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Species: Synthetic
Genetic Insert: Engineering Reagent: ZmUbi:Csy4-P2A-TaCas9 + PvUbi1:gRNAs with Csy4 spacers
Vector Backbone Description: Vector Backbone:pTRANS_260d; Vector Types:Plant Expression, CRISPR; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28522548
Proper citation: RRID:Addgene_91150 Copy
Vector Backbone Description: Vector Backbone:pCAMBIA; Vector Types:Plant Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28522548
Comments: BsaI and Esp3I sites removed from the backbone
Proper citation: RRID:Addgene_91106 Copy
Species: Synthetic
Genetic Insert: TaCas9_D10A+H840A (dead)
Vector Backbone Description: Vector Backbone:pCAMBIA; Vector Types:Plant Expression, CRISPR; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28522548
Proper citation: RRID:Addgene_91174 Copy
Species: Synthetic
Genetic Insert: TaCas9_H840A (nickase)
Vector Backbone Description: Vector Backbone:pCAMBIA; Vector Types:Plant Expression, CRISPR; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28522548
Proper citation: RRID:Addgene_91172 Copy
Species: Synthetic
Genetic Insert: TaCas9_D10A (nickase)
Vector Backbone Description: Vector Backbone:pCAMBIA; Vector Types:Plant Expression, CRISPR; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28522548
Proper citation: RRID:Addgene_91171 Copy
Species: Synthetic
Genetic Insert: Engineering Reagent: 35S:AtCas9_dead + AtU6:gRNA
Vector Backbone Description: Vector Backbone:pTRANS_210; Vector Types:Plant Expression, CRISPR; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28522548
Proper citation: RRID:Addgene_91129 Copy
Vector Backbone Description: Vector Backbone:pCAMBIA; Vector Types:Plant Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28522548
Comments: BsaI, Esp3I, SapI and AarI sites removed from the backbone
Proper citation: RRID:Addgene_91127 Copy
Vector Backbone Description: Vector Backbone:pCAMBIA; Vector Types:Plant Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28522548
Comments: BsaI, Esp3I, SapI and AarI sites removed from the backbone
Proper citation: RRID:Addgene_91121 Copy
Vector Backbone Description: Vector Backbone:pCAMBIA; Vector Types:Plant Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28522548
Comments: BsaI, Esp3I, SapI and AarI sites removed from the backbone
Proper citation: RRID:Addgene_91122 Copy
Vector Backbone Description: Vector Backbone:pCAMBIA; Vector Types:Plant Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28522548
Comments: BsaI, Esp3I and SapI sites removed from the backbone
Proper citation: RRID:Addgene_91116 Copy
Vector Backbone Description: Vector Backbone:pCAMBIA; Vector Types:Plant Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28522548
Comments: BsaI, Esp3I and SapI sites removed from the backbone
Proper citation: RRID:Addgene_91117 Copy
Vector Backbone Description: Vector Backbone:pCAMBIA; Vector Types:Plant Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28522548
Comments: BsaI, Esp3I and SapI sites removed from the backbone
Proper citation: RRID:Addgene_91115 Copy
Vector Backbone Description: Vector Backbone:pCAMBIA; Vector Types:Plant Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28522548
Comments: BsaI, Esp3I and SapI sites removed from the backbone
Proper citation: RRID:Addgene_91112 Copy
Vector Backbone Description: Vector Backbone:pCAMBIA; Vector Types:Plant Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28522548
Proper citation: RRID:Addgene_91113 Copy
Vector Backbone Description: Vector Backbone:pCAMBIA; Vector Types:Plant Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28522548
Comments: BsaI, Esp3I and SapI sites removed from the backbone
Proper citation: RRID:Addgene_91111 Copy
Species: Other
Genetic Insert: nAtCas9_D10A+gNt_R2+gNt_F2+donor
Vector Backbone Description: Vector Backbone:pTRANS_201; Vector Types:Plant Expression, CRISPR; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28522548
Proper citation: RRID:Addgene_91189 Copy
Species: Synthetic
Genetic Insert: nAtCas9_D10A+gRNActrl+donor
Vector Backbone Description: Vector Backbone:pTRANS_201; Vector Types:Plant Expression, CRISPR; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28522548
Proper citation: RRID:Addgene_91187 Copy
Species: Other
Genetic Insert: AtCas9+gNt_R2+donor
Vector Backbone Description: Vector Backbone:pTRANS_201; Vector Types:Plant Expression, CRISPR; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28522548
Proper citation: RRID:Addgene_91188 Copy
Species: Other
Genetic Insert: nAtCas9_D10A+gNt_R2+donor
Vector Backbone Description: Vector Backbone:pTRANS_201; Vector Types:Plant Expression, CRISPR; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28522548
Proper citation: RRID:Addgene_91183 Copy
Species: Other
Genetic Insert: gRNAs targeting 58kb region in medicago truncatula
Vector Backbone Description: Vector Backbone:pDIRECT_23C; Vector Types:Plant Expression, CRISPR; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28522548
Proper citation: RRID:Addgene_91181 Copy
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