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Species: Multiple
Genetic Insert: Zim3-dCas9
Vector Backbone Description: Vector Backbone:pMH0006, Addgene # 135448; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36576240
Comments: Human lentiviral vector for expression of dCas9 with an N-terminal Zim3 KRAB-NLS fusion, a C-terminal HA-2xNLS, and EGFP linked by a P2A site from an EF1a promoter with an upstream ubiquitous chromatin opening element, pNM1124.
Please visit https://doi.org/10.1101/2022.07.13.499814 for bioRxiv preprint.
Proper citation: RRID:Addgene_188778 Copy
Species: Multiple
Genetic Insert: Zim3-dCas9
Vector Backbone Description: Vector Backbone:pMH0006, Addgene # 135448; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36576240
Comments: Human lentiviral vector for expression of dCas9 with an N-terminal Zim3 KRAB-NLS fusion, a C-terminal HA-2xNLS, and GFP linked by a P2A site from a SFFV promoter with an upstream ubiquitous chromatin opening element. pJB69.
Please visit https://doi.org/10.1101/2022.07.13.499814 for bioRxiv preprint.
Proper citation: RRID:Addgene_188899 Copy
Species: Multiple
Genetic Insert: dCas9-XTEN80-KRAB(Kox1)
Vector Backbone Description: Vector Backbone:pMH0006, Addgene # 135448; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36576240
Comments: Human lentiviral vector for expression of dCas9 with a C-terminal HA-2xNLS-XTEN80(linker)-KRAB(Kox1), and mCherry linked by an IRES from a SFFV promoter with an upstream ubiquitous chromatin opening element, pRAP0003.
Please visit https://doi.org/10.1101/2022.07.13.499814 for bioRxiv preprint.
Proper citation: RRID:Addgene_188770 Copy
Species: Multiple
Genetic Insert: Zim3-dCas9
Vector Backbone Description: Vector Backbone:pMH0001, Addgene # 85969; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36576240
Comments: Human lentiviral vector for expression of dCas9 with an N-terminal Zim3 KRAB-NLS fusion, a C-terminal HA-2xNLS, and EGFP linked by a P2A site from an EF1a promoter with an upstream ubiquitous chromatin opening element. pNM1123.
Please visit https://doi.org/10.1101/2022.07.13.499814 for bioRxiv preprint.
Proper citation: RRID:Addgene_188779 Copy
Species: Synthetic
Genetic Insert: EGFP
Vector Backbone Description: Vector Backbone:pMH0006, Addgene # 135448; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36576240
Comments: Human lentiviral vector for expression of EGFP from a SFFV promoter with an upstream ubiquitous chromatin opening element. pJB120.
Please visit https://doi.org/10.1101/2022.07.13.499814 for bioRxiv preprint.
Proper citation: RRID:Addgene_188900 Copy
Species: Synthetic
Genetic Insert: TRX-Avi-9S-S2 [rpoA]
Vector Backbone Description: Vector Backbone:pETM20; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35128071
Proper citation: RRID:Addgene_176223 Copy
Species: Multiple
Genetic Insert: Zim3-dCas9
Vector Backbone Description: Vector Backbone:pMH0006, Addgene # 135448; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36576240
Comments: Human lentiviral vector for expression of dCas9 with an N-terminal Zim3 KRAB-NLS fusion, a C-terminal HA-2xNLS, and mTagBFP2 linked by a P2A site from a SFFV promoter with an upstream ubiquitous chromatin opening element, pJB108.
Please visit https://doi.org/10.1101/2022.07.13.499814 for bioRxiv preprint.
Proper citation: RRID:Addgene_188767 Copy
Species: Homo sapiens
Genetic Insert: hU6-RfxCas13d-DR1-BsmBI-DR-smRNA-SapI-CS1-evopreQ1-EFS-EGFP-2A-Puro-WPRE
Vector Backbone Description: Backbone Size:-2; Vector Backbone:lentiCRISPRv2; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36550277
Comments: Please visit for https://www.biorxiv.org/content/10.1101/2022.02.02.478894v1 bioRxiv preprint.
Proper citation: RRID:Addgene_192505 Copy
Species: Discosoma sp.
Genetic Insert: red chromoprotein
Vector Backbone Description: Backbone Size:7023; Vector Backbone:pBTK402; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Tetracycline
Defining Citation: PMID:37225918
Comments: Please visit https://www.biorxiv.org/content/10.1101/2023.02.15.528778v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_191002 Copy
Vector Backbone Description: Vector Backbone:modified lentiGuide Puro backbone (Addgene plasmid # 52963) with mKate; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35670669
Proper citation: RRID:Addgene_192364 Copy
Species: Synthetic
Genetic Insert: Lenti-BCMA-OE-Blast
Vector Backbone Description: Vector Backbone:pLenti; Vector Types:Lentiviral, Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35276062
Proper citation: RRID:Addgene_192194 Copy
Species: Synthetic
Genetic Insert: Lentiviral sgRNA vector for Perturb-seq with mU6 promoter
Vector Backbone Description: Vector Backbone:pBA904; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36576240
Comments: Please visit https://doi.org/10.1101/2022.07.13.499814 for bioRxiv preprint.
Proper citation: RRID:Addgene_187241 Copy
Species: Synthetic
Genetic Insert: EGFP(N213D)
Vector Backbone Description: Vector Backbone:PiggyBac; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36198772
Comments: Please visit https://www.biorxiv.org/content/10.1101/2022.01.28.478207v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_191166 Copy
Species: Synthetic
Genetic Insert: GFP ArgSen
Vector Backbone Description: Backbone Marker:Addgene; Backbone Size:7953; Vector Backbone:pKLV2-EF1a-BsdCas9-W; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33478587
Proper citation: RRID:Addgene_184695 Copy
Species: Caenorhabditis elegans
Genetic Insert: flp-3
Vector Backbone Description: Vector Backbone:pPD95.62; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33378642
Proper citation: RRID:Addgene_191048 Copy
Species: Caenorhabditis elegans
Genetic Insert: gcy-35
Vector Backbone Description: Vector Backbone:pPD95.62; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33378642
Proper citation: RRID:Addgene_191049 Copy
Species: Synthetic
Genetic Insert: PLS-KP6
Vector Backbone Description: Vector Backbone:pET21b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36109586
Proper citation: RRID:Addgene_190966 Copy
Species: Synthetic
Genetic Insert: Synthetic construct isolate AAV-PHP.eC VP1 gene
Vector Backbone Description: Vector Backbone:pUC57-mini; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37075114
Comments: Please note that this is a non-standard rep-cap construct. It uses a tTA-TRE amplification loop to increase Capsid expression and AAV production. We find that this system increases AAV titers 1.5-5 fold (Ben Deverman, Bryan Simpson and Paul Patterson, unpublished data). This is a tet-off system, so no dox or tet is needed to turn it on. It can be used like any other rep-cap plasmid. This system should only present a problem if the rAAV genome to be packaged has a tet responsive element that directs expression of a protein that affected the health of the production cells. This work supported by NIH BRAIN Initiative grant UF1MH128336. The introduction of an XbaI restriction site for ease of cloning introduces a K449R mutation, which does not have an overt effect on vector production or transduction.
Proper citation: RRID:Addgene_201903 Copy
Species: Discosoma sp.
Genetic Insert: tdTomato
Vector Backbone Description: Backbone Size:5611; Vector Backbone:pAAV-EF1a-DIO; Vector Types:Mammalian Expression, AAV, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37828037
Comments: Please visit https://doi.org/10.1101/2022.08.29.505018 for bioRxiv preprint.
Proper citation: RRID:Addgene_202610 Copy
Species: Synthetic
Genetic Insert: monomeric nuclear Tomato
Vector Backbone Description: Backbone Size:8000; Vector Backbone:pLV; Vector Types:Mammalian Expression, Lentiviral, Synthetic Biology; Bacterial Resistance:Ampicillin
Comments: Our lab uses DH5alpha cells for growth, and recombination is generally not a problem with this lenti backbone. Use NEBstable or similar strains if recombination observed. Please visit https://www.biorxiv.org/content/10.1101/2023.11.21.568191v2 for bioRxiv preprint.
Proper citation: RRID:Addgene_172335 Copy
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