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Species: Synthetic
Genetic Insert: mitoTALEN left and right to target orf125 (orf138)
Vector Backbone Description: Vector Backbone:pK7WG2; Vector Types:TALEN; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:31285556
Comments: Tandem expression vector for right and left mitoTALENs to cut kosena-type CMS gene orf125 (also cut Ogura CMS gene orf138)
Proper citation: RRID:Addgene_158737 Copy
Species: Synthetic
Genetic Insert: TALEN ORF without TAL repeat
Vector Backbone Description: Vector Backbone:PINII; Vector Types:TALEN; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31285556
Comments: Multiste Gateway entry vector E3 compatible to be used as the final insertion vectors to make right TALEN, instead of ptCMV-136/63-VR-(NI), in the addgene Platinum Gate TALEN kit (Kit # 1000000043)
Proper citation: RRID:Addgene_158734 Copy
Species: Synthetic
Genetic Insert: TALEN ORF without TAL repeat
Vector Backbone Description: Vector Backbone:PINII; Vector Types:TALEN; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31285556
Comments: Multiste Gateway entry vector E3 compatible to be used as the final insertion vectors to make right TALEN, instead of ptCMV-136/63-VR-(NN), in the addgene Platinum Gate TALEN kit (Kit # 1000000043)
Proper citation: RRID:Addgene_158735 Copy
Species: Synthetic
Genetic Insert: TALEN ORF without TAL repeat
Vector Backbone Description: Vector Backbone:PINII; Vector Types:TALEN; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31285556
Comments: Multiste Gateway entry vector E3 compatible to be used as the final insertion vectors to make right TALEN, instead of ptCMV-136/63-VR-(HD), in the addgene Platinum Gate TALEN kit (Kit # 1000000043)
Proper citation: RRID:Addgene_158732 Copy
Species: Synthetic
Genetic Insert: TALEN ORF without TAL repeat
Vector Backbone Description: Vector Backbone:PINII; Vector Types:TALEN; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31285556
Comments: Multiste Gateway entry vector E1 compatible to be used as the final insertion vectors to make left TALEN, instead of ptCMV-136/63-VR-(NI), in the addgene Platinum Gate TALEN kit (Kit # 1000000043)
Proper citation: RRID:Addgene_158730 Copy
Species: Synthetic
Genetic Insert: TALEN ORF without TAL repeat
Vector Backbone Description: Vector Backbone:PINII; Vector Types:TALEN; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31285556
Comments: Multiste Gateway entry vector E1 compatible to be used as the final insertion vectors to make left TALEN, instead of ptCMV-136/63-VR-(NN), in the addgene Platinum Gate TALEN kit (Kit # 1000000043)
Proper citation: RRID:Addgene_158731 Copy
Species: Synthetic
Genetic Insert: GCaMP6f
Vector Backbone Description: Backbone Size:2300; Vector Backbone:bb118; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32748785
Comments: GCaMP6f was engineered in the paper above, then codon optimized for E. coli on a G-block and cloned into our bb100 backbone to facilitate constitutive expression . Please visit https://doi.org/10.1101/2020.04.23.058362 for bioRxiv preprint.
Proper citation: RRID:Addgene_158983 Copy
Species: Synthetic
Genetic Insert: Cytbow
Vector Backbone Description: Backbone Marker:Genscript inc; Backbone Size:2530; Vector Backbone:pCX; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24507188
Proper citation: RRID:Addgene_158995 Copy
Species: Synthetic
Genetic Insert: dSaCas9-VP64
Vector Backbone Description: Vector Backbone:pX601 (Addgene #61591); Vector Types:Mammalian Expression, AAV, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31108320
Proper citation: RRID:Addgene_158990 Copy
Species: Homo sapiens
Genetic Insert: GSK3 beta
Vector Backbone Description: Backbone Size:5000; Vector Backbone:pGEX; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: ORF is at 258-2237.
The Addgene Quality Control sequencing results have found silent nucleotide changes in the depositor GSK3 beta sequence as well as an amino acid change. The verified sequence matches PBD Sequence ID: 4IQ6_A and encodes an isoform of human GSK3 beta that should function as WT, please review the Addgene QC sequence before use.
Proper citation: RRID:Addgene_15898 Copy
Species: Homo sapiens
Genetic Insert: GSK3 alpha
Vector Backbone Description: Backbone Size:5000; Vector Backbone:pMT2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:2164470
Comments: ORF is at 1189-2640.
Proper citation: RRID:Addgene_15896 Copy
Vector Backbone Description: Backbone Size:3777; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Comments: See Fire Lab Vector Kit Documentation 1997. Fire Lab Miniprep Number pPD118.15, Ligation number L3781.
Proper citation: RRID:Addgene_1590 Copy
Species: Synthetic
Genetic Insert: BLRR
Vector Backbone Description: Backbone Size:7983; Vector Backbone:pLenti CMV Puro DEST (w118-1); Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32797168
Proper citation: RRID:Addgene_158958 Copy
Species: Homo sapiens
Genetic Insert: trGluc
Vector Backbone Description: Backbone Size:8644; Vector Backbone:CSCW-IRES-GFP; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32797168
Proper citation: RRID:Addgene_158959 Copy
Species: SARS-CoV-2
Genetic Insert: NSP12
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:3000; Vector Backbone:pRSFDuet-1; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32783916
Comments: Please visit https://doi.org/10.1101/2020.07.08.194084 for bioRxiv preprint.
Proper citation: RRID:Addgene_159107 Copy
Species: Synthetic
Genetic Insert: tdTomato
Vector Backbone Description: Backbone Marker:Andras Nagy; Vector Backbone:PB-CA; Vector Types:Mammalian Expression, piggyBac; Bacterial Resistance:Ampicillin
Comments: The tdTomato expression cassette insert is the same as in plasmid PB CAG-tdTomato (https://www.addgene.org/133569/).
Four nucleotides that were previously missing in the 5' end of the WPRE were added back, and SnapGene now recognizes it as WPRE.
Proper citation: RRID:Addgene_159095 Copy
Species: Geobacillus stearothermophilus
Genetic Insert: large fragment of Geobacillus stearothermophilus DNA polymerase I
Vector Backbone Description: Backbone Marker:EMD Bioscience; Backbone Size:5443; Vector Backbone:pET21a; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35433827
Comments: Please visit https://www.biorxiv.org/content/10.1101/2020.06.23.166397v2 for bioRxiv preprint.
Proper citation: RRID:Addgene_159148 Copy
Species: Homo sapiens
Genetic Insert: Cdc42 (placental isoform)
Vector Backbone Description: Backbone Size:4750; Vector Backbone:pRK5-myc; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10087266
Comments: Dominant negative version of Cdc42 (G25K isoform)
Proper citation: RRID:Addgene_15907 Copy
Species: Other
Genetic Insert: tdTomato
Vector Backbone Description: Vector Backbone:pUC57; Vector Types:Mammalian Expression, AAV, S1 knockin donor vector; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29599125
Proper citation: RRID:Addgene_159275 Copy
Species: Other
Genetic Insert: mScarlet-I
Vector Backbone Description: Backbone Marker:Naldini Lab; Backbone Size:6782; Vector Backbone:pLenti-MP2; Vector Types:Mammalian Expression, Lentiviral, Synthetic Biology; Bacterial Resistance:Ampicillin
Comments: The codons were optimized to the Human codon usage
Proper citation: RRID:Addgene_159172 Copy
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