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Species: Homo sapiens
Genetic Insert: TARDBP mutant
Vector Backbone Description: Vector Backbone:pBI; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_107840 Copy
Species: Homo sapiens
Genetic Insert: TARDBP mutant
Vector Backbone Description: Vector Backbone:pCS2-Dest; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_107818 Copy
Species: Homo sapiens
Genetic Insert: TARDBP mutant
Vector Backbone Description: Vector Backbone:pCS2-Dest; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_107817 Copy
Species: Homo sapiens
Genetic Insert: TARDBP mutant
Vector Backbone Description: Vector Backbone:pCS2-Dest; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_107813 Copy
Species: Homo sapiens
Genetic Insert: TARDBP mutant
Vector Backbone Description: Vector Backbone:pCS2-Dest; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_107812 Copy
Species: Homo sapiens
Genetic Insert: TARDBP mutant
Vector Backbone Description: Vector Backbone:pCS2-Dest; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_107811 Copy
Species: Homo sapiens
Genetic Insert: TARDBP mutant
Vector Backbone Description: Vector Backbone:pCS2-Dest; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_107821 Copy
Species: Homo sapiens
Genetic Insert: TARDBP mutant
Vector Backbone Description: Vector Backbone:pCS2-Dest; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_107829 Copy
Species: Homo sapiens
Genetic Insert: TARDBP mutant
Vector Backbone Description: Vector Backbone:pCS2-Dest; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_107827 Copy
Species: Homo sapiens
Genetic Insert: TARDBP mutant
Vector Backbone Description: Vector Backbone:pCS2-Dest; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_107826 Copy
Species: Homo sapiens
Genetic Insert: TARDBP mutant
Vector Backbone Description: Vector Backbone:pCS2-Dest; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31653829
Proper citation: RRID:Addgene_107822 Copy
Species: Homo sapiens
Genetic Insert: TARDBP mutant
Vector Backbone Description: Vector Backbone:pCS2-Dest; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_107809 Copy
Species: Homo sapiens
Genetic Insert: TARDBP mutant
Vector Backbone Description: Vector Backbone:pBI; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: Cut with EcoRV (61bp spacer is lost) and use Gibson Cloning to insert CTD variant. Remove EcoRV during Gibson reaction to prevent unwanted aa insertion.
Proper citation: RRID:Addgene_107860 Copy
Species: Homo sapiens
Genetic Insert: U6-sgRNA B (CCR5)
Vector Backbone Description: Vector Backbone:pFYF1321; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29449507
Comments: CloE1
Proper citation: RRID:Addgene_107905 Copy
Species: Homo sapiens
Genetic Insert: U6-sgRNA A (CCR5)
Vector Backbone Description: Vector Backbone:pFYF1320; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29449507
Comments: CloE1
Proper citation: RRID:Addgene_107904 Copy
Species: Homo sapiens
Genetic Insert: PKC epsilon RNAi
Vector Backbone Description: Backbone Size:3176; Vector Backbone:pSUPER; Vector Types:Mammalian Expression, RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15024053
Comments: Constructed by ligation of the following oligonucleotide pairs to pSUPER: 5'-GATCCCCGAT CGAGCTGGCT GTCTTTTTCA AGAGAAAAGA CAGCCAGCTC GATCTTTTTG GAAA-3' and 5'-AGCTTTTCCA AAAAGATCGA GCTGGCTGTC TTTTCTCTTG AAAAAGACAG CCAGCTCGAT CGGG-3'.
Proper citation: RRID:Addgene_10798 Copy
Species: Homo sapiens
Genetic Insert: Exon 1 of wild type HTT
Vector Backbone Description: Backbone Size:3000; Vector Backbone:pAAV; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30089251
Proper citation: RRID:Addgene_107936 Copy
Species: Homo sapiens
Genetic Insert: NF-kB p49
Vector Backbone Description: Vector Backbone:pFLAG; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_107976 Copy
Species: Homo sapiens
Genetic Insert: OPNb 1038bp-17aa signal
Vector Backbone Description: Vector Backbone:pGEX-6P1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16288209
Comments: Please note that Addgene NGS returned several ambiguous bases in the OPNb ORF (1328Y, 1526R, 1532Y, 1610Y), but none of this would alter the amino acid sequence of the protein.
Proper citation: RRID:Addgene_107984 Copy
Species: Homo sapiens
Genetic Insert: mCherry:SACM1LC389S(1-520):[EAAAR]8:SACM1L(521-587)
Vector Backbone Description: Vector Backbone:pmCherry-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29461204
Comments: Note: The Sac1 insert contains a Y434F mutation compared to the closest reference sequence (NP_054735.3). The depositor has noted that this is a common point mutation that does not affect plasmid function.
Proper citation: RRID:Addgene_108140 Copy
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