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Species: Homo sapiens
Genetic Insert: HOMEZ homology arms
Vector Backbone Description: Vector Backbone:pFETCh_Donor; Vector Types:CRISPR; Bacterial Resistance:Kanamycin
Defining Citation: PMID:26355004
Comments: This plasmid was co-transfected along with PX458_HOMEZ_1 and PX458_HOMEZ_2 into HepG2 cells, and genomic editing was verified by PCR. We performed ChIP-seq on this cell line using anti-FLAG Sigma F1804 and obtained data that passed our quality tests. Immunoprecipitation with anti-FLAG Sigma F1804 followed by mass spectrometry identified HOMEZ. All released data, including ChIP-seq files and validation data, are available at the ENCODE web portal: https://www.encodeproject.org/search/?searchTerm=flag&type=Experiment&lab.title=Richard+Myers%2C+HAIB . Note: This plasmid is part of the Mendenhall and Meyers CRISPR-based Tagging system to add a tag (currently using FLAG) to endogenous proteins. Please see https://www.addgene.org/crispr/tagging/ for more details.
Proper citation: RRID:Addgene_86289 Copy
Species: Other
Genetic Insert: mTfp1
Vector Backbone Description: Vector Backbone:pUAS; Vector Types:zebrafish; Bacterial Resistance:Kanamycin
Defining Citation: PMID:26838932
Proper citation: RRID:Addgene_86371 Copy
Species: Formosa B
Genetic Insert: FbGH30
Vector Backbone Description: Backbone Marker:EMD Millipore; Backbone Size:5369; Vector Backbone:pET28a(+); Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28213541
Proper citation: RRID:Addgene_86463 Copy
Species: Natronobacterium gregoryi
Genetic Insert: NgAgo
Vector Backbone Description: Backbone Size:4000; Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28609472
Proper citation: RRID:Addgene_86435 Copy
Genetic Insert: PH(Btk)KD(Akt1)
Vector Backbone Description: Vector Backbone:pmCherry-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28157504
Proper citation: RRID:Addgene_86636 Copy
Genetic Insert: Akt2(K14A/R25E)-Halo-PS
Vector Backbone Description: Vector Backbone:pmCherry-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28157504
Proper citation: RRID:Addgene_86632 Copy
Species: Synthetic
Genetic Insert: Cas9
Vector Backbone Description: Backbone Marker:Kristin Bernhardt; Backbone Size:11630; Vector Backbone:pKB65; Vector Types:Plant Expression, CRISPR; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28174584
Comments: See Hahn et al., 2017
Proper citation: RRID:Addgene_86556 Copy
Species: E. coli
Genetic Insert: E. coli MutL(R95F)
Vector Backbone Description: Backbone Size:5400; Vector Backbone:pET29a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:27851738
Proper citation: RRID:Addgene_86608 Copy
Species: Homo sapiens
Genetic Insert: human UVRAG
Vector Backbone Description: Vector Backbone:pmCherry-1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25533187
Comments: IMAGE: 6186860
Proper citation: RRID:Addgene_86745 Copy
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:5438; Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:27633000
Proper citation: RRID:Addgene_86775 Copy
Species: Homo sapiens
Genetic Insert: Rab12
Vector Backbone Description: Vector Backbone:pCMV; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25925668
Proper citation: RRID:Addgene_86787 Copy
Species: Homo sapiens
Genetic Insert: Rab12
Vector Backbone Description: Vector Backbone:pCMV; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25925668
Proper citation: RRID:Addgene_86788 Copy
Vector Backbone Description: Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:27562163
Comments: Skylan was generated by mutating H62L of mEos3.1
Proper citation: RRID:Addgene_86785 Copy
Species: Caenorhabditis elegans
Genetic Insert: GFP_Frt_KO
Vector Backbone Description: Vector Backbone:pMLS252; Vector Types:Worm Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28646043
Proper citation: RRID:Addgene_86734 Copy
Species: Homo sapiens
Genetic Insert: U6-gRNA-TAZ
Vector Backbone Description: Backbone Marker:Life Technologies; Vector Backbone:pCR-Blunt-II; Vector Types:CRISPR; Bacterial Resistance:Kanamycin
Defining Citation: PMID:27929521
Proper citation: RRID:Addgene_86688 Copy
Vector Backbone Description: Backbone Size:7273; Vector Backbone:pET; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: This plasmid is an empty vector. Your gene can be inserted with a LIC cloning protocol. This plasmid can be used as a single-expression vector, and it is also compatible with our 2-series polycistronic destination vectors (2D, 2E, and 2Z), if co-expression with other genes is desired.
To clone into this vector, add LIC tags to the 5' end of your PCR primers.
Forward - 5' TACTTCCAATCCAATGCA 3'
Reverse - 5' CTCCCACTACCAATGCC 3'
Do NOT include a stop codon with your reverse primer.
Linearize the plasmid with SspI, then gel purify.
When digesting the DNA with T4 polymerase, use dGTP for the insert and dCTP for your linearized vector.
More information on this vector can be found through http://qb3.berkeley.edu/qb3/macrolab/
Proper citation: RRID:Addgene_86930 Copy
Species: Homo sapiens
Genetic Insert: UBL4A
Vector Backbone Description: Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:27867533
Proper citation: RRID:Addgene_86922 Copy
Vector Backbone Description: Backbone Size:7987; Vector Backbone:pET; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: This plasmid is an empty vector. Your gene can be inserted with a LIC cloning protocol. This plasmid can be used as a single-expression vector, and it is also compatible with our 2-series polycistronic destination vectors (2D, 2E, and 2Z), if co-expression with other genes is desired.
To clone into this vector, add LIC tags to the 5' end of your PCR primers.
Forward - 5' TACTTCCAATCCAATGCA 3'
Reverse - 5' CTCCCACTACCAATGCC 3'
Do NOT include a stop codon with your reverse primer.
Linearize the plasmid with SspI, then gel purify.
When digesting the DNA with T4 polymerase, use dGTP for the insert and dCTP for your linearized vector.
More information on this vector can be found through http://qb3.berkeley.edu/qb3/macrolab/
Proper citation: RRID:Addgene_86927 Copy
Species: Eschericia coli
Genetic Insert: YedK
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5300; Vector Backbone:pET30a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29020633
Proper citation: RRID:Addgene_86884 Copy
Species: Homo sapiens
Genetic Insert: HOXC13
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5400; Vector Backbone:pET28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:12761847
Comments: Tkatchenko et al Development 128: 1547-1558 (2001)
Proper citation: RRID:Addgene_8679 Copy
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