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Plasmid Name Proper Citation Insert Name Organism Bacterial Resistance Defining Citation Comments Vector Backbone Description Relevant Mutation Record Last Update Mentions Count
pFETCh_HOMEZ
 
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RRID:Addgene_86289 HOMEZ homology arms Homo sapiens Kanamycin PMID:26355004 This plasmid was co-transfected along with PX458_HOMEZ_1 and PX458_HOMEZ_2 into HepG2 cells, and genomic editing was verified by PCR. We performed ChIP-seq on this cell line using anti-FLAG Sigma F1804 and obtained data that passed our quality tests. Immunoprecipitation with anti-FLAG Sigma F1804 followed by mass spectrometry identified HOMEZ. All released data, including ChIP-seq files and validation data, are available at the ENCODE web portal: https://www.encodeproject.org/search/?searchTerm=flag&type=Experiment&lab.title=Richard+Myers%2C+HAIB . Note: This plasmid is part of the Mendenhall and Meyers CRISPR-based Tagging system to add a tag (currently using FLAG) to endogenous proteins. Please see https://www.addgene.org/crispr/tagging/ for more details. Vector Backbone:pFETCh_Donor; Vector Types:CRISPR; Bacterial Resistance:Kanamycin 2026-08-15 01:21:33 0
pUAS-MmTFP13xmyc
 
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RRID:Addgene_86371 mTfp1 Other Kanamycin PMID:26838932 Vector Backbone:pUAS; Vector Types:zebrafish; Bacterial Resistance:Kanamycin 2026-08-15 01:21:33 0
pET28a(+)/FbGH30
 
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RRID:Addgene_86463 FbGH30 Formosa B Kanamycin PMID:28213541 Backbone Marker:EMD Millipore; Backbone Size:5369; Vector Backbone:pET28a(+); Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin 2026-08-15 01:21:34 0
Flag-nls-NgAgo-GK
 
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RRID:Addgene_86435 NgAgo Natronobacterium gregoryi Kanamycin PMID:28609472 Backbone Size:4000; Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin 2026-08-15 01:21:34 0
pmCherry-PH(Btk)KD(Akt1)
 
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RRID:Addgene_86636 PH(Btk)KD(Akt1) Kanamycin PMID:28157504 Vector Backbone:pmCherry-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin 2026-08-15 01:21:36 0
pEGFP-Akt2(K14A/R25E)-Halo-PS
 
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RRID:Addgene_86632 Akt2(K14A/R25E)-Halo-PS Kanamycin PMID:28157504 Vector Backbone:pmCherry-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin 2026-08-15 01:21:36 0
pUB-Cas9
 
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RRID:Addgene_86556 Cas9 Synthetic Kanamycin PMID:28174584 See Hahn et al., 2017 Backbone Marker:Kristin Bernhardt; Backbone Size:11630; Vector Backbone:pKB65; Vector Types:Plant Expression, CRISPR; Bacterial Resistance:Kanamycin Cas9 is codon optimized for C. reinhardtii 2026-08-15 01:21:35 0
pRAF-MutL(R95F)
 
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RRID:Addgene_86608 E. coli MutL(R95F) E. coli Kanamycin PMID:27851738 Backbone Size:5400; Vector Backbone:pET29a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin R95F 2026-08-15 01:21:36 0
mCherry-UVRAG S498D
 
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RRID:Addgene_86745 human UVRAG Homo sapiens Kanamycin PMID:25533187 IMAGE: 6186860 Vector Backbone:pmCherry-1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin S498D 2026-08-15 01:21:37 0
pEGFP×2-N1
 
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1+ mentions
RRID:Addgene_86775 Kanamycin PMID:27633000 Backbone Marker:Clontech; Backbone Size:5438; Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin 2026-08-15 01:21:37 1
Flag Rab12 wild type
 
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RRID:Addgene_86787 Rab12 Homo sapiens Kanamycin PMID:25925668 Vector Backbone:pCMV; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin 2026-08-15 01:21:37 0
Flag Rab12 Q101L
 
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RRID:Addgene_86788 Rab12 Homo sapiens Kanamycin PMID:25925668 Vector Backbone:pCMV; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin Q101L, constitutively GTP-bound mutant 2026-08-15 01:21:37 0
pSkylan-NS-N1
 
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1+ mentions
RRID:Addgene_86785 Kanamycin PMID:27562163 Skylan was generated by mutating H62L of mEos3.1 Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin 2026-08-15 01:21:37 1
pBN312
 
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RRID:Addgene_86734 GFP_Frt_KO Caenorhabditis elegans Kanamycin PMID:28646043 Vector Backbone:pMLS252; Vector Types:Worm Expression; Bacterial Resistance:Kanamycin 2026-08-15 01:21:37 0
pU6-gRNA-TAZ
 
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RRID:Addgene_86688 U6-gRNA-TAZ Homo sapiens Kanamycin PMID:27929521 Backbone Marker:Life Technologies; Vector Backbone:pCR-Blunt-II; Vector Types:CRISPR; Bacterial Resistance:Kanamycin 2026-08-15 01:21:36 0
pET MBP mClover LIC cloning vector (MBP-mClover)
 
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RRID:Addgene_86930 Kanamycin This plasmid is an empty vector. Your gene can be inserted with a LIC cloning protocol. This plasmid can be used as a single-expression vector, and it is also compatible with our 2-series polycistronic destination vectors (2D, 2E, and 2Z), if co-expression with other genes is desired. To clone into this vector, add LIC tags to the 5' end of your PCR primers. Forward - 5' TACTTCCAATCCAATGCA 3' Reverse - 5' CTCCCACTACCAATGCC 3' Do NOT include a stop codon with your reverse primer. Linearize the plasmid with SspI, then gel purify. When digesting the DNA with T4 polymerase, use dGTP for the insert and dCTP for your linearized vector. More information on this vector can be found through http://qb3.berkeley.edu/qb3/macrolab/ Backbone Size:7273; Vector Backbone:pET; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin 2026-08-15 01:21:38 0
pEGFP-C1-FLAG-GFP-Ubl4A-N(1-89)
 
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RRID:Addgene_86922 UBL4A Homo sapiens Kanamycin PMID:27867533 Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin Residues from 90-157 is deleted 2026-08-15 01:21:38 0
pET MBP tdTomato LIC cloning vector (MBP-tdTomato)
 
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RRID:Addgene_86927 Kanamycin This plasmid is an empty vector. Your gene can be inserted with a LIC cloning protocol. This plasmid can be used as a single-expression vector, and it is also compatible with our 2-series polycistronic destination vectors (2D, 2E, and 2Z), if co-expression with other genes is desired. To clone into this vector, add LIC tags to the 5' end of your PCR primers. Forward - 5' TACTTCCAATCCAATGCA 3' Reverse - 5' CTCCCACTACCAATGCC 3' Do NOT include a stop codon with your reverse primer. Linearize the plasmid with SspI, then gel purify. When digesting the DNA with T4 polymerase, use dGTP for the insert and dCTP for your linearized vector. More information on this vector can be found through http://qb3.berkeley.edu/qb3/macrolab/ Backbone Size:7987; Vector Backbone:pET; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin 2026-08-15 01:21:38 0
pET30a-YedK
 
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RRID:Addgene_86884 YedK Eschericia coli Kanamycin PMID:29020633 Backbone Marker:Novagen; Backbone Size:5300; Vector Backbone:pET30a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin 2026-08-15 01:21:38 0
HOXC13(human)-pET
 
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RRID:Addgene_8679 HOXC13 Homo sapiens Kanamycin PMID:12761847 Tkatchenko et al Development 128: 1547-1558 (2001) Backbone Marker:Novagen; Backbone Size:5400; Vector Backbone:pET28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin makes protein by Coomassie blue staining 2026-08-15 01:21:37 0

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