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Vector Backbone Description: Backbone Marker:Novagen, EMBL; Backbone Size:5737; Vector Backbone:pET, pETM14; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:23410102
Comments: Test each preparation of the plasmid by transforming it into non-resistant cells (ex. DH5a) to ensure negative selection by ccdB kills all the transformed cells as expected.
Proper citation: RRID:Addgene_43974 Copy
Vector Backbone Description: Backbone Marker:http://www.arabidopsis.org/servlet/TairObject?id=500300075&type=vector; Backbone Size:11405; Vector Backbone:pFGC5941; Vector Types:; Bacterial Resistance:Kanamycin
Defining Citation: PMID:23098881
Proper citation: RRID:Addgene_44020 Copy
Genetic Insert: puroR
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2670; Vector Backbone:pDONR221-P4rP3r; Vector Types:; Bacterial Resistance:Kanamycin
Defining Citation: PMID:23637962
Proper citation: RRID:Addgene_43965 Copy
Genetic Insert: pA
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2557; Vector Backbone:pDONR221-P3P2; Vector Types:Multisite Gateway ENTR clone; Bacterial Resistance:Kanamycin
Defining Citation: PMID:23637962
Proper citation: RRID:Addgene_43966 Copy
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5827; Vector Backbone:pET, pCoofy18; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:23410102
Comments: Test each preparation of the plasmid by transforming it into non-resistant cells (ex. DH5a) to ensure negative selection by ccdB kills all the transformed cells as expected.
Proper citation: RRID:Addgene_44001 Copy
Vector Backbone Description: Backbone Marker:Novagen, EMBL; Backbone Size:6657; Vector Backbone:pET, pET28M-Sumo3; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:23410102
Comments: Test each preparation of the plasmid by transforming it into non-resistant cells (ex. DH5a) to ensure negative selection by ccdB kills all the transformed cells as expected.
Proper citation: RRID:Addgene_43990 Copy
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:6669; Vector Backbone:pET, pCoofy6; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:23410102
Comments: Test each preparation of the plasmid by transforming it into non-resistant cells (ex. DH5a) to ensure negative selection by ccdB kills all the transformed cells as expected.
Proper citation: RRID:Addgene_43991 Copy
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5764; Vector Backbone:pET, pCoofy7; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:23410102
Comments: Test each preparation of the plasmid by transforming it into non-resistant cells (ex. DH5a) to ensure negative selection by ccdB kills all the transformed cells as expected.
Proper citation: RRID:Addgene_43992 Copy
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5808; Vector Backbone:pET, pCoofy12; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:23410102
Comments: C-terminal Tag has 6 His residues, LP2 primer needs to be adapted to 6His
Test each preparation of the plasmid by transforming it into non-resistant cells (ex. DH5a) to ensure negative selection by ccdB kills all the transformed cells as expected.
Proper citation: RRID:Addgene_43993 Copy
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5845; Vector Backbone:pET, pCoofy34; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:23410102
Comments: Test each preparation of the plasmid by transforming it into non-resistant cells (ex. DH5a) to ensure negative selection by ccdB kills all the transformed cells as expected.
Proper citation: RRID:Addgene_43994 Copy
Vector Backbone Description: Backbone Marker:Novagen, EMBL; Backbone Size:6070; Vector Backbone:pET, pCoofy14; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:23410102
Comments: Test each preparation of the plasmid by transforming it into non-resistant cells (ex. DH5a) to ensure negative selection by ccdB kills all the transformed cells as expected.
Proper citation: RRID:Addgene_43996 Copy
Vector Backbone Description: Backbone Marker:http://www.cambia.org/daisy/cambia/2045/version/1/part/4/data/pCAMBIA1300.pdf?branch=main&language=default; Backbone Size:8958; Vector Backbone:pCambia1300; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:23098881
Comments: Note- the SpeI site located within the Kanamycin resistance cassette was destroyed (tested by digest). The fact that the plasmid is growing on Kanamycin indicates that the loss of the SpeI site does not affect the plasmid's function.
Proper citation: RRID:Addgene_44183 Copy
Species: Synthetic
Genetic Insert: TagRFP675
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:5072; Vector Backbone:pN1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:23677204
Proper citation: RRID:Addgene_44277 Copy
Species: Synthetic
Genetic Insert: TagRFP675
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pN1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:23677204
Proper citation: RRID:Addgene_44279 Copy
Species: Homo sapiens
Genetic Insert: Pyruvate Kinase M2
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5400; Vector Backbone:pET-28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:18337815
Proper citation: RRID:Addgene_44242 Copy
Species: Homo sapiens
Genetic Insert: Pyruvate Kinase M1
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5400; Vector Backbone:pET-28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:18337815
Proper citation: RRID:Addgene_44241 Copy
Species: Homo sapiens
Genetic Insert: NIPP1
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEYFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:11739654
Proper citation: RRID:Addgene_44229 Copy
Species: Homo sapiens
Genetic Insert: RepoMan
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:16492807
Proper citation: RRID:Addgene_44213 Copy
Vector Backbone Description: Backbone Size:4850; Vector Backbone:pBabe; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Kanamycin
Defining Citation: PMID:15644491
Proper citation: RRID:Addgene_44433 Copy
Genetic Insert: GFP
Vector Backbone Description: Backbone Size:2048; Vector Backbone:pZE; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:22326628
Proper citation: RRID:Addgene_44381 Copy
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