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pGEX-2TK-WW-CR-W3083F/P3086A
 
Resource Report
Resource Website 		RRID:Addgene_18077 Dystrophin Homo sapiens Ampicillin PMID:10355629 Backbone Marker:Phramacia; Backbone Size:4969; Vector Backbone:pGEX-2TK (Pharmacia); Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin Second conserved Tryptophan (W) of the WW domain at amino acid position 3083 was changed to Phenylalanine (F) and Proline at position 3086 was changed to Alanine (A) 2026-08-15 01:11:28 0
T4 Lysozyme WT
 
Resource Report
Resource Website
1+ mentions 		RRID:Addgene_18110 T4 Lysozyme Bacteriophage T4 Ampicillin The BamHI site is 27 bp from the ATG and the HindIII site is 115 bp from the stop. Note that the T4 Lysozyme insert sequence provided by the depositing laboratory differs from GenBank reference sequence NP_049736.1 at two amino acids: R12G and I137R. These differences were confirmed by Addgene's sequencing results. Backbone Size:5000; Vector Backbone:pHS1403; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:11:28 2
T4 Lysozyme WT*
 
Resource Report
Resource Website
1+ mentions 		RRID:Addgene_18111 T4 Lysozyme Bacteriophage T4 Ampicillin Backbone Size:5000; Vector Backbone:pHS1403; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin No cysteines: C54T, C97A 2026-08-15 01:11:28 7
pGEX-2TK-WW-CR-d-ZZ-W3083F/P3086A
 
Resource Report
Resource Website 		RRID:Addgene_18079 Dystrophin Homo sapiens Ampicillin PMID:10355629 Backbone Marker:Phramacia; Backbone Size:4969; Vector Backbone:pGEX-2TK (Pharmacia); Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin Second conserved Tryptophan (W) of the WW domain at amino acid position 3083 was changed to Phenylalanine (F) and Proline at position 3086 was changed to Alanine (A) 2026-08-15 01:11:28 0
pRQ-Id1HA
 
Resource Report
Resource Website 		RRID:Addgene_18112 Inhibitor of differentiation 1 Mus musculus Ampicillin PMID:18378907 Backbone Marker:Generated from multiple sources; Backbone Size:6633; Vector Backbone:N/A; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin 2026-08-15 01:11:28 0
pRQ-hRasV12
 
Resource Report
Resource Website 		RRID:Addgene_18113 h-RasV12G Homo sapiens Ampicillin PMID:18378907 Backbone Marker:Constructed from multiple sources; Backbone Size:6664; Vector Backbone:N/A; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin G12V, constitutively active 2026-08-15 01:11:28 0
pCAGGS-S-B.1.1.529-FLAG
 
Resource Report
Resource Website
1+ mentions 		RRID:Addgene_180774 S-protein B.1.1.529 SARS-CoV-2 Ampicillin Vector Backbone:pCAGGS; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:11:28 1
pBABE myc-Raptor
 
Resource Report
Resource Website 		RRID:Addgene_18116 Raptor Homo sapiens Ampicillin PMID:18439900 Addgene #1859 was PCR amplified with primers containing EcoRI and XhoI, then ligated into pBABE-hygro and fully sequenced Backbone Size:5558; Vector Backbone:pBABE-hygro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin 2026-08-15 01:11:28 0
pBABE myc-Raptor S722A/S792A
 
Resource Report
Resource Website 		RRID:Addgene_18117 Raptor Homo sapiens Ampicillin PMID:18439900 Addgene #1859 was mutated at two serine residues, then PCR amplified with primers containing EcoRI and XhoI, then ligated into pBABE-hygro and fully sequenced Backbone Size:5558; Vector Backbone:pBABE-hygro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin S722A, S792A 2026-08-15 01:11:28 0
pHR-PD-1 (BTLA ITIM)-mGFP
 
Resource Report
Resource Website 		RRID:Addgene_180817 PD-1 (BTLA ITIM) Homo sapiens Ampicillin PMID:34734802 Vector Backbone:pHR; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin replaced PD-1-ITIM (VDYGEL) with BTLA-ITIM (IVYASL) 2026-08-15 01:11:28 0
pHR-BTLA (PD-1 ITIM)-mGFP
 
Resource Report
Resource Website 		RRID:Addgene_180818 BTLA (PD-1 ITIM) Homo sapiens Ampicillin PMID:34734802 Vector Backbone:pHR; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin replaced BTLA-ITIM (IVYASL) with PD-1-ITIM (VDYGEL) 2026-08-15 01:11:28 0
MSCV h c-MYC IRES GFP
 
Resource Report
Resource Website
10+ mentions 		RRID:Addgene_18119 c-MYC Homo sapiens Ampicillin The MSCV vector is described in; Hawley, R.G., Lieu, F.H.L., Fong, A.Z.C., and Hawley, T.S. Versatile retroviral vectors for potential use in gene therapy. Gene Ther. 1: 136-138, 1994. Use of the construct is described in; Park, I., Zhao, R., West, J.A., Yabuuchi, A., Huo, H., Ince, T.A., Lerou, P.H., Lensch, M.W., and Daley, G.Q. Reprogramming of human somatic cells to pluripotency with defined factors. Nature, 451, 141-146, 2008. Please note that the assembled sequence presented here is not 100% reflective of the actual sequence. See the Addgene diagnostic digest (found under the Resource Information section of the plasmid page) for additional information on the expected plasmid size. Addgene has found another HindIII site at the end of the MYC gene (see our quality control sequence). Backbone Marker:Dr. Robert Hawley of George Washington University; Backbone Size:5660; Vector Backbone:MSCV IRES GFP; Vector Types:Retroviral; Bacterial Resistance:Ampicillin The CUG which is the start site for Myc I is present, however, there is a 3bp deletion between this and the ATG start site for Myc II. 2026-08-15 01:11:28 15
pHR-PD-1 G224A-mGFP
 
Resource Report
Resource Website
1+ mentions 		RRID:Addgene_180819 PD-1 Homo sapiens Ampicillin PMID:34734802 Vector Backbone:pHR; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin G224A 2026-08-15 01:11:28 1
pGEX-6p-2-GST-PD-1icd (AA194-288, Y223F, Y248F)
 
Resource Report
Resource Website 		RRID:Addgene_180815 PD-1int (AA194-288, Y223F, Y248F) Homo sapiens Ampicillin PMID:32437509 Vector Backbone:pGEX-6p-2; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin PD-1 (Y223F, Y248F) 2026-08-15 01:11:28 0
pcDNA3.1-α-COPI-WD40-Arg57Ala
 
Resource Report
Resource Website 		RRID:Addgene_180759 α-COPI-WD40-Arg57Ala Schizosaccharomyces pombe Ampicillin PMID:35136165 Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin Encodes residues 1-327; mutations: Arg57Ala, Leu181Lys, Leu185Lys, Ile192Lys, Leu196Lys and Phe197Lys 2026-08-15 01:11:27 0
pBEL2308
 
Resource Report
Resource Website 		RRID:Addgene_181818 LetB (a.k.a. b1834, yebT, ECK1833) E. coli Ampicillin PMID:35077766 Backbone Marker:Novagen; Backbone Size:5523; Vector Backbone:pET21a; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin LetB(43-877; delta392-515)-6xHis 2026-08-15 01:11:29 0
pBEL2309
 
Resource Report
Resource Website 		RRID:Addgene_181819 LetB (a.k.a. b1834, yebT, ECK1833) E. coli Ampicillin PMID:35077766 Backbone Marker:Novagen; Backbone Size:5523; Vector Backbone:pET21a; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin LetB(43-877; delta516-633)-6xHis 2026-08-15 01:11:29 0
pY2ShHELIX_sgRNA_entry (CJT30)
 
Resource Report
Resource Website 		RRID:Addgene_181781 Y2-nAniI-ShTnsB, ShTnsC, ShTniQ, ShCas12k Synthetic Ampicillin PMID:36593413 Please visit https://www.biorxiv.org/content/10.1101/2022.01.07.475005v1 for bioRxiv preprint. Vector Backbone:pUC19; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin Y2-nAniI = F13Y, S111Y, K227M, F80K, L232K 2026-08-15 01:11:29 0
pBEL1749
 
Resource Report
Resource Website 		RRID:Addgene_181820 LetB (a.k.a. b1834, yebT, ECK1833) E. coli Ampicillin PMID:35077766 Backbone Marker:Novagen; Backbone Size:5523; Vector Backbone:pET21a; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin LetB(43-877; delta747-877)-6xHis 2026-08-15 01:11:29 0
pShCAST(Cas12k-TnsC)-sgRNA_entry (CJT28)
 
Resource Report
Resource Website 		RRID:Addgene_181789 ShTnsB, ShTniQ, ShCas12k-ShTnsC Synthetic Ampicillin PMID:36593413 Please visit https://www.biorxiv.org/content/10.1101/2022.01.07.475005v1 for bioRxiv preprint. Vector Backbone:pUC19; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin nAniI = K227M, F80K, L232K 2026-08-15 01:11:29 0

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