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Species: Fusicatenibacter Saccharivorans
Genetic Insert: FsRT-Cas1(opt)-Cas2(opt)
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:3700; Vector Backbone:pET30b+; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:35549411
Proper citation: RRID:Addgene_184739 Copy
Species: Synthetic
Genetic Insert: SuperFi-Cas9
Vector Backbone Description: Backbone Marker:Addgene; Backbone Size:6470; Vector Backbone:pMJ806; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Kanamycin
Defining Citation: PMID:35236982
Proper citation: RRID:Addgene_183630 Copy
Species: Homo sapiens
Genetic Insert: MYH9
Vector Backbone Description: Backbone Marker:Agilent; Backbone Size:4401; Vector Backbone:pDEST-FLAG; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:19046415
Comments: Please note: The base at position 4200, relative to the Snapgene file, is a G instead of a T, resulting in a synonymous change p.Ala1143Ala
Proper citation: RRID:Addgene_183513 Copy
Species: Synthetic
Genetic Insert: Streptococcus pyogenes cas9
Vector Backbone Description: Backbone Size:6484; Vector Backbone:Synthetic vector; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Kanamycin
Defining Citation: PMID:35813026
Comments: The ORF of cas9 is codon optimized for E. coli expression and was synthesized by GeneArt.
The additional features (MBP, TEV sites, FLAG, 3XNLS and 12XHis tag) were either PCR amplified from various vectors or purchased as synthetic DNA fragments. All gene fragments were assembled by Gibson cloning into this customized vector.
Proper citation: RRID:Addgene_182032 Copy
Species: Synthetic
Genetic Insert: CMV:MetLuc:bGH
Vector Backbone Description: Backbone Marker:Self made; Backbone Size:2327; Vector Backbone:Alpha2; Vector Types:Mammalian Expression, Luciferase, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31836712
Comments: Insert can be released with BsmBI for further cloning into Omega level vectors.
Proper citation: RRID:Addgene_135292 Copy
Genetic Insert: NTERY02
Vector Backbone Description: Vector Backbone:pCDB180; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34385692
Proper citation: RRID:Addgene_184120 Copy
Species: Other
Genetic Insert: GPI-mEGFP
Vector Backbone Description: Vector Backbone:mEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34494547
Proper citation: RRID:Addgene_182866 Copy
Genetic Insert: NTERY08
Vector Backbone Description: Vector Backbone:pCDB180; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34385692
Proper citation: RRID:Addgene_184126 Copy
Species: E.coli
Genetic Insert: 30S ribosomal subunit protein S19
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:3669; Vector Backbone:pRSF-1b; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31761533
Proper citation: RRID:Addgene_128597 Copy
Species: E.coli
Genetic Insert: S7 E.coli ribosomal protein
Vector Backbone Description: Backbone Marker:Novagen; Vector Backbone:pRSF-1b; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31761533
Proper citation: RRID:Addgene_128592 Copy
Species: E.coli
Genetic Insert: E.coli ribosomal protein S3
Vector Backbone Description: Backbone Marker:Novagen; Vector Backbone:pRSF-1b; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31761533
Proper citation: RRID:Addgene_132949 Copy
Species: Aegilops sharonensis
Genetic Insert: pGGG_Sr-1644-1Sh
Vector Backbone Description: Backbone Marker:Hayta et al (2019) https://doi.org/10.1186/s13007-019-0503-z; Backbone Size:5722; Vector Backbone:pGolden Green Gate; Vector Types:Bacterial Expression, Plant Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:35338132
Proper citation: RRID:Addgene_164087 Copy
Species: Rattus norvegicus
Genetic Insert: iLID mRuby3 iTrkA
Vector Backbone Description: Backbone Size:3900; Vector Backbone:pEGFP N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32335036
Proper citation: RRID:Addgene_136510 Copy
Species: Mus musculus
Genetic Insert: mouse neurofilament light chain with a K363TAG mutation
Vector Backbone Description: Vector Backbone:pCMV; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:35031604
Proper citation: RRID:Addgene_182661 Copy
Species: Mus musculus
Genetic Insert: mouse neurofilament light chain with a K468TAG mutation
Vector Backbone Description: Vector Backbone:pCMV; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:35031604
Proper citation: RRID:Addgene_182663 Copy
Species: Homo sapiens
Genetic Insert: RAB25, member RAS oncogene family
Vector Backbone Description: Backbone Marker:GeneArt/Life Technologies; Backbone Size:3000; Vector Backbone:pMK-RQ; Vector Types:Mammalian Expression, Bacterial Expression, CRISPR, Donor for homologous based knock in; Bacterial Resistance:Kanamycin
Defining Citation: PMID:35708998
Comments: Here donor for DExCon Rab25 knock in. DExCon = Doxycycline-mediated endogenous gene Expression Control. Lentiviral delivery of TetOn 3G is also required for this to work.
https://figshare.com/projects/DExCon_DExogron_LUXon_on-demand_expression_control_of_endogenous_genes_reveals_differential_dynamics_of_Rab11_family_members/118281.
Please visit https://www.biorxiv.org/content/10.1101/2021.12.03.471086v1.full for bioRxiv preprint.
Proper citation: RRID:Addgene_179896 Copy
Species: Mus musculus
Genetic Insert: mouse neurofilament light chain with a K363TAG mutation
Vector Backbone Description: Vector Backbone:pCMV; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:35031604
Proper citation: RRID:Addgene_182656 Copy
Species: Mus musculus
Genetic Insert: wild type mouse neurofilament light chain
Vector Backbone Description: Vector Backbone:pCMV; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:35031604
Proper citation: RRID:Addgene_182659 Copy
Species: Mus musculus
Genetic Insert: mouse neurofilament light chain with a K468TAG mutation
Vector Backbone Description: Vector Backbone:pCMV; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:35031604
Proper citation: RRID:Addgene_182658 Copy
Species: Homo sapiens
Genetic Insert: Syx
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5236; Vector Backbone:pET-28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:35639414
Comments: Protein sequence is MGSS + HHHHHH + GGG + TEV protease cleavage (ENLYFQ*S; * = cleavage site) + human Syx (synectin-binding guanine exchange factor; aa 393-792 from accession number NP_001036128.1).
Confers kanamycin-resistance.
Backbone vector is pET-28a.
Construct design by Ryan Boyd.
Preprint: Boyd et al., bioRxiv 2021.08.26.457821, https://doi.org/10.1101/2021.08.26.457821
Proper citation: RRID:Addgene_179290 Copy
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