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Species: Xanthomonas oryzae
Genetic Insert: repeat NH6
Vector Backbone Description: Backbone Size:3013; Vector Backbone:pTC14; Vector Types:; Bacterial Resistance:Tetracycline
Defining Citation: PMID:21493687
Proper citation: RRID:Addgene_40857 Copy
Species: Xanthomonas oryzae
Genetic Insert: repeat NH4
Vector Backbone Description: Backbone Size:3013; Vector Backbone:pTC14; Vector Types:; Bacterial Resistance:Tetracycline
Defining Citation: PMID:21493687
Proper citation: RRID:Addgene_40855 Copy
Species: Xanthomonas oryzae
Genetic Insert: repeat NH2
Vector Backbone Description: Backbone Size:3013; Vector Backbone:pTC14; Vector Types:; Bacterial Resistance:Tetracycline
Defining Citation: PMID:21493687
Proper citation: RRID:Addgene_40853 Copy
Species: Xanthomonas oryzae
Genetic Insert: repeat NH8
Vector Backbone Description: Backbone Size:3013; Vector Backbone:pTC14; Vector Types:; Bacterial Resistance:Tetracycline
Defining Citation: PMID:21493687
Proper citation: RRID:Addgene_40859 Copy
Species: S. enterica serovar Typhimurium LT2
Genetic Insert: thiosulfate reductase
Vector Backbone Description: Backbone Marker:JM Blatny; Backbone Size:0; Vector Backbone:pJB866; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Tetracycline
Defining Citation: PMID:10966412
Proper citation: RRID:Addgene_19590 Copy
Species: synthetic
Genetic Insert: lox/Frt/-PGK/EM7pm-G418/Kan-lox/Frt cassette flanked by Tn7L/R
Vector Backbone Description: Backbone Marker:New England Biolabs; Backbone Size:3000; Vector Backbone:pGPS21; Vector Types:in vitro transposion; Bacterial Resistance:Tetracycline
Defining Citation: PMID:15784610
Comments: The plasmid confers Kan resistance (25ug/ml) and Tet resistance (12.5ug/ml). However, it requires pir factor for replication.
Note that this plasmid is supplied on a stab with only Tet. When re-plating, end users should add Kan as well. Also note that the plasmid is only considered high copy in the pir-116 strain. In a WT pir strain, it is considered low copy.
Proper citation: RRID:Addgene_27180 Copy
Species: Homo sapiens
Genetic Insert: DUSP6
Vector Backbone Description: Backbone Marker:Thermo Scientific; Backbone Size:11000; Vector Backbone:pLEX-HA-MYC; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Tetracycline
Defining Citation: PMID:23839489
Comments: Catalytically inactive DUSP6
Proper citation: RRID:Addgene_27977 Copy
Species: Homo sapiens
Genetic Insert: DUSP6
Vector Backbone Description: Backbone Marker:Thermo Scientific; Backbone Size:11000; Vector Backbone:pLEX-MCS; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Tetracycline
Defining Citation: PMID:23839489
Proper citation: RRID:Addgene_27975 Copy
Species: Homo sapiens
Genetic Insert: SC4MOL
Vector Backbone Description: Backbone Size:11000; Vector Backbone:pLEX; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Tetracycline
Comments: H173Q caused by 519T->A mutation as described in He et al., J Clin Invest. 2011;121(3):976–984. PMID: 21285510
Proper citation: RRID:Addgene_27984 Copy
Species: Homo sapiens
Genetic Insert: SC4MOL
Vector Backbone Description: Backbone Size:11000; Vector Backbone:pLEX; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Tetracycline
Proper citation: RRID:Addgene_27983 Copy
Vector Backbone Description: Backbone Size:8493; Vector Backbone:n/a; Vector Types:Bacterial Expression; Bacterial Resistance:Tetracycline
Defining Citation: PMID:11483365
Comments: Note that Addgene's sequencing results found a 1 nucleotide deletion at bp# 4286 when compared with the sequence provided by the depositing laboratory. This deletion is not known to affect plasmid function.
Proper citation: RRID:Addgene_28035 Copy
Vector Backbone Description: Backbone Size:5454; Vector Backbone:pBMTBX-4; Vector Types:Bacterial Expression; Bacterial Resistance:Tetracycline
Defining Citation: PMID:20148414
Proper citation: RRID:Addgene_26075 Copy
Species: cloning vector
Genetic Insert: pStart-T2
Vector Backbone Description: Backbone Size:4245; Vector Backbone:pACYC184; Vector Types:Mouse Targeting; Bacterial Resistance:Tetracycline
Defining Citation: PMID:18546598
Proper citation: RRID:Addgene_20347 Copy
Vector Backbone Description: Backbone Marker:Addgene plasmid 43829; Backbone Size:5173; Vector Backbone:pRE107 (modified from pGP704); Vector Types:Bacterial Expression, Bacterial allelic exchange vector with sacB1; Bacterial Resistance:Tetracycline
Defining Citation: PMID:9511756
Comments: The plasmids deposited here comprise a set of SacB1-dependent allelic exchange vectors improved from a previously described suicide vector, pGP704 (Miller and Mekalanos, 1988), by including a system to select for plasmid loss by recombination.
Plasmid pRE107 (Addgene plasmid #43829) was constructed by cloning the appropriate EcoRI fragment from pUC58-sacB1 (McIver et al., 1995) into pGP704 (see associated schematic image). The sacB1 allele is a modified variation of sacB, where unique restriction sites were removed by site directed mutagenesis (McIver et al., 1995). Additionally, the BamHI site in the R6K ori of the resulting plasmid was removed by partial BamHI digestion, blunting the resulting overhangs with PolIk (resulting in the formation of a ClaI site) and screening for its loss by restriction analysis.
To use this plasmid with bla fusions and increase the functionality of this system, the depositing laboratory replaced the ApR gene as follows.
The ApR gene flanked by the remaining BamHI sites was removed and replaced with either CmR, TcR or KmR resistance markers (see associated schematic image).
The TcR gene was cloned on a blunted EcoRI–StyI fragment from pBR322 into the blunted BamHI sites to give pDMS197. The BamHI site near the MCS was regenerated after cloning; the BamHI site near the 5' end of TcR/TetR was not.
The resulting plasmid together with others in this deposited series contain the conditional R6K ori, the origin of transfer (oriT) which allows conjugative transfer from permissive hosts, the sacB1 gene to provide negative selection and a MCS, along with a range of different AbR markers. Moreover, the use of Tc also allows an alternative negative selection using fusaric acid and chlortetracycline as described previously (Maloy and Nunn, 1981).
Proper citation: RRID:Addgene_43831 Copy
Vector Backbone Description: Backbone Size:3488; Vector Backbone:pACYC184; Vector Types:Synthetic Biology; Bacterial Resistance:Tetracycline
Defining Citation: PMID:30462270
Comments: Accession Numer MG649421, Alternative ID = pGT402 . Please visit https://www.biorxiv.org/content/early/2018/07/04/361626 for bioRxiv preprint.
Proper citation: RRID:Addgene_114163 Copy
Vector Backbone Description: Backbone Size:3488; Vector Backbone:pACYC184; Vector Types:Synthetic Biology; Bacterial Resistance:Tetracycline
Defining Citation: PMID:30462270
Comments: Accession Number MG649428 , Alternative ID = pGT407 . Please visit https://www.biorxiv.org/content/early/2018/07/04/361626 for bioRxiv preprint.
Proper citation: RRID:Addgene_114168 Copy
Vector Backbone Description: Backbone Size:3488; Vector Backbone:pACYC184; Vector Types:Synthetic Biology; Bacterial Resistance:Tetracycline
Defining Citation: PMID:30462270
Comments: Accession Number MG649423, Alternative ID = pGT404 . Please visit https://www.biorxiv.org/content/early/2018/07/04/361626 for bioRxiv preprint.
Proper citation: RRID:Addgene_114165 Copy
Vector Backbone Description: Backbone Size:3488; Vector Backbone:pACYC184; Vector Types:Synthetic Biology; Bacterial Resistance:Tetracycline
Defining Citation: PMID:30462270
Comments: Accession Number MG649427, Alternative ID = pGT408 . Please visit https://www.biorxiv.org/content/early/2018/07/04/361626 for bioRxiv preprint.
Proper citation: RRID:Addgene_114169 Copy
Vector Backbone Description: Backbone Size:3488; Vector Backbone:pACYC184; Vector Types:Synthetic Biology; Bacterial Resistance:Tetracycline
Defining Citation: PMID:30462270
Comments: Accession Number MG649429, Alternative ID = pGT409 . Please visit https://www.biorxiv.org/content/early/2018/07/04/361626 for bioRxiv preprint.
Proper citation: RRID:Addgene_114170 Copy
Vector Backbone Description: Vector Backbone:NA; Vector Types:Bacterial Expression; Bacterial Resistance:Tetracycline
Defining Citation: PMID:39919271
Proper citation: RRID:Addgene_231325 Copy
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