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Species: Saccharomyces cerevisiae
Genetic Insert: MCP-mTagBFP2-HA Tag
Vector Backbone Description: Vector Backbone:psh100; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33037152
Proper citation: RRID:Addgene_160939 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: HHF1
Vector Backbone Description: Backbone Marker:Berkeley Macrolab; Backbone Size:4786; Vector Backbone:pET (https://www.addgene.org/29666/); Vector Types:; Bacterial Resistance:Ampicillin
Comments: This is an improved version of the plasmid reported here:
https://www.sciencedirect.com/science/article/abs/pii/S096921261930440X
https://doi.org/10.1016/j.str.2019.12.002
All histone genes code for S. cerevisiae proteins. Expression and purification procedures are as described.
Proper citation: RRID:Addgene_160930 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: HHF1
Vector Backbone Description: Backbone Marker:Berkeley Macrolab; Backbone Size:4786; Vector Backbone:pET (https://www.addgene.org/29666/); Vector Types:; Bacterial Resistance:Ampicillin
Comments: This is an improved version of the plasmid reported here:
https://www.sciencedirect.com/science/article/abs/pii/S096921261930440X
https://doi.org/10.1016/j.str.2019.12.002
All histone genes code for S. cerevisiae proteins.
Proper citation: RRID:Addgene_160929 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: pol1
Vector Backbone Description: Vector Backbone:pGST-parallel; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32923642
Proper citation: RRID:Addgene_160990 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: pol1
Vector Backbone Description: Vector Backbone:pGST-parallel; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32923642
Proper citation: RRID:Addgene_160988 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: gRNA library targeting yeast genes
Vector Backbone Description: Backbone Marker:Addgene 35452; Vector Backbone:pRS41x; Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33757429
Proper citation: RRID:Addgene_161769 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: TEF1 promoter + polylinker + CYC1 terminator
Vector Backbone Description: Backbone Marker:Sikorski & Hieter, Genetics 122:19-27 (1989); Backbone Size:4283; Vector Backbone:pRS406; Vector Types:Bacterial Expression, Yeast Expression; Bacterial Resistance:Ampicillin
Comments: Mumberg D, Muller R, Funk M. Gene 156:119-22 (1995).
Proper citation: RRID:Addgene_15976 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: CYC1 promoter + polylinker + CYC1 terminator
Vector Backbone Description: Backbone Marker:Sikorski & Hieter, Genetics 122:19-27 (1989); Backbone Size:4283; Vector Backbone:pRS406; Vector Types:Bacterial Expression, Yeast Expression; Bacterial Resistance:Ampicillin
Comments: Mumberg D, Muller R, Funk M. Gene 156:119-22 (1995).
Proper citation: RRID:Addgene_15975 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: ADH1 promoter + polylinker + CYC1 terminator
Vector Backbone Description: Backbone Marker:Sikorski & Hieter, Genetics 122:19-27 (1989); Backbone Size:4283; Vector Backbone:pRS406; Vector Types:Bacterial Expression, Yeast Expression; Bacterial Resistance:Ampicillin
Comments: Mumberg D, Muller R, Funk M. Gene 156:119-22 (1995).
Proper citation: RRID:Addgene_15974 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: TDH3 promoter + polylinker + CYC1 terminator
Vector Backbone Description: Backbone Marker:Sikorski & Hieter, Genetics 122:19-27 (1989); Backbone Size:4173; Vector Backbone:pRS404; Vector Types:Bacterial Expression, Yeast Expression; Bacterial Resistance:Ampicillin
Comments: Mumberg D, Muller R, Funk M. Gene 156:119-22 (1995).
Proper citation: RRID:Addgene_15973 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: S. cerevisiae TEF2 promoter
Vector Backbone Description: Backbone Size:4894; Vector Backbone:pFA6a–link–yEGFP–Kan; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33311538
Comments: Please visit https://www.biorxiv.org/content/10.1101/2020.05.11.089300v1 for bioRxiv preprint.
Parent vector is described in "Optimized cassettes for fluorescent protein tagging in Saccharomyces cerevisiae", Mark A Sheff , Kurt S ThornYeast 2004 Jun;21(8):661-70. doi: 10.1002/yea.1130. The parent vector was intended for GFP tagging of yeast proteins. We placed the TEF2 promoter directly upstream of GFP for sugar-insensitive expression of GFP under galactose or dextrose conditions.
Proper citation: RRID:Addgene_160432 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: CUP2
Vector Backbone Description: Backbone Marker:self-made; derived from the BioBrick assembly plasmid pSB1C3 ; Vector Backbone:pUPD2; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
Comments: Compatible with GoldenBraid; insert can be released with BsaI
Proper citation: RRID:Addgene_160599 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: RNA Polymerase II CTD
Vector Backbone Description: Vector Backbone:pGEX-2T; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9468530
Proper citation: RRID:Addgene_160688 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: CIM5
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16810255
Proper citation: RRID:Addgene_16074 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: ADR1c
Vector Backbone Description: Vector Backbone:Custom; Vector Types:Yeast Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33046851
Proper citation: RRID:Addgene_158600 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: yHS1
Vector Backbone Description: Backbone Marker:M. Funk; Backbone Size:6900; Vector Backbone:p415GPD; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27247412
Comments: The first-generation heme sensor, HS1, consists of a heme-binding domain, the His/Met coordinating 4-alpha-helical bundle hemoprotein cytochrome b562 (Cyt b562) (13), fused to a pair of fluorescent proteins, EGFP and Katushka 2 (mKATE2)
Proper citation: RRID:Addgene_159150 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: nuc-yHS1-M7A
Vector Backbone Description: Backbone Marker:M. Funk; Backbone Size:6900; Vector Backbone:p415GPD; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27247412
Comments: The first-generation heme sensor, HS1, consists of a heme-binding domain, the His/Met coordinating 4-alpha-helical bundle hemoprotein cytochrome b562 (Cyt b562) (13), fused to a pair of fluorescent proteins, EGFP and Katushka 2 (mKATE2)
Proper citation: RRID:Addgene_159167 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Ufd2
Vector Backbone Description: Backbone Size:7540; Vector Backbone:pRS425-Gal1; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_159522 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: p405GALL
Vector Backbone Description: Backbone Marker:Sikorski & Hieter, Genetics 122:19-27 (1989); Backbone Size:3814; Vector Backbone:pRS405; Vector Types:Bacterial Expression, Yeast Expression; Bacterial Resistance:Ampicillin
Comments: Mumberg D, Muller R, Funk M. Nucleic Acids Res. 25:5767-8 (1994).
Proper citation: RRID:Addgene_15964 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: GID10
Vector Backbone Description: Vector Backbone:pGADCg; Vector Types:Yeast Expression, Yeast two-hybrid assay; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31337681
Proper citation: RRID:Addgene_159722 Copy
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