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Species: SARS-CoV-2
Genetic Insert: 3xFlag-His-nsp5CS-nsp9
Vector Backbone Description: Vector Backbone:pLIB (biGBac); Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34198327
Comments: Baculovirus generation using Tn7 transposition (Bac-to-Bac).
Proper citation: RRID:Addgene_169177 Copy
Species: Synthetic
Genetic Insert: nsp7
Vector Backbone Description: Vector Backbone:pBIG1b (biGBac); Vector Types:Insect Expression; Bacterial Resistance:Ampicillin and Spectinomycin
Defining Citation: PMID:34198323
Comments: Baculovirus generation using Tn7 transposition (Bac-to-Bac).
Proper citation: RRID:Addgene_169178 Copy
Species: Synthetic
Genetic Insert: nsp8
Vector Backbone Description: Vector Backbone:pBIG1c (biGBac); Vector Types:Insect Expression; Bacterial Resistance:Ampicillin and Spectinomycin
Defining Citation: PMID:34198323
Comments: Baculovirus generation using Tn7 transposition (Bac-to-Bac).
Proper citation: RRID:Addgene_169179 Copy
Species: Synthetic
Genetic Insert: nsp7-Linker-nsp8
Vector Backbone Description: Vector Backbone:pBIG1b (biGBac); Vector Types:Insect Expression; Bacterial Resistance:Ampicillin and Spectinomycin
Defining Citation: PMID:34198323
Comments: Baculovirus generation using Tn7 transposition (Bac-to-Bac).
Proper citation: RRID:Addgene_169181 Copy
Species: Mus musculus
Genetic Insert: RiboL1-jGCaMP8s
Vector Backbone Description: Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36739289
Proper citation: RRID:Addgene_169247 Copy
Species: Escherichia coli
Genetic Insert: lacI
Vector Backbone Description: Vector Backbone:pJOE; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34175462
Proper citation: RRID:Addgene_169243 Copy
Species: Escherichia coli
Genetic Insert: rhaRS
Vector Backbone Description: Vector Backbone:pJOE; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34175462
Comments: Please note that the 2bp deletion in rTBC0541 (6796bp) as compared to the depositor's sequence does not affect plasmid function.
Proper citation: RRID:Addgene_169244 Copy
Vector Backbone Description: Vector Backbone:pJOE; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34175462
Comments: Please note that the P10S mutation in the BLMT family bleomycin binding protein does not affect overall plasmid function.
Proper citation: RRID:Addgene_169245 Copy
Species: Homo sapiens
Genetic Insert: CDK5
Vector Backbone Description: Backbone Size:6500; Vector Backbone:pcmv; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_1692 Copy
Genetic Insert: mU6-sgRNA
Vector Backbone Description: Vector Backbone:pMJ114; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34016988
Proper citation: RRID:Addgene_169235 Copy
Species: Danio rerio
Genetic Insert: snail
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:3000; Vector Backbone:pBluescript SK; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: Antisense probe: cut with EcoR1 and transcribe with T3.
Sense probe: cut with BamH1 and transcribe with T7.
Plasmid History: Constructed by Greg Kelly, 1/94.
Proper citation: RRID:Addgene_16931 Copy
Species: Danio rerio
Genetic Insert: eng2
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:2900; Vector Backbone:pGEM-4; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: Antisense probe: cut with EcoR1 and transcribe with T7.
Sense probe: cut with Xba1 and transcribe with SP6.
Plasmid History:
Constructed by Greg Kelly, 11/93.
Proper citation: RRID:Addgene_16930 Copy
Species: Synthetic
Genetic Insert: 6xHis mCherry F30-Broccoli
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2665; Vector Backbone:pEXP5-NT; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35057626
Comments: Plasmid used for monitoring transcription and translation dynamics. Broccoli aptamer provides a fluorescent signal with DFHBI for mRNA level and mCherry provides fluorescent signal for protein level. Design and sequence of the Broccoli aptamer is from Filonov et al. (2015, doi: 10.1016/j.chembiol.2015.04.018).
Proper citation: RRID:Addgene_169234 Copy
Species: Synthetic
Genetic Insert: 3xFlag-His6-PLpro
Vector Backbone Description: Vector Backbone:pLIB (biGBac); Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34198325
Comments: Baculovirus generation using Tn7 transposition (Bac-to-Bac).
Proper citation: RRID:Addgene_169194 Copy
Species: Synthetic
Genetic Insert: GST-PLproCS-MBP
Vector Backbone Description: Vector Backbone:pGEX-4T-1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34198325
Proper citation: RRID:Addgene_169195 Copy
Species: Mus musculus
Genetic Insert: KRAB-Arid3a
Vector Backbone Description: Vector Backbone:SIN40C; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34570885
Proper citation: RRID:Addgene_169306 Copy
Species: Synthetic
Genetic Insert: Luciferase
Vector Backbone Description: Vector Backbone:SIN40C; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34570885
Proper citation: RRID:Addgene_169307 Copy
Species: Synthetic
Genetic Insert: Luciferase
Vector Backbone Description: Vector Backbone:SIN40C; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34570885
Proper citation: RRID:Addgene_169308 Copy
Species: Homo sapiens
Genetic Insert: RBM28 delta E8
Vector Backbone Description: Vector Backbone:p414GPD; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33941690
Proper citation: RRID:Addgene_169268 Copy
Species: Homo sapiens
Genetic Insert: RBM28 E5 WT minigene
Vector Backbone Description: Vector Backbone:pcDNA5/FRT/TO; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33941690
Proper citation: RRID:Addgene_169269 Copy
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