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Vector Backbone Description: Backbone Marker:Connie Cepko Lab (Addgene #11159); Vector Backbone:pCAGIG; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:28114302
Proper citation: RRID:Addgene_92015 Copy
Vector Backbone Description: Backbone Size:10044; Vector Backbone:pFUSE-DEST_R1R4; Vector Types:Mammalian Expression, Lentiviral, Gateway Cloning; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:28512244
Comments: Recombine Gene A + Gene B into this Destination vector by Gateway LR reaction to make the final fusion.
The pFUSE-A_P1P2 is the same plasmid as Gateway™ pDONR™221 Vector and is commercially available from ThermoFisher Scientific (Cat#: 12536017).
Proper citation: RRID:Addgene_97186 Copy
Vector Backbone Description: Backbone Size:9185; Vector Backbone:pNOC-Flux; Vector Types:Algae, Nannochloropsis; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:30883973
Proper citation: RRID:Addgene_98142 Copy
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:4283; Vector Backbone:pGL4.23; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:24413736
Comments: Gateway cassette was inserted between SacI and XhoI restriction sites in the MCS.
Proper citation: RRID:Addgene_60323 Copy
Vector Backbone Description: Vector Backbone:pPB-PGK; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:25215486
Proper citation: RRID:Addgene_60436 Copy
Vector Backbone Description: Backbone Size:7105; Vector Backbone:pDEST-0GWA; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:23471584
Proper citation: RRID:Addgene_60556 Copy
Vector Backbone Description: Backbone Size:5428; Vector Backbone:pcDNA3.1(+); Vector Types:Mammalian Expression, Gateway Cloning; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:26264872
Proper citation: RRID:Addgene_78855 Copy
Vector Backbone Description: Backbone Size:5428; Vector Backbone:pcDNA3.1(+); Vector Types:Mammalian Expression, Gateway Cloning; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:26264872
Proper citation: RRID:Addgene_78857 Copy
Vector Backbone Description: Backbone Marker:Basi, et al., PMID 8422997; Vector Backbone:pREP-X; Vector Types:Yeast Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Comments: MCS is in front of attR1 element and contains SpeI and NdeI restriction sites. nmt1/nmt1*/nmt1** - thiamine repressible promoter wt/weak/very weak, correspondingly. MCS-attR1-CmR-ccdB-attR2 cassette was introduced into pREP-X vectors by XhoI sites.
Proper citation: RRID:Addgene_79021 Copy
Vector Backbone Description: Backbone Marker:Basi, et al., PMID 8422997; Vector Backbone:pREP-X; Vector Types:Yeast Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Comments: NdeI site between V5 tag and attR1 element can be used to introduce additional tag(s) by conventional cloning. nmt1/nmt1*/nmt1** - thiamine repressible promoter wt/weak/very weak, correspondingly. 2xV5-attR1-CmR-ccdB-attR2 cassette was introduced into pREP-X vectors by XhoI sites
Proper citation: RRID:Addgene_79020 Copy
Vector Backbone Description: Backbone Marker:Basi, et al., PMID 8422997; Vector Backbone:pREP-X; Vector Types:Yeast Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Comments: MCS is in front of attR1 element and contains SpeI restriction site.
Please note- the NdeI site has been disrupted in this plasmid. The NdeI site (CATATA instead of CATATG) will generate His-Ile instead of His-Met upon translation to protein. This region is linker between N-tag and protein of interest, so this should not affect protein function.
nmt1/nmt1*/nmt1** - thiamine repressible promoter wt/weak/very weak, correspondingly. MCS-attR1-CmR-ccdB-attR2 cassette was introduced into pREP-X vectors by XhoI sites.
Proper citation: RRID:Addgene_79023 Copy
Vector Backbone Description: Backbone Marker:Basi, et al., PMID 8422997; Vector Backbone:pREP-X; Vector Types:Yeast Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Comments: MCS is in front of attR1 element and contains SpeI and NdeI restriction sites. nmt1/nmt1*/nmt1** - thiamine repressible promoter wt/weak/very weak, correspondingly. MCS-attR1-CmR-ccdB-attR2 cassette was introduced into pREP-X vectors by XhoI sites.
Proper citation: RRID:Addgene_79025 Copy
Vector Backbone Description: Backbone Marker:Basi, et al., PMID 8422997; Vector Backbone:pREP-X; Vector Types:Yeast Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Comments: NdeI site between V5 tag and attR1 element can be used to introduce additional tag(s) by conventional cloning. nmt1/nmt1*/nmt1** - thiamine repressible promoter wt/weak/very weak, correspondingly. 2xV5-attR1-CmR-ccdB-attR2 cassette was introduced into pREP-X vectors by XhoI sites
Proper citation: RRID:Addgene_79017 Copy
Vector Backbone Description: Backbone Marker:Basi, et al., PMID 8422997; Vector Backbone:pREP-X; Vector Types:Yeast Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Comments: NdeI site between V5 tag and attR1 element can be used to introduce additional tag(s) by conventional cloning. nmt1/nmt1*/nmt1** - thiamine repressible promoter wt/weak/very weak, correspondingly. 2xV5-attR1-CmR-ccdB-attR2 cassette was introduced into pREP-X vectors by XhoI sites
Proper citation: RRID:Addgene_79016 Copy
Genetic Insert: FLAG
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:7500; Vector Backbone:pDEST26; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:17846994
Proper citation: RRID:Addgene_79275 Copy
Vector Backbone Description: Backbone Marker:cloned in house; Backbone Size:7800; Vector Backbone:pSBDEST.B; Vector Types:Promoterless Gateway Destination vector; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:27694838
Comments: Sleeping Beauty compatible, promoterless Gateway Destination Vector, for MultiSite Gateway cloning.
Proper citation: RRID:Addgene_79460 Copy
Genetic Insert: pAtU6-ccdB-sgRNA
Vector Backbone Description: Vector Backbone:pUC57; Vector Types:CRISPR, Synthetic Biology; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:27579989
Proper citation: RRID:Addgene_79469 Copy
Vector Backbone Description: Backbone Marker:cloned in house; Backbone Size:8212; Vector Backbone:pSBDEST.N; Vector Types:Promoterless Gateway Destination vector; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:27694838
Comments: Sleeping Beauty compatible, promoterless Gateway Destination Vector, for MultiSite Gateway cloning.
Proper citation: RRID:Addgene_79468 Copy
Genetic Insert: pAtU6-ccdB-sgRNA
Vector Backbone Description: Vector Backbone:pUC57; Vector Types:CRISPR, Synthetic Biology; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:27579989
Proper citation: RRID:Addgene_79465 Copy
Genetic Insert: pAtU6-ccdB-sgRNA
Vector Backbone Description: Vector Backbone:pUC57; Vector Types:CRISPR, Synthetic Biology; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:27579989
Proper citation: RRID:Addgene_79467 Copy
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