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Species: Mus musculus
Genetic Insert: Hmga2 m4
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5500; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17322030
Comments: The 3' UTR of the mouse Hmga2 cDNA (BC052158) was subcloned into pCR2.1-TOPO (Invitrogen) for site-directed mutagenesis. To disrupt each let-7 complementary site, the nucleotides that paired to nucleotides 3 and 5 of the miRNA were substituted (see Author's map), using the Quikchange site-directed mutagenesis kit (Stratagene). The UTR fragment was then cloned back into the vector containing the Hmga2 cDNA (pCMV•SPORT6.1). To construct the mammalian expression vectors, Hmga2 inserts were excised from the pCMV•SPORT6.1 constructs using EcoRI restriction sites and cloned into pcDNA3.1 (Invitrogen).
Full sequence of wild-type plasmid available at Addgene plasmid #14789.
Proper citation: RRID:Addgene_14791 Copy
Species: Mus musculus
Genetic Insert: K-ras 3'UTR
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:4045; Vector Backbone:pRL-TK; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17401365
Comments: K-Ras (EMBL accession no. BC004642) mutations
correspond to a series of point mutations (“ACAGTGGAAACC” to “TGAGCGGCCAGG”)
from positions 157 to 168 of the 3’ UTR.
Proper citation: RRID:Addgene_14805 Copy
Species: Mus musculus
Genetic Insert: PERP promoter
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:4800; Vector Backbone:pGL3-Basic; Vector Types:Bacterial Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:14707288
Comments: The pPERPluc1 reporter was constructed by site-directed mutagenesis of the initial ATG of the PERP coding region (to create an NcoI site) using the Quick Change Site Directed mutagenesis kit (Stratagene), followed by joining of the 4-kb PERP promoter region to the initial ATG of luciferase in the pGL3Basic vector (Promega).
Proper citation: RRID:Addgene_14810 Copy
Species: Mus musculus
Genetic Insert: p53 R172H
Vector Backbone Description: Backbone Size:3000; Vector Backbone:pBluescript; Vector Types:Mammalian Expression, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15607980
Comments: Conditional mutant alleles of p53 were generated by introducing the LoxP-flanked transcriptional STOP cassette into intron 1 of the p53 gene. Site-directed mutagenesis was used to introduce an arg→his mutation at codon 172.
Note: This is a large plasmid that can undergo recombination. Bacterial colonies will grow slowly. If there are different colony sizes on a plate, the smaller ones are more likely to contain the correct plasmid.
Proper citation: RRID:Addgene_14854 Copy
Species: Mus musculus
Genetic Insert: FLJ23445
Vector Backbone Description: Backbone Marker:EMD Biosciences; Backbone Size:5900; Vector Backbone:pET32a; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12584197
Proper citation: RRID:Addgene_15434 Copy
Species: Mus musculus
Genetic Insert: MED30
Vector Backbone Description: Backbone Marker:EMD Biosciences; Backbone Size:5900; Vector Backbone:pET41a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:12584197
Proper citation: RRID:Addgene_15431 Copy
Species: Mus musculus
Genetic Insert: 2MT
Vector Backbone Description: Vector Backbone:pMAL-c2X; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32778831
Proper citation: RRID:Addgene_154353 Copy
Species: Mus musculus
Genetic Insert: MED31
Vector Backbone Description: Backbone Marker:EMD Biosciences; Backbone Size:5900; Vector Backbone:pET41a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:12584197
Proper citation: RRID:Addgene_15437 Copy
Species: Mus musculus
Genetic Insert: neurotensin
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:2900; Vector Backbone:pBluescript II SK(-); Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16673392
Comments: This is the targeting vector for the mouse neurotensin gene. It has an AscI site 3 bp downstream of the stop codon for cassette insertion.
Proper citation: RRID:Addgene_15461 Copy
Species: Mus musculus
Genetic Insert: Cluster of Differentiation 86
Vector Backbone Description: Backbone Size:4633; Vector Backbone:Modified pAAV-MCS; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31255486
Proper citation: RRID:Addgene_154848 Copy
Species: Mus musculus
Genetic Insert: Vitronectin
Vector Backbone Description: Backbone Size:4626; Vector Backbone:Modified pAAV-MCS; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31255486
Proper citation: RRID:Addgene_154849 Copy
Species: Mus musculus
Genetic Insert: Regulator of G protein Signaling 4
Vector Backbone Description: Backbone Size:4626; Vector Backbone:Modified pAAV-MCS; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31255486
Proper citation: RRID:Addgene_154847 Copy
Species: Mus musculus
Genetic Insert: GSDME
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:8102; Vector Backbone:pLVX-Puro; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32188940
Proper citation: RRID:Addgene_154877 Copy
Species: Mus musculus
Genetic Insert: Ccn2
Vector Backbone Description: Vector Backbone:AR52; Vector Types:Mouse Targeting; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32366677
Comments: Addgene NGS result had mutation M24T on Ccn2 [NP_034347.2], depositor confirmed that the mutation affects none of the key known functional domains and is not really a concern for the activity of the protein.
Proper citation: RRID:Addgene_155015 Copy
Species: Mus musculus
Genetic Insert: ATF4 5' UTR
Vector Backbone Description: Backbone Marker:Peter van Galen; Vector Backbone:pSMALB; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24776803
Comments: Also used in van Galen et al., 2018, Integrated Stress Response Activity Marks Stem Cells in Normal Hematopoiesis and Leukemia, Cell Reports 25, 1109–1117 (https://doi.org/10.1016/j.celrep.2018.10.021).
Proper citation: RRID:Addgene_155034 Copy
Species: Mus musculus
Genetic Insert: ATF4 5' UTR
Vector Backbone Description: Backbone Marker:Peter van Galen; Vector Backbone:pSMALB; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24776803
Comments: Also used in van Galen et al., 2018, Integrated Stress Response Activity Marks Stem Cells in Normal Hematopoiesis and Leukemia, Cell Reports 25, 1109–1117 (https://doi.org/10.1016/j.celrep.2018.10.021).
This plasmid should be used with pSMALB-ATF4.12 (negative control, https://www.addgene.org/155033/) and pSMALB-ATF4.14 (positive control), https://www.addgene.org/155034/.
Proper citation: RRID:Addgene_155032 Copy
Species: Mus musculus
Genetic Insert: ATF4 5' UTR
Vector Backbone Description: Backbone Marker:Peter van Galen; Vector Backbone:pSMALB; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24776803
Comments: Also used in van Galen et al., 2018, Integrated Stress Response Activity Marks Stem Cells in Normal Hematopoiesis and Leukemia, Cell Reports 25, 1109–1117 (https://doi.org/10.1016/j.celrep.2018.10.021).
Proper citation: RRID:Addgene_155033 Copy
Species: Mus musculus
Genetic Insert: PRDM16 shRNA
Vector Backbone Description: Backbone Marker:oligoengine; Backbone Size:7300; Vector Backbone:pSuper-Retro Neo + GFP; Vector Types:Mammalian Expression, Retroviral, RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17618855
Comments: The siRNA sequence for use in PRDM16 depletion experiments was 5'-GAAGAGCGUGAGUACAAAU-3'. The corresponding double-stranded DNA sequence was ligated into pSUPER-Retro (GFP-Neo) (OligoEngine) for retroviral shRNA expression. This siRNA sequence was able to specifically knock down PRDM16 protein and mRNA expression by over 75%.
Proper citation: RRID:Addgene_15505 Copy
Species: Mus musculus
Genetic Insert: PRDM16 (isoform 4)
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:6300; Vector Backbone:MSCV puro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17618855
Proper citation: RRID:Addgene_15504 Copy
Species: Mus musculus
Genetic Insert: (hg)RNA for deletion of mouse Ptbp exon 8 using SpCas9 and LbCas12a nucleases
Vector Backbone Description: Backbone Size:7481; Vector Backbone:pLCHKO; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32249828
Comments: U6-driven hgRNA cassette encoded on anti-sense strand
Proper citation: RRID:Addgene_155081 Copy
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