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Species: Synthetic
Genetic Insert: Multiple Cloning Site, NPTII
Vector Backbone Description: Vector Backbone:pCBL101; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:37070560
Proper citation: RRID:Addgene_199724 Copy
Species: Synthetic
Genetic Insert: NPTII
Vector Backbone Description: Vector Backbone:pTF101.1; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:37070560
Proper citation: RRID:Addgene_199722 Copy
Species: Synthetic
Genetic Insert: 35S-RUBY
Vector Backbone Description: Vector Backbone:pCBL101; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:37070560
Comments: Addgene sequencing identified partial duplication of the CaMV 35S enhancer element. Depositing lab has confirmed that the duplication was present in their original stock of the plasmid and does not disrupt RUBY function.
Proper citation: RRID:Addgene_199723 Copy
Species: Synthetic
Genetic Insert: hTfR-GFP
Vector Backbone Description: Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37098770
Comments: Please visit https://doi.org/10.1101/2023.03.03.531050 for bioRxiv preprint. In the original study XL1 Blue E. coli are used for amplifying plasmids.
Proper citation: RRID:Addgene_199714 Copy
Species: Synthetic
Genetic Insert: DARPin-hFc 27F3
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:6800; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36314779
Comments: HTC DARPin name 008-855-2309-D7
Proper citation: RRID:Addgene_199619 Copy
Species: Synthetic
Genetic Insert: DARPin-FLAG 27G2
Vector Backbone Description: Backbone Marker:Qiagen; Backbone Size:4037; Vector Backbone:pQE30; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36314779
Comments: HTC DARPin name 008-855-2308-A9
Proper citation: RRID:Addgene_199616 Copy
Species: Synthetic
Genetic Insert: DARPin-hFc E3_5 (control)
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:6800; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36314779
Comments: HTC DARPin name 008-782-2400-H3
Proper citation: RRID:Addgene_199617 Copy
Species: Synthetic
Genetic Insert: DARPin-FLAG E3_5 (control)
Vector Backbone Description: Backbone Marker:Qiagen; Backbone Size:4037; Vector Backbone:pQE30; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36314779
Comments: HTC DARPin name 008-782-2400-H3
Proper citation: RRID:Addgene_199612 Copy
Species: Synthetic
Genetic Insert: PEmax-P2A-BSD
Vector Backbone Description: Backbone Marker:Hyongbum Kim; Backbone Size:8228; Vector Backbone:pLenti-PE2-BSD; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37119812
Proper citation: RRID:Addgene_191102 Copy
Species: Synthetic
Genetic Insert: NRCH-PE2-BSD
Vector Backbone Description: Backbone Marker:Hyongbum Kim; Backbone Size:8228; Vector Backbone:pLenti-PE2-BSD; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37119812
Proper citation: RRID:Addgene_191101 Copy
Species: Synthetic
Genetic Insert: TriTag
Vector Backbone Description: Vector Backbone:pHR; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36443367
Proper citation: RRID:Addgene_199458 Copy
Species: Synthetic
Genetic Insert: Full length TSWV M antigenome encoding SpCas9 in place of viral GP
Vector Backbone Description: Vector Backbone:pCB301-2μ-HDV; Vector Types:Plant Expression, CRISPR; Bacterial Resistance:Kanamycin
Defining Citation: PMID:36751129
Comments: Note: Plasmid contains a R822Q mutation in the 3xFlag-SV40NLS-Cas9 insert. This mutation is not known to affect plasmid function.
Proper citation: RRID:Addgene_196325 Copy
Species: Synthetic
Genetic Insert: pSP6_Kozak
Vector Backbone Description: Backbone Marker:John Dueber Lab, https://doi.org/10.1021/sb500366v; Vector Backbone:pYTK001; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:37009624
Proper citation: RRID:Addgene_200151 Copy
Species: Synthetic
Genetic Insert: tT7
Vector Backbone Description: Backbone Marker:John Dueber Lab, https://doi.org/10.1021/sb500366v; Vector Backbone:pYTK001; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:37009624
Proper citation: RRID:Addgene_200153 Copy
Species: Synthetic
Genetic Insert: mCherry
Vector Backbone Description: Backbone Marker:Takara; Vector Backbone:pLVSIN-CMV_Pur; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:40399693
Comments: https://benchling.com/s/seq-axx2imqJK8S5TKwg957v?m=slm-g8zVa6H19po3x7oJHpso
Please visit https://www.biorxiv.org/content/10.1101/2023.01.18.524633v1 for bioRxiv preprint. This plasmid is relatively unstable due to repetitive sequences and other genetic element cloned from retrovirus. Please be sure to use NEB stable cells and incubate at 30C.
Proper citation: RRID:Addgene_197137 Copy
Species: Synthetic
Genetic Insert: Full length TSWV M antigenome encoding LbCas12a in place of viral GP
Vector Backbone Description: Vector Backbone:pCB301-2μ-HDV; Vector Types:Plant Expression, CRISPR; Bacterial Resistance:Kanamycin
Defining Citation: PMID:36751129
Comments: Please note: Plasmid contains K191N, E695D, and M985I mutations in LbCas12a. These mutations are not known to affect plasmid function.
Proper citation: RRID:Addgene_196291 Copy
Species: Synthetic
Genetic Insert: HDR donor template
Vector Backbone Description: Vector Backbone:pUC19; Vector Types:CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36443367
Proper citation: RRID:Addgene_199451 Copy
Species: Synthetic
Genetic Insert: TS2 sgRNA
Vector Backbone Description: Vector Backbone:pUC19; Vector Types:CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36443367
Proper citation: RRID:Addgene_199454 Copy
Species: Synthetic
Genetic Insert: HDR donor template
Vector Backbone Description: Vector Backbone:pUC19; Vector Types:CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36443367
Proper citation: RRID:Addgene_199453 Copy
Species: Synthetic
Genetic Insert: stdMCP-PH
Vector Backbone Description: Vector Backbone:pCS2(+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36443367
Proper citation: RRID:Addgene_199461 Copy
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