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| Plasmid Name | Proper Citation | Insert Name | Organism | Bacterial Resistance | Defining Citation |
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FUGW-pHRed Resource Report Resource Website 1+ mentions |
RRID:Addgene_65742 | pHRed | Synthetic | Ampicillin | PMID:24096541 | Backbone Size:9200; Vector Backbone:FUGW; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin | 2026-08-15 01:18:21 | 1 | ||
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pFRT-TODestFLAGHA_HuC Resource Report Resource Website 1+ mentions |
RRID:Addgene_65756 | ELAVL3 | Homo sapiens | Ampicillin | Backbone Marker:Life Technologies, Modified by Tuschl lab; Backbone Size:5372; Vector Backbone:pcDNA5/FRT; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:18:21 | 2 | |||
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pFRT-DestFLAGHA_HuB Resource Report Resource Website 1+ mentions |
RRID:Addgene_65758 | ELAVL2 | Homo sapiens | Ampicillin | Backbone Marker:Life Technologies, Modified by Tuschl lab; Vector Backbone:pcDNA5/FRT; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:18:22 | 1 | |||
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pFRT-TODestFLAGHA_HuD Resource Report Resource Website 1+ mentions |
RRID:Addgene_65757 | ELAVL4 | Homo sapiens | Ampicillin | Backbone Marker:Life Technologies, Modified by Tuschl lab; Backbone Size:5372; Vector Backbone:pcDNA5/FRT; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:18:21 | 4 | |||
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MSP1673 Resource Report Resource Website 1+ mentions |
RRID:Addgene_65769 | mammalian codon-optimized Streptococcus thermophilus1 Cas9-NLS, and St1Cas9 gRNA | Other | Chloramphenicol | PMID:26098369 | Use BspMI sites to insert sgRNA spacer sequence into the vector. | Backbone Marker:EMD Millipore; Vector Backbone:pACYCDuet-1; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Chloramphenicol | 2026-08-15 01:18:21 | 2 | |
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MSP712 Resource Report Resource Website 1+ mentions |
RRID:Addgene_65768 | mammalian codon-optimized Streptococcus pyogenes dCas9 (D10A/H840A)-NLS-3XFlag, and SpCas9 gRNA | Other | Chloramphenicol | PMID:26098369 | Use BsaI sites to insert sgRNA spacer sequence into the vector. | Backbone Marker:EMD Millipore; Vector Backbone:pACYCDuet-1; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Chloramphenicol | D10A and H840A mutations in Cas9 | 2026-08-15 01:18:21 | 1 |
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pCI-MS2V5-eRF3a F76a Resource Report Resource Website 1+ mentions |
RRID:Addgene_65811 | eRF3a | Homo sapiens | Ampicillin | PMID:25147240 | Backbone Marker:Promega; Backbone Size:4572; Vector Backbone:pCI-neo; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | F76a | 2026-08-15 01:18:21 | 1 | |
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BPK1520 Resource Report Resource Website 50+ mentions |
RRID:Addgene_65777 | SpCas9 gRNA backbone, without spacer sequence | Synthetic | Ampicillin | PMID:26098369 | Use BsmBI sites to insert sgRNA spacer sequence into the vector. See the supplemental document "Joung Lab gRNA Cloning Protocol" for more details. This plasmid has been found to be somewhat unstable and prone to concatenation. Concatenation often does not impact plasmid function, but may reduce transformation efficiencies. You may need to screen multiple colonies to isolate the monomeric version of this plasmid. If you still have trouble isolating the monomeric version, you might consider linearizing, gel extracting, re-ligating, and transforming the plasmid. | Backbone Marker:Joung lab (Addgene Plasmid # 43860); Vector Backbone:MLM3636; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin | 2026-08-15 01:18:21 | 63 | |
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VVT1 Resource Report Resource Website 1+ mentions |
RRID:Addgene_65779 | SaCas9 gRNA backbone, without spacer sequence | Synthetic | Ampicillin | PMID:26098369 | Use BsmBI sites to insert sgRNA spacer sequence into the vector. See the supplemental document "Joung Lab gRNA Cloning Protocol" for more details. UPDATE 5/31/2016: The Joung Lab recommends using BPK2660 (Addgene plasmid # 70709) instead of VVT1 as an SaCas9 guide RNA cloning vector, as guide RNAs cloned into BPK2660 are more effective than those cloned into VVT1. The difference in efficacy is due to VVT1 containing the full length guide RNA (predicted from the natural CRISPR sequence in S. aureus), whereas BPK2660 uses a truncated gRNA that is more effective. For the same oligo/spacer, the rate of mutagenesis is greater using BPK2660. See Kleinstiver et al., 2015 Nat Biotech for additional information on the truncated gRNA plasmid (https://www.ncbi.nlm.nih.gov/pubmed/26524662). | Backbone Marker:Joung lab (Addgene Plasmid # 43860); Vector Backbone:MLM3636; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin | 2026-08-15 01:18:22 | 1 | |
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Punc-4::NGL-1::spGFP1-10 Resource Report Resource Website 1+ mentions |
RRID:Addgene_65827 | NLG-1 | Caenorhabditis elegans | Ampicillin | PMID:18255029 | Backbone Marker:C.Bargmann; Backbone Size:3500; Vector Backbone:pSM; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:18:23 | 1 | ||
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pFRT-DestFLAGHA_RNAseH1 Resource Report Resource Website 1+ mentions |
RRID:Addgene_65783 | RNaseH1 | Homo sapiens | Ampicillin | Backbone Marker:Life Technologies, Modified by Tuschl lab; Vector Backbone:pcDNA5/FRT; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:18:21 | 3 | |||
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pFRT-TODestFLAGHA_RNAseH1 Resource Report Resource Website 1+ mentions |
RRID:Addgene_65782 | RNaseH1 | Homo sapiens | Ampicillin | Backbone Marker:Life Technologies, Modified by Tuschl lab; Vector Backbone:pcDNA5/FRT; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:18:21 | 4 | |||
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MG1655 Z1 malE Resource Report Resource Website 1+ mentions |
RRID:Addgene_65915 | MG1655 Z1 malE | None | PMID:26900850 | F-, lambda-, rph-1, laciq, PN25-tetR, SpR, malE- | Vector Backbone:n/a; Vector Types:; Bacterial Resistance:None | 2026-08-15 01:18:23 | 1 | ||
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pINTO-NFH::mEzh2 Resource Report Resource Website 1+ mentions |
RRID:Addgene_65925 | Enhancer of zeste homolog 2 | Mus musculus | Ampicillin | PMID:25311790 | Backbone Marker:custom; Backbone Size:6743; Vector Backbone:pINTO-NFH; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:18:22 | 3 | ||
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pcDNA3.1-V5-hTEV Resource Report Resource Website 1+ mentions |
RRID:Addgene_65800 | V5-human TEV | Synthetic | Ampicillin | PMID:20308068 | Backbone Marker:Invitrogen; Backbone Size:5523; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | codon-optimised to express in mammalian cells | 2026-08-15 01:18:21 | 1 | |
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NG0886 pCI-TPI-WT-4H Resource Report Resource Website 1+ mentions |
RRID:Addgene_65801 | TPI | Homo sapiens | Ampicillin | PMID:25310981 | Backbone Marker:Promega; Backbone Size:4572; Vector Backbone:pCI-neo; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:18:23 | 1 | ||
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T7opt in pCAGGS Resource Report Resource Website 10+ mentions |
RRID:Addgene_65974 | T7-opt | bacteriophage | Ampicillin | PMID:25392218 | Backbone Size:4729; Vector Backbone:pCAGGS; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:18:22 | 26 | ||
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dg263_pENTR_ChrimsonCel Resource Report Resource Website 1+ mentions |
RRID:Addgene_66101 | Chrimson | Caenorhabditis elegans | Kanamycin | PMID:26022242 | This construct was produced through de novo gene synthesis. Chrimson amino acid sequence was published in: Klapoetke NC, Murata Y, Kim SS, Pulver SR, Birdsey-Benson A, Cho YK, Morimoto TK, Chuong AS, Carpenter EJ, Tian Z, Wang J, Xie Y, Yan Z, Zhang Y, Chow BY, Surek B, Melkonian M, Jayaraman V, Constantine-Paton M, Wong GK, and Boyden ES. 2014. Independent optical excitation of distinct neural populations. Nat. Meth. 11, 338-346. | Vector Backbone:pUC57-Kan; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin | 2026-08-15 01:18:24 | 1 | |
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pAH123 Resource Report Resource Website 1+ mentions |
RRID:Addgene_66077 | phi80 integrase | Ampicillin | PMID:25849635 | Vector Backbone:pAH123; Vector Types:; Bacterial Resistance:Ampicillin | 2026-08-15 01:18:23 | 2 | |||
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pET11a+CYP119 Resource Report Resource Website 1+ mentions |
RRID:Addgene_66131 | CYP119 | Other | Ampicillin | PMID:24924478 | We cloned the CYP119 gene from the cell suspension of S. solfataricus P1 strain (ATCC 35091). | Backbone Marker:Novagen; Backbone Size:5639; Vector Backbone:pET-11a; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:18:25 | 1 |
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