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Species: Mus musculus
Genetic Insert: nCas9(H840A)-M-MLV
Vector Backbone Description: Backbone Marker:Qi-Jun Chen; Vector Backbone:pHUE411 (modified from pCambia); Vector Types:Plant Expression, CRISPR; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33767395
Proper citation: RRID:Addgene_170131 Copy
Species: Synthetic
Genetic Insert: deGFP
Vector Backbone Description: Backbone Size:2400; Vector Backbone:pBEST; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34985758
Proper citation: RRID:Addgene_170096 Copy
Species: E. coli
Genetic Insert: E. coli type I-E Cse2
Vector Backbone Description: Backbone Size:5230; Vector Backbone:pET28b; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34985758
Comments: If used in a publication, please acknowledge that the plasmid pEcCse2 was generated by U.S. Department of Energy Joint Genome Institute, a DOE Office of Science User Facility. The associated work was supported by the Office of Science of the U.S. Department of Energy under Contract No. DE-AC02-05CH11231.
Proper citation: RRID:Addgene_170094 Copy
Species: Homo sapiens
Genetic Insert: MS2-ADAR2(3'UAG)-MS2
Vector Backbone Description: Backbone Marker:Addgene #52961; Backbone Size:10500; Vector Backbone:lentiCRISPR v2; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35044296
Proper citation: RRID:Addgene_170128 Copy
Species: Homo sapiens
Genetic Insert: MS2-ADAR2(5'UAG)-MS2
Vector Backbone Description: Backbone Marker:Addgene #52961; Backbone Size:10500; Vector Backbone:lentiCRISPR v2; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35044296
Proper citation: RRID:Addgene_170126 Copy
Species: Bacteria
Genetic Insert: Green fluorescent protein
Vector Backbone Description: Backbone Size:2076; Vector Backbone:pZa; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:31536326
Proper citation: RRID:Addgene_170081 Copy
Genetic Insert: E. coli type I-E single spacer array
Vector Backbone Description: Backbone Size:1688; Vector Backbone:ColE1; Vector Types:CRISPR, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:34985758
Proper citation: RRID:Addgene_170088 Copy
Species: Homo sapiens
Genetic Insert: Circular 200,100 guide RNA with 8bp bulge loops
Vector Backbone Description: Backbone Marker:Custom; Backbone Size:6000; Vector Backbone:AAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Kanamycin
Defining Citation: PMID:35145312
Proper citation: RRID:Addgene_170121 Copy
Species: Mus musculus
Genetic Insert: Circular 200,100 guide RNA
Vector Backbone Description: Backbone Marker:Addgene #78601; Backbone Size:5700; Vector Backbone:pZac2.1; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35145312
Proper citation: RRID:Addgene_170122 Copy
Species: Bacteria
Genetic Insert: Green fluorescent protein
Vector Backbone Description: Backbone Size:2078; Vector Backbone:pZa; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:31536326
Proper citation: RRID:Addgene_170084 Copy
Species: Branchiostoma lanceolatum
Genetic Insert: mNeonGreen
Vector Backbone Description: Vector Backbone:pSC101*; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Comments: Peptide display fusion protein sequence was obtained from Bryan Davies and was originally published in Tucker et al. 2018 (DOI:10.1016/j.cell.2017.12.009).
Tucker AT, Leonard SP, DuBois CD, Knauf GA, Cunningham AL, Wilke CO, Trent MS, Davies BW. Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries. Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. PMID: 29307492; PMCID: PMC5786472. Please visit https://www.biorxiv.org/content/10.1101/2021.06.01.446581v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_170039 Copy
Species: Homo sapiens
Genetic Insert: FKBP DD (L106P)-UbK0G75S
Vector Backbone Description: Backbone Size:0; Vector Backbone:pBMN; Vector Types:Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33007216
Proper citation: RRID:Addgene_170157 Copy
Genetic Insert: RESCUE-DDC-λN-NES
Vector Backbone Description: Backbone Marker:Custom; Backbone Size:6000; Vector Backbone:AAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Kanamycin
Defining Citation: PMID:35044296
Proper citation: RRID:Addgene_170154 Copy
Species: Homo sapiens
Genetic Insert: BoxB-RAB7A(ACG)-MS2
Vector Backbone Description: Backbone Marker:Custom; Backbone Size:6000; Vector Backbone:AAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Kanamycin
Defining Citation: PMID:35044296
Proper citation: RRID:Addgene_170155 Copy
Species: Homo sapiens
Genetic Insert: NES-MCP-RESCUE-DDN
Vector Backbone Description: Backbone Marker:Custom; Backbone Size:6000; Vector Backbone:AAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Kanamycin
Defining Citation: PMID:35044296
Proper citation: RRID:Addgene_170153 Copy
Species: Homo sapiens
Genetic Insert: chromodomain helicase DNA binding protein 7
Vector Backbone Description: Backbone Size:4776; Vector Backbone:pFastBac1; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23134727
Proper citation: RRID:Addgene_170145 Copy
Species: Homo sapiens
Genetic Insert: chromodomain helicase DNA binding protein 7
Vector Backbone Description: Backbone Size:4776; Vector Backbone:pFastBac1; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23134727
Proper citation: RRID:Addgene_170143 Copy
Genetic Insert: ChroME2s-ST-P2A-NLS-mRuby3
Vector Backbone Description: Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35120626
Proper citation: RRID:Addgene_170177 Copy
Genetic Insert: ChroME2s-P2A-H2B-mRuby3
Vector Backbone Description: Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35120626
Proper citation: RRID:Addgene_170163 Copy
Species: Homo sapiens
Genetic Insert: MAPK9
Vector Backbone Description: Backbone Size:9514; Vector Backbone:Unknown; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34646987
Proper citation: RRID:Addgene_170238 Copy
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