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Genetic Insert: Nme2Cas9 with two guide RNA cassettes
Vector Backbone Description: Vector Backbone:p1089: minimized AAV.sgRNA.Nme2Cas9; Vector Types:Mammalian Expression, Mouse Targeting, AAV, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34725353
Comments: Please visit https://www.biorxiv.org/content/10.1101/2020.10.09.333997v1 for BioRxiv preprint
Proper citation: RRID:Addgene_159537 Copy
Vector Backbone Description: Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:31958057
Proper citation: RRID:Addgene_159678 Copy
Species: Synthetic
Genetic Insert: Fzd7 subtype NGS Wnt
Vector Backbone Description: Vector Backbone:RKS vector; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32818433
Proper citation: RRID:Addgene_159626 Copy
Vector Backbone Description: Vector Backbone:pSpCas9(BB)-2A-GFP (PX458); Vector Types:CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34143204
Proper citation: RRID:Addgene_159655 Copy
Vector Backbone Description: Backbone Size:6229; Vector Backbone:pGREEN; Vector Types:Plant Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33274321
Comments: Empty pGREEN expression vector for cloning; HSP terminator
Proper citation: RRID:Addgene_159701 Copy
Species: Caenorhabditis elegans
Genetic Insert: pEXP[Prpl-3 | histamine | rpl-3 UTR]
Vector Backbone Description: Vector Backbone:pDEST R4-R3; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33437931
Comments: See www.wormbuilder.org for more information.
Proper citation: RRID:Addgene_159796 Copy
Species: Caenorhabditis elegans
Genetic Insert: pEXP[Pmlc-1 | ceGFP(2xNLS) | cbr-tbb-2 UTR]
Vector Backbone Description: Vector Backbone:pDEST R4-R3; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32908967
Comments: See www.wormbuilder.org for more information.
Proper citation: RRID:Addgene_159794 Copy
Vector Backbone Description: Backbone Size:9875; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Comments: See Fire Lab Vector Kit Documentation 1997. Fire Lab Miniprep Number pPD118.60, Ligation number L3808.
Proper citation: RRID:Addgene_1598 Copy
Species: Caenorhabditis elegans
Genetic Insert: pEXP[Peft-3 | mCardinal (NLS) | tbb-2 UTR]
Vector Backbone Description: Vector Backbone:pDEST R4-R3; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33298957
Comments: See www.wormbuilder.org for more information.
Proper citation: RRID:Addgene_159801 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: ADH1 promoter + polylinker + CYC1 terminator
Vector Backbone Description: Backbone Marker:Sikorski & Hieter, Genetics 122:19-27 (1989); Backbone Size:4283; Vector Backbone:pRS406; Vector Types:Bacterial Expression, Yeast Expression; Bacterial Resistance:Ampicillin
Comments: Mumberg D, Muller R, Funk M. Gene 156:119-22 (1995).
Proper citation: RRID:Addgene_15974 Copy
Species: Caenorhabditis elegans
Genetic Insert: pEXP[Psmu-2 | mosase(PATCs) | gpd-2 tagRFP-T(myr, patcs) smu-1 UTR]
Vector Backbone Description: Vector Backbone:pDEST R4-R3; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33298957
Comments: See www.wormbuilder.org for more information.
Proper citation: RRID:Addgene_159813 Copy
Vector Backbone Description: Backbone Size:4300; Vector Backbone:pFA6a-5FLAG-KanMX6; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:18729046
Comments: Template vector for a PCR based gene-tagging (5 x FLAG tag) method in S. pombe.
Proper citation: RRID:Addgene_15983 Copy
Species: Mus musculus
Genetic Insert: Th
Vector Backbone Description: Backbone Marker:Larry Zweifel (Addgene plasmid # 124844); Vector Backbone:pAAV-FLEX-SaCas9-U6-sgRNA; Vector Types:Mouse Targeting, AAV, CRISPR; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_159901 Copy
Species: Mus musculus
Genetic Insert: Akt1
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:6600; Vector Backbone:pLNCX; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11590437
Proper citation: RRID:Addgene_15990 Copy
Species: Mus musculus
Genetic Insert: Penk
Vector Backbone Description: Backbone Marker:Larry Zweifel (Addgene plasmid # 124844); Vector Backbone:pAAV-FLEX-SaCas9-U6-sgRNA; Vector Types:Mouse Targeting, AAV, CRISPR; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_159916 Copy
Species: Mus musculus
Genetic Insert: Rosa26 gRNA
Vector Backbone Description: Backbone Marker:Larry Zweifel (Addgene plasmid # 124844); Vector Backbone:pAAV-FLEX-SaCas9-U6-sgRNA; Vector Types:Mouse Targeting, AAV, CRISPR; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_159914 Copy
Species: Homo sapiens
Genetic Insert: RPL10a
Vector Backbone Description: Backbone Size:6909; Vector Backbone:pSicoR-eIF1a-mCherry; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32209477
Proper citation: RRID:Addgene_159890 Copy
Species: Homo sapiens
Genetic Insert: Akt1
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:6600; Vector Backbone:pLNCX; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11590437
Comments: The PH domain of AKT1 has been deleted.
Proper citation: RRID:Addgene_15989 Copy
Species: Oryza Sativa (Asian rice)
Genetic Insert: osTIR1
Vector Backbone Description: Backbone Marker:SYSTEM BIOSCIENCES; Vector Backbone:PiggyBac; Vector Types:Mammalian Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35235768
Comments: osTIR1 is cloned from #72834
mAID is cloned from #72827
iCasp9 is cloned from #15567
Proper citation: RRID:Addgene_160046 Copy
Species: Synthetic
Genetic Insert: mEGFP-NbALFA
Vector Backbone Description: Vector Backbone:pET28b; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33742097
Proper citation: RRID:Addgene_159986 Copy
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