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Species: Synthetic
Genetic Insert: Cas12c_4
Vector Backbone Description: Vector Backbone:pSC101 ori; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:35659325
Proper citation: RRID:Addgene_183071 Copy
Species: Lachnospiraceae bacterium
Genetic Insert: LbCas12a
Vector Backbone Description: Vector Backbone:pSC101 ori; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:35659325
Comments: The sequence of LbCas12a was from Addgene plasmid # 113431 ; http://n2t.net/addgene:113431 ; RRID:Addgene_113431
Proper citation: RRID:Addgene_183074 Copy
Species: Cowpea severe mosaic virus
Genetic Insert: CPSMV RNA1 cDNA with 4 introns
Vector Backbone Description: Backbone Marker:Qu lab; Backbone Size:7869; Vector Backbone:pAIDE (a pCambia1300 drivative); Vector Types:Plant Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:36307846
Comments: An upgraded version of this plasmid is available at Addgene. Please see Plasmid #254915 (https://www.addgene.org/254915/).
Proper citation: RRID:Addgene_184267 Copy
Species: Cowpea severe mosaic virus
Genetic Insert: CPSMV RNA2 cDNA, modified to permit foreign gene insertion between MP and L-CP
Vector Backbone Description: Backbone Marker:Qu lab; Backbone Size:7872; Vector Backbone:pAIDE (a pCambia1300 drivative); Vector Types:Plant Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:36307846
Comments: An upgraded version of this plasmid is available at Addgene. Please see Plasmid #254916 (https://www.addgene.org/254916/).
Proper citation: RRID:Addgene_184268 Copy
Species: Synthetic
Genetic Insert: mRuby2
Vector Backbone Description: Vector Backbone:MoClo-YTK; Vector Types:Yeast Expression, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34477863
Comments: Plasmid parts were synthesized or taken from the Yeast MoClo kit.
Proper citation: RRID:Addgene_183057 Copy
Species: Mus musculus
Genetic Insert: Neurog1
Vector Backbone Description: Backbone Size:3830; Vector Backbone:attB53-Pac-TRE-mCherry-attB53 (Addgene 183611); Vector Types:Mammalian Expression, RMCE; Bacterial Resistance:Kanamycin
Defining Citation: PMID:35616331
Proper citation: RRID:Addgene_183613 Copy
Species: Synthetic
Genetic Insert: LaG17 GFP nanobody SynNotch tTA
Vector Backbone Description: Backbone Size:3307; Vector Backbone:attB53-Pac-attB53 (Addgene 183610); Vector Types:Mammalian Expression, RMCE; Bacterial Resistance:Kanamycin
Defining Citation: PMID:35616331
Proper citation: RRID:Addgene_183614 Copy
Genetic Insert: tetO-mCherry
Vector Backbone Description: Backbone Size:3340; Vector Backbone:attB53-Pac-TRE-mCherry-attB53 (Addgene 183611); Vector Types:RMCE; Bacterial Resistance:Kanamycin
Defining Citation: PMID:35616331
Proper citation: RRID:Addgene_183612 Copy
Species: Synthetic
Genetic Insert: mCherry
Vector Backbone Description: Vector Backbone:MoClo-YTK; Vector Types:Yeast Expression, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34477863
Comments: Plasmid parts were synthesized or taken from the Yeast MoClo kit.
Proper citation: RRID:Addgene_183060 Copy
Species: Arabidopsis thaliana
Genetic Insert: FLS2
Vector Backbone Description: Vector Backbone:pCAMBIA2300; Vector Types:Plant Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:16510871
Proper citation: RRID:Addgene_86158 Copy
Species: Arabidopsis thaliana
Genetic Insert: FLS2
Vector Backbone Description: Vector Backbone:pCAMBIA2300; Vector Types:Plant Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:16510871
Proper citation: RRID:Addgene_86157 Copy
Vector Backbone Description: Vector Backbone:pICSL4723; Vector Types:Plant Expression; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_86176 Copy
Vector Backbone Description: Vector Backbone:pICSL4723; Vector Types:Plant Expression; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_86177 Copy
Species: Saccharopolyspora erythraea, Strain NRRL 2338
Genetic Insert: Ribonuclease inhibitor StI
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5252; Vector Backbone:pET-28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:14741361
Comments: Production plasmid for ribonuclease inhibitor and barstar
homolog StI from Saccharopolyspora erythraea. Structural gene for inhibitor StI from Saccharopolyspora
erythraea strain NRRL 2338. N-terminal serine of wild type
changed to glycine and 19 silent mutations improve production
in E. coli.
Proper citation: RRID:Addgene_8616 Copy
Species: Homo sapiens
Genetic Insert: ZNF644 homology arms
Vector Backbone Description: Vector Backbone:pFETCh_Donor; Vector Types:CRISPR; Bacterial Resistance:Kanamycin
Defining Citation: PMID:26355004
Comments: This plasmid was co-transfected along with PX458_ZNF644_1 and PX458_ZNF644_2 into HepG2 cells, and genomic editing was verified by PCR. We performed ChIP-seq on this cell line using anti-FLAG Sigma F1804 and obtained data that passed our quality tests. Immunoprecipitation with anti-FLAG Sigma F1804 followed by mass spectrometry identified ZNF644. All released data, including ChIP-seq files and validation data, are available at the ENCODE web portal: https://www.encodeproject.org/search/?searchTerm=flag&type=Experiment&lab.title=Richard+Myers%2C+HAIB . Note: This plasmid is part of the Mendenhall and Meyers CRISPR-based Tagging system to add a tag (currently using FLAG) to endogenous proteins. Please see https://www.addgene.org/crispr/tagging/ for more details.
Proper citation: RRID:Addgene_86264 Copy
Species: Homo sapiens
Genetic Insert: KLF11 homology arms
Vector Backbone Description: Vector Backbone:pFETCh_Donor; Vector Types:CRISPR; Bacterial Resistance:Kanamycin
Defining Citation: PMID:26355004
Comments: This plasmid was co-transfected along with PX458_KLF11_1 and PX458_KLF11_2 into HepG2 cells, and genomic editing was verified by PCR. The donor nucleotide sequence in the final codon of the KLF11 gene is modified to discourage sgRNA recognition, while retaining the native amino acid sequence. We performed ChIP-seq on this cell line using anti-FLAG Sigma F1804 and obtained data that passed our quality tests. Immunoprecipitation with anti-FLAG Sigma F1804 followed by mass spectrometry identified KLF11. All released data, including ChIP-seq files and validation data, are available at the ENCODE web portal: https://www.encodeproject.org/search/?searchTerm=flag&type=Experiment&lab.title=Richard+Myers%2C+HAIB . Note: This plasmid is part of the Mendenhall and Meyers CRISPR-based Tagging system to add a tag (currently using FLAG) to endogenous proteins. Please see https://www.addgene.org/crispr/tagging/ for more details.
Proper citation: RRID:Addgene_86269 Copy
Species: Homo sapiens
Genetic Insert: RFXANK homology arms
Vector Backbone Description: Vector Backbone:pFETCh_Donor; Vector Types:CRISPR; Bacterial Resistance:Kanamycin
Defining Citation: PMID:26355004
Comments: This plasmid was co-transfected along with PX458_RFXANK_1 and PX458_RFXANK_2 into HepG2 cells, and genomic editing was verified by PCR. We performed ChIP-seq on this cell line using anti-FLAG Sigma F1804 and obtained data that passed our quality tests. Immunoprecipitation with anti-FLAG Sigma F1804 followed by mass spectrometry identified RFXANK. All released data, including ChIP-seq files and validation data, are available at the ENCODE web portal: https://www.encodeproject.org/search/?searchTerm=flag&type=Experiment&lab.title=Richard+Myers%2C+HAIB . Note: This plasmid is part of the Mendenhall and Meyers CRISPR-based Tagging system to add a tag (currently using FLAG) to endogenous proteins. Please see https://www.addgene.org/crispr/tagging/ for more details.
Proper citation: RRID:Addgene_86296 Copy
Species: Homo sapiens
Genetic Insert: ZNF792 homology arms
Vector Backbone Description: Vector Backbone:pFETCh_Donor; Vector Types:CRISPR; Bacterial Resistance:Kanamycin
Defining Citation: PMID:26355004
Comments: This plasmid was co-transfected along with PX458_ZNF792_1 and PX458_ZNF792_2 into HepG2 cells, and genomic editing was verified by PCR. We performed ChIP-seq on this cell line using anti-FLAG Sigma F1804 and obtained data that passed our quality tests. Immunoprecipitation with anti-FLAG Sigma F1804 followed by mass spectrometry identified ZNF792. All released data, including ChIP-seq files and validation data, are available at the ENCODE web portal: https://www.encodeproject.org/search/?searchTerm=flag&type=Experiment&lab.title=Richard+Myers%2C+HAIB . Note: This plasmid is part of the Mendenhall and Meyers CRISPR-based Tagging system to add a tag (currently using FLAG) to endogenous proteins. Please see https://www.addgene.org/crispr/tagging/ for more details.
Proper citation: RRID:Addgene_86282 Copy
Genetic Insert: Efflux pump
Vector Backbone Description: Backbone Size:5088; Vector Backbone:pBbA5k; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25496359
Proper citation: RRID:Addgene_86202 Copy
Genetic Insert: Efflux pump
Vector Backbone Description: Vector Backbone:pBbA5k; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25496359
Comments: This plasmid carries G179V in Maqu_3494 compared to the genomic reference sequence. This mutation does not affect plasmid function.
Proper citation: RRID:Addgene_86201 Copy
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