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  • Organism:saccharomyces cerevisiae (facet)


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Plasmid Name Proper Citation Insert Name Organism Bacterial Resistance Defining Citation Comments Vector Backbone Description Relevant Mutation Record Last Update Mentions Count
plIVMD217
 
Resource Report
Resource Website
RRID:Addgene_160939 MCP-mTagBFP2-HA Tag Saccharomyces cerevisiae Ampicillin PMID:33037152 Vector Backbone:psh100; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:08:46 0
pSMH1741
 
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RRID:Addgene_160930 HHF1 Saccharomyces cerevisiae Ampicillin This is an improved version of the plasmid reported here: https://www.sciencedirect.com/science/article/abs/pii/S096921261930440X https://doi.org/10.1016/j.str.2019.12.002 All histone genes code for S. cerevisiae proteins. Expression and purification procedures are as described. Backbone Marker:Berkeley Macrolab; Backbone Size:4786; Vector Backbone:pET (https://www.addgene.org/29666/); Vector Types:; Bacterial Resistance:Ampicillin codon-optimized for E. coli -- see provided sequence 2026-08-15 01:08:46 0
pSMH1742
 
Resource Report
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RRID:Addgene_160929 HHF1 Saccharomyces cerevisiae Ampicillin This is an improved version of the plasmid reported here: https://www.sciencedirect.com/science/article/abs/pii/S096921261930440X https://doi.org/10.1016/j.str.2019.12.002 All histone genes code for S. cerevisiae proteins. Backbone Marker:Berkeley Macrolab; Backbone Size:4786; Vector Backbone:pET (https://www.addgene.org/29666/); Vector Types:; Bacterial Resistance:Ampicillin codon-optimized for E. coli -- see provided sequence 2026-08-15 01:08:49 0
pGST-pol1-N1-2A2
 
Resource Report
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RRID:Addgene_160990 pol1 Saccharomyces cerevisiae Ampicillin PMID:32923642 Vector Backbone:pGST-parallel; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin F58A/D62A 2026-08-15 01:08:46 0
pGST-pol1-N1
 
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Resource Website
RRID:Addgene_160988 pol1 Saccharomyces cerevisiae Ampicillin PMID:32923642 Vector Backbone:pGST-parallel; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:08:46 0
Ingolia lab S cerevisiae CRISPRi v1 – barcodes 181005
 
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Resource Website
RRID:Addgene_161769 gRNA library targeting yeast genes Saccharomyces cerevisiae Ampicillin PMID:33757429 Backbone Marker:Addgene 35452; Vector Backbone:pRS41x; Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Ampicillin 2026-08-15 01:08:57 0
p406TEF1
 
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RRID:Addgene_15976 TEF1 promoter + polylinker + CYC1 terminator Saccharomyces cerevisiae Ampicillin Mumberg D, Muller R, Funk M. Gene 156:119-22 (1995). Backbone Marker:Sikorski & Hieter, Genetics 122:19-27 (1989); Backbone Size:4283; Vector Backbone:pRS406; Vector Types:Bacterial Expression, Yeast Expression; Bacterial Resistance:Ampicillin TEF1 promoter is between SacI and XbaI restriction sites. 2026-08-15 01:08:33 0
p406CYC1
 
Resource Report
Resource Website
RRID:Addgene_15975 CYC1 promoter + polylinker + CYC1 terminator Saccharomyces cerevisiae Ampicillin Mumberg D, Muller R, Funk M. Gene 156:119-22 (1995). Backbone Marker:Sikorski & Hieter, Genetics 122:19-27 (1989); Backbone Size:4283; Vector Backbone:pRS406; Vector Types:Bacterial Expression, Yeast Expression; Bacterial Resistance:Ampicillin CYC1 promoter is between SacI and XbaI restriction sites. 2026-08-15 01:08:31 0
p406ADH1
 
Resource Report
Resource Website
1+ mentions
RRID:Addgene_15974 ADH1 promoter + polylinker + CYC1 terminator Saccharomyces cerevisiae Ampicillin Mumberg D, Muller R, Funk M. Gene 156:119-22 (1995). Backbone Marker:Sikorski & Hieter, Genetics 122:19-27 (1989); Backbone Size:4283; Vector Backbone:pRS406; Vector Types:Bacterial Expression, Yeast Expression; Bacterial Resistance:Ampicillin ADH1 promoter is between SacI and XbaI restriction sites. 2026-08-15 01:08:31 2
p404TDH3
 
Resource Report
Resource Website
RRID:Addgene_15973 TDH3 promoter + polylinker + CYC1 terminator Saccharomyces cerevisiae Ampicillin Mumberg D, Muller R, Funk M. Gene 156:119-22 (1995). Backbone Marker:Sikorski & Hieter, Genetics 122:19-27 (1989); Backbone Size:4173; Vector Backbone:pRS404; Vector Types:Bacterial Expression, Yeast Expression; Bacterial Resistance:Ampicillin TDH3 promoter is between SacI and XbaI restriction sites. 2026-08-15 01:08:31 0
pBAC690-TEF
 
Resource Report
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RRID:Addgene_160432 S. cerevisiae TEF2 promoter Saccharomyces cerevisiae Ampicillin PMID:33311538 Please visit https://www.biorxiv.org/content/10.1101/2020.05.11.089300v1 for bioRxiv preprint. Parent vector is described in "Optimized cassettes for fluorescent protein tagging in Saccharomyces cerevisiae", Mark A Sheff , Kurt S ThornYeast 2004 Jun;21(8):661-70. doi: 10.1002/yea.1130. The parent vector was intended for GFP tagging of yeast proteins. We placed the TEF2 promoter directly upstream of GFP for sugar-insensitive expression of GFP under galactose or dextrose conditions. Backbone Size:4894; Vector Backbone:pFA6a–link–yEGFP–Kan; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:08:39 0
pUPD2 CUP2 (GB2716)
 
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RRID:Addgene_160599 CUP2 Saccharomyces cerevisiae Chloramphenicol Compatible with GoldenBraid; insert can be released with BsaI Backbone Marker:self-made; derived from the BioBrick assembly plasmid pSB1C3 ; Vector Backbone:pUPD2; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol BsaI and BsmBI sites removed 2026-08-15 01:08:44 0
pGEX-2T-RNAPII-CTD wt #14-HA
 
Resource Report
Resource Website
1+ mentions
RRID:Addgene_160688 RNA Polymerase II CTD Saccharomyces cerevisiae Ampicillin PMID:9468530 Vector Backbone:pGEX-2T; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin C terminal domain only 2026-08-15 01:08:46 1
pcDNA3.1 CIM5 (Rpt-1) GFP
 
Resource Report
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RRID:Addgene_16074 CIM5 Saccharomyces cerevisiae Ampicillin PMID:16810255 Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:08:46 0
pJJB170
 
Resource Report
Resource Website
RRID:Addgene_158600 ADR1c Saccharomyces cerevisiae Kanamycin PMID:33046851 Vector Backbone:Custom; Vector Types:Yeast Expression; Bacterial Resistance:Kanamycin 2026-08-15 01:08:18 0
yHS1
 
Resource Report
Resource Website
RRID:Addgene_159150 yHS1 Saccharomyces cerevisiae Ampicillin PMID:27247412 The first-generation heme sensor, HS1, consists of a heme-binding domain, the His/Met coordinating 4-alpha-helical bundle hemoprotein cytochrome b562 (Cyt b562) (13), fused to a pair of fluorescent proteins, EGFP and Katushka 2 (mKATE2) Backbone Marker:M. Funk; Backbone Size:6900; Vector Backbone:p415GPD; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:08:25 0
nuc-yHS1-M7A
 
Resource Report
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RRID:Addgene_159167 nuc-yHS1-M7A Saccharomyces cerevisiae Ampicillin PMID:27247412 The first-generation heme sensor, HS1, consists of a heme-binding domain, the His/Met coordinating 4-alpha-helical bundle hemoprotein cytochrome b562 (Cyt b562) (13), fused to a pair of fluorescent proteins, EGFP and Katushka 2 (mKATE2) Backbone Marker:M. Funk; Backbone Size:6900; Vector Backbone:p415GPD; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin Mutated Met 7 of the cyt b562 module of nuc-yHS1 to Ala (M7A), creates medium affinity heme sensor 2026-08-15 01:08:27 0
pJO818 (pRS425 Gal1-Ufd2-flag)
 
Resource Report
Resource Website
RRID:Addgene_159522 Ufd2 Saccharomyces cerevisiae Ampicillin Backbone Size:7540; Vector Backbone:pRS425-Gal1; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:08:28 0
p405GALL
 
Resource Report
Resource Website
RRID:Addgene_15964 p405GALL Saccharomyces cerevisiae Ampicillin Mumberg D, Muller R, Funk M. Nucleic Acids Res. 25:5767-8 (1994). Backbone Marker:Sikorski & Hieter, Genetics 122:19-27 (1989); Backbone Size:3814; Vector Backbone:pRS405; Vector Types:Bacterial Expression, Yeast Expression; Bacterial Resistance:Ampicillin GALL promoter is between SacI and XbaI restriction sites. 2026-08-15 01:08:32 0
pCSJ392
 
Resource Report
Resource Website
RRID:Addgene_159722 GID10 Saccharomyces cerevisiae Ampicillin PMID:31337681 Vector Backbone:pGADCg; Vector Types:Yeast Expression, Yeast two-hybrid assay; Bacterial Resistance:Ampicillin remove stop codon 2026-08-15 01:08:33 0

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