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Species: Homo sapiens
Genetic Insert: SDCBP
Vector Backbone Description: Vector Backbone:pNIC28-Bsa4; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: Ligation-independent cloning 5' site GTACTTCCAATCCAT; 3' site TATCCACCTTTACT. Gileadi lab uses BL21(DE3)-R3-pRARE2 cells for expression.
Proper citation: RRID:Addgene_175484 Copy
Vector Backbone Description: Backbone Size:5019; Vector Backbone:pDN513; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34599813
Comments: This plasmid was generated by modification of plasmid pRS403 (Sikorski & Hieter, 1989, Genetics 122:19-27) by insertion of CEN6/ARSH4 low copy S. cerevisiae replication and partitioning locus with flanking restriction enzyme cut sites (AatII, AvrII, and SphI).
bioRxiv pre-print 367219: Rapid conversion of replicating and integrating Saccharomyces cerevisiae plasmid vectors via Cre recombinase
https://doi.org/10.1101/2020.11.03.367219
Proper citation: RRID:Addgene_175365 Copy
Vector Backbone Description: Backbone Size:4838; Vector Backbone:pDN514; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34599813
Comments: This plasmid was generated by modification of plasmid pRS404 (Sikorski & Hieter, 1989, Genetics 122:19-27) by insertion of CEN6/ARSH4 low copy S. cerevisiae replication and partitioning locus with flanking restriction enzyme cut sites (AatII, AvrII, and SphI).
bioRxiv pre-print 367219: Rapid conversion of replicating and integrating Saccharomyces cerevisiae plasmid vectors via Cre recombinase
https://doi.org/10.1101/2020.11.03.367219
Proper citation: RRID:Addgene_175366 Copy
Vector Backbone Description: Backbone Size:5825; Vector Backbone:pDN523; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34599813
Comments: This plasmid was generated by modification of plasmid pRS403 (Sikorski & Hieter, 1989, Genetics 122:19-27) by insertion of 2 micron high copy S. cerevisiae replication and partitioning locus with flanking restriction enzyme cut sites (AatII, AvrII, and SphI).
bioRxiv pre-print 367219: Rapid conversion of replicating and integrating Saccharomyces cerevisiae plasmid vectors via Cre recombinase
https://doi.org/10.1101/2020.11.03.367219
Proper citation: RRID:Addgene_175369 Copy
Species: Homo sapiens
Genetic Insert: SDCBP
Vector Backbone Description: Vector Backbone:pNIC28-Bsa4; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: Ligation-independent cloning 5' site GTACTTCCAATCCAT; 3' site TATCCACCTTTACT. Gileadi lab uses BL21(DE3)-R3-pRARE2 cells for expression.
Proper citation: RRID:Addgene_175480 Copy
Vector Backbone Description: Backbone Marker:ExpreS2ion Biotechnologies; Backbone Size:2549; Vector Backbone:pExpreS2-1; Vector Types:Insect Expression; Bacterial Resistance:Bleocin (Zeocin)
Defining Citation: PMID:35600892
Comments: This plasmid is designed for use with the ExpreS2 Platform from ExpreS2ion Biotechnologies. It is intended to be used with the ExpreS2ion cells and media which can be obtained directly from the ExpreS2ion website.
Proper citation: RRID:Addgene_175360 Copy
Species: Homo sapiens
Genetic Insert: SDCBP
Vector Backbone Description: Vector Backbone:pNIC28-Bsa4; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: Ligation-independent cloning 5' site GTACTTCCAATCCAT; 3' site TATCCACCTTTACT. Gileadi lab uses BL21(DE3)-R3-pRARE2 cells for expression.
Proper citation: RRID:Addgene_175482 Copy
Species: Homo sapiens
Genetic Insert: NF2
Vector Backbone Description: Vector Backbone:pNIC28-Bsa4; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: Ligation-independent cloning 5' site GTACTTCCAATCCAT; 3' site TATCCACCTTTACT. Gileadi lab uses BL21(DE3)-R3-pRARE2 cells for expression.
Proper citation: RRID:Addgene_175472 Copy
Species: Homo sapiens
Genetic Insert: PRDX1
Vector Backbone Description: Vector Backbone:pNIC-Bio3; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: Ligation-independent cloning 5' site GTACTTCCAATCCAT; 3' site TATCCACCTTTACT. Gileadi lab uses BL21(DE3)-R3-pRARE2-pBirA cells for expression.
Please note: Plasmid is missing the GSGGGGSGGGGS linker sequence compared to the depositor's sequence. The missing extra linker between the full length coding sequence and Avitag does not affect function of the proteins.
Proper citation: RRID:Addgene_175473 Copy
Species: Homo sapiens
Genetic Insert: RAB5A
Vector Backbone Description: Vector Backbone:pNIC28-Bsa4; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: Ligation-independent cloning 5' site GTACTTCCAATCCAT; 3' site TATCCACCTTTACT. Gileadi lab uses BL21(DE3)-R3-pRARE2 cells for expression.
Proper citation: RRID:Addgene_175475 Copy
Species: Homo sapiens
Genetic Insert: RAB5A
Vector Backbone Description: Vector Backbone:pNIC28-Bsa4; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: Ligation-independent cloning 5' site GTACTTCCAATCCAT; 3' site TATCCACCTTTACT. Gileadi lab uses BL21(DE3)-R3-pRARE2 cells for expression.
Proper citation: RRID:Addgene_175476 Copy
Species: Mus musculus
Genetic Insert: anti-FLAG M2 light chain
Vector Backbone Description: Vector Backbone:pRK5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34121409
Proper citation: RRID:Addgene_175358 Copy
Species: Homo sapiens
Genetic Insert: MSN
Vector Backbone Description: Vector Backbone:pNIC28-Bsa4; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:37866628
Comments: Ligation-independent cloning 5' site GTACTTCCAATCCAT; 3' site TATCCACCTTTACT. Gileadi lab uses BL21(DE3)-R3-pRARE2 cells for expression.
Proper citation: RRID:Addgene_175470 Copy
Species: CoV2
Genetic Insert: nsp1 E55A E57A K58A
Vector Backbone Description: Backbone Size:6028; Vector Backbone:pCDNA4; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34624207
Proper citation: RRID:Addgene_175427 Copy
Species: CoV2
Genetic Insert: nsp1 R119A K120A
Vector Backbone Description: Backbone Size:6028; Vector Backbone:pCDNA4; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34624207
Proper citation: RRID:Addgene_175428 Copy
Species: CoV2
Genetic Insert: nsp1 E36A E37A
Vector Backbone Description: Backbone Size:6028; Vector Backbone:pCDNA4; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34624207
Proper citation: RRID:Addgene_175429 Copy
Species: CoV2
Genetic Insert: CoV2 R99A nsp1
Vector Backbone Description: Backbone Size:6028; Vector Backbone:pCDNA4; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34624207
Proper citation: RRID:Addgene_175422 Copy
Species: Synthetic
Genetic Insert: GFP-Cre recombinase
Vector Backbone Description: Backbone Size:4561; Vector Backbone:pAAV hSyn; Vector Types:AAV; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_175381 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: eRF3
Vector Backbone Description: Backbone Marker:UC Berkeley MacroLab; Vector Backbone:1C; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34413231
Proper citation: RRID:Addgene_175418 Copy
Vector Backbone Description: Backbone Size:5068; Vector Backbone:pDN613; Vector Types:Yeast Expression, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34599813
Comments: This plasmid was generated by modification of plasmid pRS403 (Sikorski & Hieter, 1989, Genetics 122:19-27) by insertion of CEN6/ARSH4 low copy S. cerevisiae replication and partitioning locus with flanking restriction enzyme cut sites (AatII) and flanking LoxP sites.
bioRxiv pre-print 367219: Rapid conversion of replicating and integrating Saccharomyces cerevisiae plasmid vectors via Cre recombinase
https://doi.org/10.1101/2020.11.03.367219
Proper citation: RRID:Addgene_175373 Copy
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