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Species: Calendula officinalis
Genetic Insert: 35Sshort_TMV_CYP716A393_35S
Vector Backbone Description: Vector Backbone:pICH47742; Vector Types:Plant Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:40721615
Proper citation: RRID:Addgene_227544 Copy
Species: Calendula officinalis
Genetic Insert: 35Sshort_TMV_CYP716A429_35S
Vector Backbone Description: Vector Backbone:pICH47742; Vector Types:Plant Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:40721615
Proper citation: RRID:Addgene_227545 Copy
Species: Homo sapiens
Genetic Insert: GAC isoform of F7
Vector Backbone Description: Vector Backbone:pGEX; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38898113
Proper citation: RRID:Addgene_227829 Copy
Species: Homo sapiens
Genetic Insert: WEE2
Vector Backbone Description: Backbone Marker:PEL; Backbone Size:9019; Vector Backbone:pDest663; Vector Types:Mammalian Expression, Lentiviral, Gateway Destination; Bacterial Resistance:Ampicillin
Comments: These plasmids were generated as part of the Illuminating the Druggable Genome (IDG) program sponsored by the NIH Common Fund. The goal of this program is to identify, gather, and distribute information and resources for proteins that currently are not well-studied yet belong to commonly drug-targeted protein families: protein kinases, non-olfactory G-protein coupled receptors (GPCRs), and ion channels. The IDG program is designed to develop fundamental research tools for understudied proteins, elucidate their function, and disseminate the IDG-related resources and data to the greater scientific community. These lentiviral gateway destination plasmids were generated, as part of the Kinase-Data and Resource Generating Center (DRGC), using the single fragment or multisite gateway cloning technology. They were used for generating proteomics-based protein-interaction and protein-proximity networks that can be accessed through the kinase-DRGC website https://darkkinome.org/. Each plasmid has either an N- or C-terminal fusion protein (Flag/ V5/V5-miniTurbo/V5-TurboID/miniTurbo-V5/TurboID-V5) tagged understudied kinase driven under either a CMV or Ubiquitin (UBC) promoter. All inserts in the pENTR plasmids used to generate the deposited destination plasmids were end-sequenced and insert size validated prior to multi-site gateway cloning. The combined insert size of the single fragment or three fragment destination plasmids were confirmed by restriction digestion (BsrGI, and EcoRV for pDest667; BsrGI, EcoRV, and BstZ17I for pDest663; and BsrGI for pHAGE) and all plasmids were partially sequenced to ensure in-frame ORFs and fusion tags.
Proper citation: RRID:Addgene_189895 Copy
Species: Other
Genetic Insert: tTA
Vector Backbone Description: Backbone Size:4842; Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_191210 Copy
Species: S. purpuratus
Genetic Insert: AGS-dGoLoco1
Vector Backbone Description: Vector Backbone:PSP64; Vector Types:In vitro transcription (mRNA synthesis); Bacterial Resistance:Ampicillin
Defining Citation: PMID:31439829
Proper citation: RRID:Addgene_185577 Copy
Species: Homo sapiens
Genetic Insert: DCLK3
Vector Backbone Description: Backbone Marker:PEL; Backbone Size:9019; Vector Backbone:pDest663; Vector Types:Mammalian Expression, Lentiviral, Gateway Destination; Bacterial Resistance:Ampicillin
Comments: These plasmids were generated as part of the Illuminating the Druggable Genome (IDG) program sponsored by the NIH Common Fund. The goal of this program is to identify, gather, and distribute information and resources for proteins that currently are not well-studied yet belong to commonly drug-targeted protein families: protein kinases, non-olfactory G-protein coupled receptors (GPCRs), and ion channels. The IDG program is designed to develop fundamental research tools for understudied proteins, elucidate their function, and disseminate the IDG-related resources and data to the greater scientific community. These lentiviral gateway destination plasmids were generated, as part of the Kinase-Data and Resource Generating Center (DRGC), using the single fragment or multisite gateway cloning technology. They were used for generating proteomics-based protein-interaction and protein-proximity networks that can be accessed through the kinase-DRGC website https://darkkinome.org/. Each plasmid has either an N- or C-terminal fusion protein (Flag/ V5/V5-miniTurbo/V5-TurboID/miniTurbo-V5/TurboID-V5) tagged understudied kinase driven under either a CMV or Ubiquitin (UBC) promoter. All inserts in the pENTR plasmids used to generate the deposited destination plasmids were end-sequenced and insert size validated prior to multi-site gateway cloning. The combined insert size of the single fragment or three fragment destination plasmids were confirmed by restriction digestion (BsrGI, and EcoRV for pDest667; BsrGI, EcoRV, and BstZ17I for pDest663; and BsrGI for pHAGE) and all plasmids were partially sequenced to ensure in-frame ORFs and fusion tags.
Proper citation: RRID:Addgene_189891 Copy
Species: Synthetic
Genetic Insert: CR3 constant region - hU6 sgRNA promoter flanked by BsmBI sites
Vector Backbone Description: Vector Backbone:pUCIDT; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36576240
Comments: Please visit https://doi.org/10.1101/2022.07.13.499814 for bioRxiv preprint.
Proper citation: RRID:Addgene_187239 Copy
Species: Mus musculus
Genetic Insert: gRNA targeting Cdkn2c
Vector Backbone Description: Vector Backbone:pLL3.3; Vector Types:Lentiviral, Cre/Lox, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33608386
Proper citation: RRID:Addgene_173584 Copy
Species: Synthetic
Genetic Insert: Lentiviral sgRNA vector for Perturb-seq with mU6 promoter
Vector Backbone Description: Vector Backbone:pBA904; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36576240
Comments: Please visit https://doi.org/10.1101/2022.07.13.499814 for bioRxiv preprint.
Proper citation: RRID:Addgene_187240 Copy
Species: S. purpuratus
Genetic Insert: Vasa-C3
Vector Backbone Description: Vector Backbone:PSP64; Vector Types:In vitro transcription (mRNA synthesis); Bacterial Resistance:Ampicillin
Defining Citation: PMID:31515896
Proper citation: RRID:Addgene_185587 Copy
Species: S. purpuratus
Genetic Insert: Vasa-S83K
Vector Backbone Description: Vector Backbone:PSP64; Vector Types:In vitro transcription (mRNA synthesis); Bacterial Resistance:Ampicillin
Defining Citation: PMID:31515896
Proper citation: RRID:Addgene_185588 Copy
Species: S. purpuratus
Genetic Insert: Vasa
Vector Backbone Description: Vector Backbone:PSP64; Vector Types:In vitro transcription (mRNA synthesis); Bacterial Resistance:Ampicillin
Defining Citation: PMID:31515896
Proper citation: RRID:Addgene_185585 Copy
Species: Homo sapiens
Genetic Insert: Hero9
Vector Backbone Description: Backbone Marker:Takara; Vector Backbone:pCold I; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32163402
Comments: Also refer to Morimoto et al. (2022) "Fusion with heat-resistant obscure (Hero) proteins have the potential to improve the molecular property of recombinant proteins" bioRxiv. https://doi.org/10.1101/2022.03.03.482928
Proper citation: RRID:Addgene_187927 Copy
Species: Homo sapiens
Genetic Insert: Hero7
Vector Backbone Description: Backbone Marker:Takara; Vector Backbone:pCold I; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32163402
Comments: Also refer to Morimoto et al. (2022) "Fusion with heat-resistant obscure (Hero) proteins have the potential to improve the molecular property of recombinant proteins" bioRxiv. https://doi.org/10.1101/2022.03.03.482928
Proper citation: RRID:Addgene_187926 Copy
Species: Homo sapiens
Genetic Insert: APOE
Vector Backbone Description: Backbone Size:9205; Vector Backbone:FUGW; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Comments: E4: rs429358-C (C112R) and rs7412-C
Proper citation: RRID:Addgene_190809 Copy
Species: Other
Genetic Insert: Influenza B Nucleoprotein
Vector Backbone Description: Backbone Size:5446; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35694292
Proper citation: RRID:Addgene_187921 Copy
Species: Homo sapiens
Genetic Insert: Hero13
Vector Backbone Description: Backbone Marker:Takara; Vector Backbone:pCold I; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32163402
Comments: Also refer to Morimoto et al. (2022) "Fusion with heat-resistant obscure (Hero) proteins have the potential to improve the molecular property of recombinant proteins" bioRxiv. https://doi.org/10.1101/2022.03.03.482928
Proper citation: RRID:Addgene_187929 Copy
Species: Homo sapiens
Genetic Insert: NEK1
Vector Backbone Description: Backbone Marker:PEL; Backbone Size:9019; Vector Backbone:pDest663; Vector Types:Mammalian Expression, Lentiviral, Gateway Destination; Bacterial Resistance:Ampicillin
Comments: These plasmids were generated as part of the Illuminating the Druggable Genome (IDG) program sponsored by the NIH Common Fund. The goal of this program is to identify, gather, and distribute information and resources for proteins that currently are not well-studied yet belong to commonly drug-targeted protein families: protein kinases, non-olfactory G-protein coupled receptors (GPCRs), and ion channels. The IDG program is designed to develop fundamental research tools for understudied proteins, elucidate their function, and disseminate the IDG-related resources and data to the greater scientific community. These lentiviral gateway destination plasmids were generated, as part of the Kinase-Data and Resource Generating Center (DRGC), using the single fragment or multisite gateway cloning technology. They were used for generating proteomics-based protein-interaction and protein-proximity networks that can be accessed through the kinase-DRGC website https://darkkinome.org/. Each plasmid has either an N- or C-terminal fusion protein (Flag/ V5/V5-miniTurbo/V5-TurboID/miniTurbo-V5/TurboID-V5) tagged understudied kinase driven under either a CMV or Ubiquitin (UBC) promoter. All inserts in the pENTR plasmids used to generate the deposited destination plasmids were end-sequenced and insert size validated prior to multi-site gateway cloning. The combined insert size of the single fragment or three fragment destination plasmids were confirmed by restriction digestion (BsrGI, and EcoRV for pDest667; BsrGI, EcoRV, and BstZ17I for pDest663; and BsrGI for pHAGE) and all plasmids were partially sequenced to ensure in-frame ORFs and fusion tags.
Proper citation: RRID:Addgene_189871 Copy
Species: Neonothopanus nambi
Genetic Insert: h3h
Vector Backbone Description: Backbone Marker:Adamala Lab; Backbone Size:2918; Vector Backbone:pCI-T7Max-UTR1-CTerminus8xHis-T500; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35798760
Proper citation: RRID:Addgene_188303 Copy
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