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Species: Synthetic
Genetic Insert: pHRed
Vector Backbone Description: Backbone Size:9200; Vector Backbone:FUGW; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24096541
Proper citation: RRID:Addgene_65742 Copy
Species: Homo sapiens
Genetic Insert: ELAVL3
Vector Backbone Description: Backbone Marker:Life Technologies, Modified by Tuschl lab; Backbone Size:5372; Vector Backbone:pcDNA5/FRT; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_65756 Copy
Species: Homo sapiens
Genetic Insert: ELAVL2
Vector Backbone Description: Backbone Marker:Life Technologies, Modified by Tuschl lab; Vector Backbone:pcDNA5/FRT; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_65758 Copy
Species: Homo sapiens
Genetic Insert: ELAVL4
Vector Backbone Description: Backbone Marker:Life Technologies, Modified by Tuschl lab; Backbone Size:5372; Vector Backbone:pcDNA5/FRT; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_65757 Copy
Species: Other
Genetic Insert: mammalian codon-optimized Streptococcus thermophilus1 Cas9-NLS, and St1Cas9 gRNA
Vector Backbone Description: Backbone Marker:EMD Millipore; Vector Backbone:pACYCDuet-1; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:26098369
Comments: Use BspMI sites to insert sgRNA spacer sequence into the vector.
Proper citation: RRID:Addgene_65769 Copy
Species: Other
Genetic Insert: mammalian codon-optimized Streptococcus pyogenes dCas9 (D10A/H840A)-NLS-3XFlag, and SpCas9 gRNA
Vector Backbone Description: Backbone Marker:EMD Millipore; Vector Backbone:pACYCDuet-1; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:26098369
Comments: Use BsaI sites to insert sgRNA spacer sequence into the vector.
Proper citation: RRID:Addgene_65768 Copy
Species: Homo sapiens
Genetic Insert: eRF3a
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:4572; Vector Backbone:pCI-neo; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25147240
Proper citation: RRID:Addgene_65811 Copy
Species: Synthetic
Genetic Insert: SpCas9 gRNA backbone, without spacer sequence
Vector Backbone Description: Backbone Marker:Joung lab (Addgene Plasmid # 43860); Vector Backbone:MLM3636; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26098369
Comments: Use BsmBI sites to insert sgRNA spacer sequence into the vector. See the supplemental document "Joung Lab gRNA Cloning Protocol" for more details.
This plasmid has been found to be somewhat unstable and prone to concatenation. Concatenation often does not impact plasmid function, but may reduce transformation efficiencies. You may need to screen multiple colonies to isolate the monomeric version of this plasmid. If you still have trouble isolating the monomeric version, you might consider linearizing, gel extracting, re-ligating, and transforming the plasmid.
Proper citation: RRID:Addgene_65777 Copy
Species: Synthetic
Genetic Insert: SaCas9 gRNA backbone, without spacer sequence
Vector Backbone Description: Backbone Marker:Joung lab (Addgene Plasmid # 43860); Vector Backbone:MLM3636; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26098369
Comments: Use BsmBI sites to insert sgRNA spacer sequence into the vector. See the supplemental document "Joung Lab gRNA Cloning Protocol" for more details.
UPDATE 5/31/2016: The Joung Lab recommends using BPK2660 (Addgene plasmid # 70709) instead of VVT1 as an SaCas9 guide RNA cloning vector, as guide RNAs cloned into BPK2660 are more effective than those cloned into VVT1. The difference in efficacy is due to VVT1 containing the full length guide RNA (predicted from the natural CRISPR sequence in S. aureus), whereas BPK2660 uses a truncated gRNA that is more effective. For the same oligo/spacer, the rate of mutagenesis is greater using BPK2660. See Kleinstiver et al., 2015 Nat Biotech for additional information on the truncated gRNA plasmid (https://www.ncbi.nlm.nih.gov/pubmed/26524662).
Proper citation: RRID:Addgene_65779 Copy
Species: Caenorhabditis elegans
Genetic Insert: NLG-1
Vector Backbone Description: Backbone Marker:C.Bargmann; Backbone Size:3500; Vector Backbone:pSM; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18255029
Proper citation: RRID:Addgene_65827 Copy
Species: Homo sapiens
Genetic Insert: RNaseH1
Vector Backbone Description: Backbone Marker:Life Technologies, Modified by Tuschl lab; Vector Backbone:pcDNA5/FRT; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_65783 Copy
Species: Homo sapiens
Genetic Insert: RNaseH1
Vector Backbone Description: Backbone Marker:Life Technologies, Modified by Tuschl lab; Vector Backbone:pcDNA5/FRT; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_65782 Copy
Genetic Insert: MG1655 Z1 malE
Vector Backbone Description: Vector Backbone:n/a; Vector Types:; Bacterial Resistance:None
Defining Citation: PMID:26900850
Comments: F-, lambda-, rph-1, laciq, PN25-tetR, SpR, malE-
Proper citation: RRID:Addgene_65915 Copy
Species: Mus musculus
Genetic Insert: Enhancer of zeste homolog 2
Vector Backbone Description: Backbone Marker:custom; Backbone Size:6743; Vector Backbone:pINTO-NFH; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25311790
Proper citation: RRID:Addgene_65925 Copy
Species: Synthetic
Genetic Insert: V5-human TEV
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5523; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20308068
Proper citation: RRID:Addgene_65800 Copy
Species: Homo sapiens
Genetic Insert: TPI
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:4572; Vector Backbone:pCI-neo; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25310981
Proper citation: RRID:Addgene_65801 Copy
Species: bacteriophage
Genetic Insert: T7-opt
Vector Backbone Description: Backbone Size:4729; Vector Backbone:pCAGGS; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25392218
Proper citation: RRID:Addgene_65974 Copy
Species: Caenorhabditis elegans
Genetic Insert: Chrimson
Vector Backbone Description: Vector Backbone:pUC57-Kan; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:26022242
Comments: This construct was produced through de novo gene synthesis.
Chrimson amino acid sequence was published in: Klapoetke NC, Murata Y, Kim SS, Pulver SR, Birdsey-Benson A, Cho YK, Morimoto TK, Chuong AS, Carpenter EJ, Tian Z, Wang J, Xie Y, Yan Z, Zhang Y, Chow BY, Surek B, Melkonian M, Jayaraman V, Constantine-Paton M, Wong GK, and Boyden ES. 2014. Independent optical excitation of distinct neural populations. Nat. Meth. 11, 338-346.
Proper citation: RRID:Addgene_66101 Copy
Genetic Insert: phi80 integrase
Vector Backbone Description: Vector Backbone:pAH123; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25849635
Proper citation: RRID:Addgene_66077 Copy
Species: Other
Genetic Insert: CYP119
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5639; Vector Backbone:pET-11a; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24924478
Comments: We cloned the CYP119 gene from the cell suspension of S. solfataricus P1 strain (ATCC 35091).
Proper citation: RRID:Addgene_66131 Copy
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