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Vector Backbone Description: Backbone Size:5428; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression, Gateway shuttle vector; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:29997244
Proper citation: RRID:Addgene_113448 Copy
Species: Simonsiella muelleri
Genetic Insert: Type II-C anti-CRISPR
Vector Backbone Description: Backbone Size:4097; Vector Backbone:pCSDest2; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30514786
Proper citation: RRID:Addgene_113435 Copy
Genetic Insert: FnCas12a
Vector Backbone Description: Vector Backbone:pMBP; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29449511
Proper citation: RRID:Addgene_113432 Copy
Species: Haemophilus parainfluenzae
Genetic Insert: Type II-C anti-CRISPR
Vector Backbone Description: Vector Backbone:pCSDest2t2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30514786
Proper citation: RRID:Addgene_113438 Copy
Species: Simonsiella muelleri
Genetic Insert: Type II-C anti-CRISPR
Vector Backbone Description: Backbone Size:4094; Vector Backbone:pCSDest2; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30514786
Proper citation: RRID:Addgene_113437 Copy
Species: Haemophilus parainfluenzae
Genetic Insert: type II-C anti-CRISPR
Vector Backbone Description: Backbone Size:4094; Vector Backbone:pCSDest2; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30514786
Proper citation: RRID:Addgene_113436 Copy
Species: Mus musculus
Genetic Insert: mAID-BE3
Vector Backbone Description: Backbone Size:3399; Vector Backbone:CMV; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30125268
Proper citation: RRID:Addgene_113420 Copy
Species: Homo sapiens
Genetic Insert: hA3A-eBE-Y130F
Vector Backbone Description: Backbone Size:3373; Vector Backbone:CMV; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30125268
Proper citation: RRID:Addgene_113423 Copy
Species: Ictalurus punctatus (channel catfish)
Genetic Insert: cAICDA-BE3
Vector Backbone Description: Backbone Size:3399; Vector Backbone:CMV; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30125268
Proper citation: RRID:Addgene_113422 Copy
Species: Mus musculus
Genetic Insert: mA1-BE3
Vector Backbone Description: Backbone Size:3399; Vector Backbone:CMV; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30125268
Proper citation: RRID:Addgene_113421 Copy
Species: Homo sapiens
Genetic Insert: hA3A-BE3-Y130F
Vector Backbone Description: Backbone Size:3399; Vector Backbone:CMV; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30125268
Proper citation: RRID:Addgene_113428 Copy
Species: Homo sapiens
Genetic Insert: hA3A-BE3-C101S
Vector Backbone Description: Backbone Size:3399; Vector Backbone:CMV; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30125268
Proper citation: RRID:Addgene_113426 Copy
Species: Homo sapiens
Genetic Insert: hA3A-BE3-Y132D
Vector Backbone Description: Backbone Size:3399; Vector Backbone:CMV; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30125268
Proper citation: RRID:Addgene_113425 Copy
Species: Homo sapiens
Genetic Insert: hA3A-eBE-Y132D
Vector Backbone Description: Backbone Size:3373; Vector Backbone:CMV; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30125268
Proper citation: RRID:Addgene_113429 Copy
Species: Homo sapiens
Genetic Insert: UBXD5
Vector Backbone Description: Backbone Marker:Ruedi Aebersold; Backbone Size:7017; Vector Backbone:pcDNA5-FRT-TO-cSH-GW ; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29599191
Proper citation: RRID:Addgene_113493 Copy
Species: Homo sapiens
Genetic Insert: Cezanne
Vector Backbone Description: Backbone Marker:Ruedi Aebersold; Backbone Size:7017; Vector Backbone:pcDNA5-FRT-TO-cSH-GW ; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29599191
Proper citation: RRID:Addgene_113492 Copy
Species: Homo sapiens
Genetic Insert: SVIP
Vector Backbone Description: Backbone Marker:Ruedi Aebersold; Backbone Size:7017; Vector Backbone:pcDNA5-FRT-TO-cSH-GW ; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29599191
Proper citation: RRID:Addgene_113491 Copy
Species: Homo sapiens
Genetic Insert: NMHC II-A
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEGFP-C3; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:11029059
Comments: NMHC II-A can be released by HindIII/SpeI digestion.
Please note that the original pEGFP-C3 vector was modified so that the tetracyclin regulated promoter was replaced by a CMV promoter.
Proper citation: RRID:Addgene_11347 Copy
Species: Homo sapiens
Genetic Insert: PNGase
Vector Backbone Description: Backbone Marker:Ruedi Aebersold; Backbone Size:7017; Vector Backbone:pcDNA5-FRT-TO-cSH-GW ; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29599191
Proper citation: RRID:Addgene_113490 Copy
Vector Backbone Description: Backbone Marker:see "author's map". Not the same as Clontech's pLexA.; Backbone Size:5700; Vector Backbone:pLexA; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Comments: Yeast two-hybrid DNA-binding domain vector, containing the MCS illustrated in the author's map. Use for traditional cloning for a target cDNA sequence for a yeast two-hybrid screen.
This plasmid is intended for use exclusively as a teaching resource as part of the Integrated Genomics Discovery-Based Laboratory Course. Addgene does not make any guarantee that the plasmid is suitable for research purposes.
Proper citation: RRID:Addgene_11342 Copy
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