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Species: Mus musculus
Genetic Insert: GCaMP6s-RPL10a
Vector Backbone Description: Vector Backbone:Addgene #50457; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32574560
Proper citation: RRID:Addgene_158781 Copy
Species: Mus musculus
Genetic Insert: GCaMP6f-RPL10a
Vector Backbone Description: Vector Backbone:Addgene #50457; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32574560
Proper citation: RRID:Addgene_158780 Copy
Species: Mus musculus
Genetic Insert: V2rf
Vector Backbone Description: Backbone Size:3015; Vector Backbone:pGEM-T Easy; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12620187
Comments: SpeI digestion and T7 RNA polymerase for antisense probe
Proper citation: RRID:Addgene_15880 Copy
Species: Mus musculus
Genetic Insert: mouse vasa promoter
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:5100; Vector Backbone:pSG5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17551945
Comments: The sequence the lab cloned is somewhat different from the NCBI sequence. This could be explained by different strains, etc., since they cloned the promoter from the FVB strain whereas the NCBI genome sequence is C57Bl6. Since the plasmid has been shown to be functional in transgenic mice, the depositing lab does not believe this represents a problem.
Proper citation: RRID:Addgene_15885 Copy
Species: Mus musculus
Genetic Insert: beta2-microglobulin
Vector Backbone Description: Backbone Size:3015; Vector Backbone:pGEM-T Easy; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12620187
Comments: SpeI digestion and T7 RNA polymerase for antisense probe
Proper citation: RRID:Addgene_15883 Copy
Species: Mus musculus
Genetic Insert: MKK7g2
Vector Backbone Description: Backbone Marker:invitrogen; Backbone Size:5446; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9891090
Proper citation: RRID:Addgene_14625 Copy
Species: Mus musculus
Genetic Insert: MKK7a2
Vector Backbone Description: Backbone Marker:invitrogen; Backbone Size:5446; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9891090
Proper citation: RRID:Addgene_14621 Copy
Species: Mus musculus
Genetic Insert: ZP3 promoter
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:2961; Vector Backbone:pBluescript II KS; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9609824
Proper citation: RRID:Addgene_14634 Copy
Species: Mus musculus
Genetic Insert: CD4
Vector Backbone Description: Backbone Size:3000; Vector Backbone:pBluescript; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:3260352
Proper citation: RRID:Addgene_14613 Copy
Species: Mus musculus
Genetic Insert: PECAM promoter/enhancer
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:6200; Vector Backbone:EGFP-1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:17068148
Proper citation: RRID:Addgene_14684 Copy
Species: Mus musculus
Genetic Insert: MKK7g1(K165A)
Vector Backbone Description: Backbone Marker:invitrogen; Backbone Size:5446; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9891090
Proper citation: RRID:Addgene_14675 Copy
Species: Mus musculus
Genetic Insert: ZP2
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:2961; Vector Backbone:pBluescript KS; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:1690843
Proper citation: RRID:Addgene_14645 Copy
Species: Mus musculus
Genetic Insert: ZP3
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:2961; Vector Backbone:pBluescript KS; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:3378665
Proper citation: RRID:Addgene_14646 Copy
Species: Mus musculus
Genetic Insert: beta-catenin
Vector Backbone Description: Backbone Size:6573; Vector Backbone:MSCV-IRES-GFP; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12717450
Proper citation: RRID:Addgene_14717 Copy
Species: Mus musculus
Genetic Insert: Drosha shRNA
Vector Backbone Description: Backbone Marker:OligoEngine; Backbone Size:6350; Vector Backbone:pSUPER.retro.puro; Vector Types:Mammalian Expression, Retroviral, RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17401365
Comments: shRNA sequence: GATCCCCTGC CGCAGCAGGT TAATTATTCA AGAGATAATT AACCTGCTGC GGCATTTTTG GAAC
Proper citation: RRID:Addgene_14775 Copy
Species: Mus musculus
Genetic Insert: DGCR8 shRNA
Vector Backbone Description: Backbone Marker:OligoEngine; Backbone Size:6350; Vector Backbone:pSUPER.retro.puro; Vector Types:Mammalian Expression, Retroviral, RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17401365
Comments: shRNA sequence: GATCCCCCTC TCAATGTGAC TGGATATTCA AGAGATATCC AGTCACATTG AGAGTTTTTG GAAC
Proper citation: RRID:Addgene_14773 Copy
Species: Mus musculus
Genetic Insert: DGCR8 shRNA
Vector Backbone Description: Backbone Marker:OligoEngine; Backbone Size:6350; Vector Backbone:pSUPER.retro.puro; Vector Types:Mammalian Expression, Retroviral, RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17401365
Comments: shRNA sequence: GATCCCCCCA GTGATGCAGA GGTAATTTCA AGAGAATTAC CTCTGCATCA CTGGTTTTTG GAAC
Proper citation: RRID:Addgene_14774 Copy
Species: Mus musculus
Genetic Insert: DGCR8 shRNA
Vector Backbone Description: Backbone Marker:OligoEngine; Backbone Size:6350; Vector Backbone:pSUPER.retro.puro; Vector Types:Mammalian Expression, Retroviral, RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17401365
Comments: shRNA sequence: GATCCCCCCA ATGATGACCA AGATTATTCA AGAGATAATC TTGGTCATCA TTGGTTTTTG GAAC
Proper citation: RRID:Addgene_14772 Copy
Species: Mus musculus
Genetic Insert: Smad6
Vector Backbone Description: Backbone Size:5100; Vector Backbone:pBabe puro 3; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10791980
Comments: See Author's map for more information.
Proper citation: RRID:Addgene_14835 Copy
Species: Mus musculus
Genetic Insert: Hmga2 ORFtr
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5500; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17322030
Comments: The 3' UTR of the mouse Hmga2 cDNA (BC052158) was subcloned into pCR2.1-TOPO (Invitrogen) for site-directed mutagenesis. To disrupt each let-7 complementary site, the nucleotides that paired to nucleotides 3 and 5 of the miRNA were substituted (see Author's map), using the Quikchange site-directed mutagenesis kit (Stratagene). The UTR fragment was then cloned back into the vector containing the Hmga2 cDNA (pCMV•SPORT6.1). To construct the mammalian expression vectors, Hmga2 inserts were excised from the pCMV•SPORT6.1 constructs using EcoRI restriction sites and cloned into pcDNA3.1 (Invitrogen).
Proper citation: RRID:Addgene_14794 Copy
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