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Species: Synthetic
Genetic Insert: C-BLa 2.0 fusion protein
Vector Backbone Description: Backbone Marker:John E. Cronan; Backbone Size:4284; Vector Backbone:pBAD322K (modified); Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28432015
Comments: TMD: Integrin alphaV mut GP, contains ApaI restriction site
Proper citation: RRID:Addgene_92060 Copy
Vector Backbone Description: Backbone Size:5552; Vector Backbone:pCDB179; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_91960 Copy
Species: Haemophilus ducreyi, strain: ATCC 27722
Genetic Insert: Putative Nicotinamide Phosphoribosyl Transferase
Vector Backbone Description: Backbone Size:5192; Vector Backbone:pET-28 a (+); Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30115969
Comments: The prs gene has L135I mutation (ctc to ata) in order to eliminate the alosteric regulation of phosphoribosyl pyrophosphate synthetase (Zakataeva et all., 2011).
Proper citation: RRID:Addgene_91950 Copy
Species: Arabidopsis thaliana
Genetic Insert: CRY2
Vector Backbone Description: Backbone Size:3950; Vector Backbone:pmCherryN1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28431041
Proper citation: RRID:Addgene_92030 Copy
Species: Arabidopsis thaliana
Genetic Insert: CRY2
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:5288; Vector Backbone:pmCherryC1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28431041
Proper citation: RRID:Addgene_92031 Copy
Genetic Insert: TALE targeting lysA with N-terminal TEV protease cleavage site
Vector Backbone Description: Vector Backbone:pBT-2; Vector Types:TALEN; Bacterial Resistance:Kanamycin
Defining Citation: PMID:26854666
Comments: TEV protease induction will result in a growth defect if the culture is not outgrown to a suitable density prior to addition of anhydrotetracycline.
Proper citation: RRID:Addgene_92021 Copy
Genetic Insert: TALE targeting sucA with N-terminal TEV protease cleavage site
Vector Backbone Description: Vector Backbone:pBT-2; Vector Types:TALEN; Bacterial Resistance:Kanamycin
Defining Citation: PMID:26854666
Comments: TEV protease induction will result in a growth defect if the culture is not outgrown to a suitable density prior to addition of anhydrotetracycline.
Proper citation: RRID:Addgene_92022 Copy
Genetic Insert: Cas9
Vector Backbone Description: Vector Backbone:pMAZ-SK; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28764701
Proper citation: RRID:Addgene_92050 Copy
Species: Homo sapiens
Genetic Insert: spastin
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:4300; Vector Backbone:pCMV-Tag; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28495799
Comments: Cloning of spastin cDNA into pCMV-Tag EcoICR I and BamH I sites removed myc tag. To detect spastin use anti-spastin antibody.The endogenous M1 Kozak sequence was replaced with an optimal Kozak sequence to prevent leaky translation of M87 spastin
Proper citation: RRID:Addgene_92365 Copy
Species: Synthetic
Genetic Insert: Ancestral AAV Capsid Anc80L65AAP
Vector Backbone Description: Backbone Size:5238; Vector Backbone:synthetic; Vector Types:Mammalian Expression, AAV, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28165475
Proper citation: RRID:Addgene_92307 Copy
Species: Homo sapiens
Genetic Insert: KIF13B
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28134340
Comments: Insert PCR-amplified from plasmid received from Dr. Athar Chishti, Tufts University School of Medicine (pEGFP-C1 with full-length GAKIN/KIF13B; Asaba et al. J. Biol. Chem. 2003, PMID: 12496241).
Addgene's sequencing results found a D813Y mutation in KIF13B compared to the reference sequence NM_015254.3. This residue is not conserved in kinesin-3 motors and is unlikely to affect plasmid function.
Proper citation: RRID:Addgene_90175 Copy
Vector Backbone Description: Backbone Marker:Philippe Moreillon (PMID 10816506); Backbone Size:6000; Vector Backbone:pAMB1 (pORI23); Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:22505686
Comments: This plasmid is a theta vector containing the P23 promoter and MCS-A. The backbone of this vector is based on pOri23- Dr. Philippe Moreillon (Centre Hospitalier Universitaire Vaudois).
Proper citation: RRID:Addgene_90195 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: SAD1
Vector Backbone Description: Backbone Marker:EMD Biosciences; Backbone Size:5368; Vector Backbone:pET28b; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:24681967
Comments: Full-length Sad1, amino acids 1–448, was cloned from S. cerevisiae S288C into the pET-28b plasmid at the Bam HI and XhoI sites, which fuses a 6-His tag to the N terminus.
Proper citation: RRID:Addgene_90297 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: SAD1
Vector Backbone Description: Backbone Marker:EMD Biosciences; Backbone Size:5368; Vector Backbone:pET28b; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:24681967
Comments: Full-length Sad1, amino acids 1–448, was cloned from S. cerevisiae S288C into the pET-28b plasmid at the Bam HI and XhoI sites, which fuses a 6-His tag to the N terminus.
Proper citation: RRID:Addgene_90299 Copy
Species: A. terreus
Genetic Insert: mttA
Vector Backbone Description: Backbone Size:5351; Vector Backbone:pMST635_BB3_L_AC_hyg_KanOri_pyrG5; Vector Types:Bacterial Expression, A niger; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28533066
Proper citation: RRID:Addgene_90292 Copy
Species: A. nidulens
Genetic Insert: trpC
Vector Backbone Description: Backbone Size:1966; Vector Backbone:BB1_L_34_syn_BsaI; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28533066
Proper citation: RRID:Addgene_90291 Copy
Species: A. niger
Genetic Insert: mbfA
Vector Backbone Description: Backbone Size:1966; Vector Backbone:BB1_L_12_syn_BsaI; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28533066
Proper citation: RRID:Addgene_90289 Copy
Vector Backbone Description: Backbone Size:5351; Vector Backbone:BB3_L_AE_hyg_KanOri_pyrG5; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28533066
Proper citation: RRID:Addgene_90282 Copy
Vector Backbone Description: Backbone Size:5351; Vector Backbone:BB3_L_AD_hyg_KanOri_pyrG5; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28533066
Proper citation: RRID:Addgene_90281 Copy
Genetic Insert: NA
Vector Backbone Description: Vector Backbone:pFlare5GdP; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:23636947
Proper citation: RRID:Addgene_90250 Copy
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