pCRT7/NT-TOPO; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26350500
Comments: Encodes MBP-MEK1 (human) fusion protein, with TAG at position 218 and Ser encoded at position 222 in the activation loop.
Proper citation: RRID:Addgene_68301 Copy
Species: Homo sapiens
Genetic Insert: PLK1
Vector Backbone Description: Backbone Marker:NEB; Backbone Size:6700; Vector Backbone:pTXB1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: Use BL21 cells for protein production
Proper citation: RRID:Addgene_68272 Copy
Species: Homo sapiens
Genetic Insert: PLK1
Vector Backbone Description: Backbone Marker:NEB; Backbone Size:6700; Vector Backbone:pTXB1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: Use BL21 cells for protein production
Proper citation: RRID:Addgene_68271 Copy
Species: Homo sapiens
Genetic Insert: AurA
Vector Backbone Description: Backbone Marker:NEB; Backbone Size:6700; Vector Backbone:pTXB1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16158051
Comments: Use BL21 cells for protein production.
AurA mutation V57I has been described as “common coding region polymorphism” (Egan K et al., 2004, Carcinogenesis 25:2149)
Proper citation: RRID:Addgene_68270 Copy
Species: Homo sapiens
Genetic Insert: EF1a-Cas9 cassette, WPRE
Vector Backbone Description: Backbone Size:5783; Vector Backbone:pKLV2 lentiviral vector; Vector Types:Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27760321
Proper citation: RRID:Addgene_68343 Copy
Species: Homo sapiens
Genetic Insert: Ubiquitin C Promoter
Vector Backbone Description: Backbone Size:3519; Vector Backbone:TOPO 1-4; Vector Types:Bacterial Expression, GMAP; Bacterial Resistance:Kanamycin
Defining Citation: PMID:26887506
Proper citation: RRID:Addgene_68456 Copy
Species: Homo sapiens
Genetic Insert: FRT flanked exon4 of PAX6 gene
Vector Backbone Description: Backbone Size:4800; Vector Backbone:PL552; Vector Types:Mammalian Expression, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26145478
Proper citation: RRID:Addgene_68467 Copy
Species: Homo sapiens
Genetic Insert: H2B mCherry and TAZ/WWTR1
Vector Backbone Description: Vector Backbone:pLL3.7; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_68713 Copy
Species: Homo sapiens
Genetic Insert: FKBP12-rapamycin binding (FRB) domain of MTOR
Vector Backbone Description: Backbone Marker:Amersham Pharmacia Biotech; Vector Backbone:pGEX-2T; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9933627
Proper citation: RRID:Addgene_68726 Copy
Species: Homo sapiens
Genetic Insert: Galectin-8
Vector Backbone Description: Backbone Size:5400; Vector Backbone:pET vector; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:23511477
Comments: Addgene's QC results find M56V and R184S mutations, which are naturally existing variants and not of concern.
Proper citation: RRID:Addgene_68805 Copy
Species: Homo sapiens
Genetic Insert: FOXM1b
Vector Backbone Description: Backbone Marker:Broad Insitute; Backbone Size:7667; Vector Backbone:pCW57.1; Vector Types:Mammalian Expression, Lentiviral, Doxycycline inducible; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26243836
Comments: Note: The plasmid is derived from a FOXM1b cDNA clone from Origene which contains a SNP which causes a serine to proline amino acid residue substitution. This residue change is a natural variant and does not affect plasmid function.
Proper citation: RRID:Addgene_68811 Copy
Species: Homo sapiens
Genetic Insert: FOXM1c
Vector Backbone Description: Backbone Marker:Broad Insitute; Backbone Size:7667; Vector Backbone:pCW57.1; Vector Types:Mammalian Expression, Lentiviral, Doxycycline inducible; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26243836
Proper citation: RRID:Addgene_68810 Copy
Species: Homo sapiens
Genetic Insert: MTOR
Vector Backbone Description: Backbone Size:5400; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24631838
Comments: Activating mutations in MTOR were generated using site-directed mutagenesis on AddGene plasmid 26603 pcDNA3-Flag mTOR wt deposited by Jie Chen lab
Vilella-Bach et al J Biol Chem. 1999 Feb 12. 274(7):4266-72.
Proper citation: RRID:Addgene_69013 Copy
Species: Homo sapiens
Genetic Insert: Cyclin-Dependent Kinase 19
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:8877; Vector Backbone:pLVX-EF1α-IRES-ZsGreen1; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26416749
Proper citation: RRID:Addgene_68859 Copy
Species: Homo sapiens
Genetic Insert: Cyclin-Dependent Kinase 19
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:8892; Vector Backbone:pLVX-EF1α-IRES-ZsGreen; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26416749
Proper citation: RRID:Addgene_68858 Copy
Species: Homo sapiens
Genetic Insert: cyclin-dependent kinase 8
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:8880; Vector Backbone:pLVX-EF1α-IRES-mCherry; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26416749
Proper citation: RRID:Addgene_68856 Copy
Species: Homo sapiens
Genetic Insert: PHF19
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2800; Vector Backbone:pCR8; Vector Types:Gateway Cloning; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:23160351
Proper citation: RRID:Addgene_68864 Copy
Species: Homo sapiens
Genetic Insert: CHD5
Vector Backbone Description: Vector Backbone:pCMV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23948251
Proper citation: RRID:Addgene_68870 Copy
Species: Homo sapiens
Genetic Insert: CHD5
Vector Backbone Description: Vector Backbone:pGEX; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23948251
Proper citation: RRID:Addgene_68874 Copy
Species: Homo sapiens
Genetic Insert: CHD5
Vector Backbone Description: Vector Backbone:pCMV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23948251
Proper citation: RRID:Addgene_68871 Copy
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