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Species: Synthetic
Genetic Insert: sfGFP-iCre-ERT2
Vector Backbone Description: Backbone Marker:Hyung-song Nam, Capecchi laboratory; Vector Backbone:Custom; Vector Types:Mammalian Expression, Cre/Lox, piggyBac; Bacterial Resistance:Ampicillin
Comments: Linker between sfGFP and iCre-ERT2 is a mutated P2A sequence where 2 conserved amino acids are changed to Alanines. This mutated sequence does not skip.
The sfGFP fluorescence from the sfGFP-iCre-ERT2 is dim, as described in Nam and Capecchi, 2020 (https://doi.org/10.1186/s13064-020-00139-5) and a Figshare page (https://doi.org/10.6084/m9.figshare.13218437.v35). In short, it is not visible to the eyes, but can be detected with flow cytometry.
Proper citation: RRID:Addgene_137856 Copy
Species: Synthetic
Genetic Insert: mTagBFP2-T2A-iCreERT2
Vector Backbone Description: Vector Backbone:pCAGGS; Vector Types:Mammalian Expression, Cre/Lox; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_137859 Copy
Species: Synthetic
Genetic Insert: mTagBFP2-T2A-iCreERT2
Vector Backbone Description: Vector Backbone:pCAGGS; Vector Types:Mammalian Expression, Cre/Lox; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_137861 Copy
Species: Synthetic
Genetic Insert: mTagBFP2-T2A-iCreERT2
Vector Backbone Description: Vector Backbone:pCAGGS; Vector Types:Mammalian Expression, Cre/Lox; Bacterial Resistance:Ampicillin
Comments: The depositor recommends the updated versions of this plasmid, PB CAG-sfGFP-T2A-iCreERT2 (https://www.addgene.org/177309/) or PB CAG-mTagBFP2-T2A-iCreERT2 (https://www.addgene.org/177308/).
Proper citation: RRID:Addgene_137864 Copy
Species: Synthetic
Genetic Insert: mTagBFP2-T2A-iCreERT2
Vector Backbone Description: Vector Backbone:pCAGGS; Vector Types:Mammalian Expression, Cre/Lox; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_137863 Copy
Species: Synthetic
Genetic Insert: TRE3G-dCas9-VPR-T2A-EGFP-CAG-TetON-NeoR-SA
Vector Backbone Description: Vector Backbone:modified pAAVS1 (Addgene); Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31959800
Proper citation: RRID:Addgene_137880 Copy
Species: Synthetic
Genetic Insert: mRFP-T2A-BlasticidinR
Vector Backbone Description: Vector Backbone:modified PB-CA (Addgene 20960) ; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:31959800
Proper citation: RRID:Addgene_137881 Copy
Species: Synthetic
Genetic Insert: iAChSnFR (non-binding variant)
Vector Backbone Description: Backbone Size:4373; Vector Backbone:pAAV.hSynap; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Comments: Please visit https://www.biorxiv.org/content/10.1101/2020.02.07.939504v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_137951 Copy
Species: Synthetic
Genetic Insert: iAChSnFR
Vector Backbone Description: Backbone Size:4616; Vector Backbone:pAAV.CAG; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Comments: Please visit https://www.biorxiv.org/content/10.1101/2020.02.07.939504v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_137955 Copy
Species: Synthetic
Genetic Insert: iAChSnFR (yellow variant)
Vector Backbone Description: Backbone Size:5058; Vector Backbone:pAAV.CAGFLEX; Vector Types:Mammalian Expression, AAV, Cre/Lox; Bacterial Resistance:Ampicillin
Comments: Please visit https://www.biorxiv.org/content/10.1101/2020.02.07.939504v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_137959 Copy
Species: Synthetic
Genetic Insert: pSEVA-331Bb-1AG-GFP
Vector Backbone Description: Vector Backbone:pSEVA-331Bb; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:30865830
Comments: Variable expression (depending on strain)
Proper citation: RRID:Addgene_138017 Copy
Species: Synthetic
Genetic Insert: pSEVA-331Bb-2AG-GFP
Vector Backbone Description: Vector Backbone:pSEVA-331Bb; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:30865830
Comments: High expression
Proper citation: RRID:Addgene_138020 Copy
Species: Synthetic
Genetic Insert: Superfolder GFP
Vector Backbone Description: Backbone Marker:Native to E. coli Nissle; Backbone Size:3173; Vector Backbone:pMUT1; Vector Types:Bacterial Expression, Synthetic Biology, For use with E. coli Nissle in the mouse gut; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33301298
Proper citation: RRID:Addgene_137921 Copy
Species: Synthetic
Genetic Insert: mRFP1
Vector Backbone Description: Backbone Marker:Native to E. coli Nissle; Backbone Size:3173; Vector Backbone:pMUT2; Vector Types:Bacterial Expression, Synthetic Biology, For use with E. coli Nissle in the mouse gut; Bacterial Resistance:Tetracycline
Defining Citation: PMID:33301298
Proper citation: RRID:Addgene_137923 Copy
Species: Synthetic
Genetic Insert: J23101-3TG- pSEVA-331Bb- RFP
Vector Backbone Description: Vector Backbone:pSEVA-331Bb; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:30865830
Comments: High expression
Proper citation: RRID:Addgene_138065 Copy
Species: Synthetic
Genetic Insert: J23104-mCherry-10818-GFP-pSEVA-331Bb
Vector Backbone Description: Vector Backbone:pSEVA-331Bb; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:30865830
Comments: Variable expression
Proper citation: RRID:Addgene_138069 Copy
Species: Synthetic
Genetic Insert: J23104-mCherry-10799-GFP-pSEVA-331Bb
Vector Backbone Description: Vector Backbone:pSEVA-331Bb; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:30865830
Comments: High expression
Proper citation: RRID:Addgene_138070 Copy
Species: Synthetic
Genetic Insert: J23104-mCherry-51401-GFP-pSEVA-331Bb
Vector Backbone Description: Vector Backbone:pSEVA-331Bb; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:30865830
Comments: Low expression
Proper citation: RRID:Addgene_138071 Copy
Species: Synthetic
Genetic Insert: J23104-mCherry-L3S3P21-GFP-pSEVA-331Bb
Vector Backbone Description: Vector Backbone:pSEVA-331Bb; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:30865830
Comments: High-intermediate expression
Proper citation: RRID:Addgene_138074 Copy
Species: Synthetic
Genetic Insert: J23104-mCherry-L3S2P44-GFP-pSEVA-331Bb
Vector Backbone Description: Vector Backbone:pSEVA-331Bb; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:30865830
Comments: Low expression
Proper citation: RRID:Addgene_138076 Copy
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