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Plasmid Name Proper Citation Insert Name Organism Bacterial Resistance Defining Citation Comments Vector Backbone Description Relevant Mutation Record Last Update Mentions Count
FoxO1 KA
 
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RRID:Addgene_66164 FoxO1 KA Mus musculus Ampicillin PMID:17681146 Vector Backbone:pBabe bleo; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin K242A, K245A and K262A 2026-08-15 01:18:24 1
pYLCRISPR/Cas9Pubi-B
 
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RRID:Addgene_66188 Cas9 S. pyogenes Kanamycin PMID:25917172 Accession # KR029110. A fragment containing a modified ccdB flanked by two BsaI sites was cloned into the vector to produce the CRISPR/Cas9 binary vector. This can be used for inserting sgRNA cassette. Please see our published protocol for using these plasmids. Ma, X. and Liu, Y.-G. 2016. CRISPR/Cas9-based multiplex genome editing in monocot and dicot plants. Curr. Protoc. Mol. Biol. 115:31.6.1-31.6.21. http://onlinelibrary.wiley.com/doi/10.1002/cpmb.10/abstract doi: 10.1002/cpmb.10 Backbone Marker:CAMBIA ; Vector Backbone:pCAMBIA1300 ; Vector Types:Plant Expression; Bacterial Resistance:Kanamycin plant-codon optimized with higher GC contents (62.5%) in the 5’ region (400 bp) and 54.2% overall GC content 2026-08-15 01:18:24 2
pYLsgRNA-OsU6c
 
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RRID:Addgene_66197 Ampicillin PMID:25917172 Accession number KR029108 Please see our published protocol for using these plasmids. Ma, X. and Liu, Y.-G. 2016. CRISPR/Cas9-based multiplex genome editing in monocot and dicot plants. Curr. Protoc. Mol. Biol. 115:31.6.1-31.6.21. http://onlinelibrary.wiley.com/doi/10.1002/cpmb.10/abstract doi: 10.1002/cpmb.10 Vector Backbone:based on pUC18; Vector Types:Cloning Vector; Bacterial Resistance:Ampicillin 2026-08-15 01:18:24 2
pYLsgRNA-OsU6a/LacZ
 
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RRID:Addgene_66195 Ampicillin PMID:25917172 Accession number KR029106. By Omega-PCR cloning, an E. coli promoter-LacZ sequence (198 bp in length) was inserted. The LacZ marker gene can be used to facilitate selection of Escherichia coli positive CRISPR/Cas9 clones carrying the sgRNA expression cassettes. Please see our published protocol for using these plasmids. Ma, X. and Liu, Y.-G. 2016. CRISPR/Cas9-based multiplex genome editing in monocot and dicot plants. Curr. Protoc. Mol. Biol. 115:31.6.1-31.6.21. http://onlinelibrary.wiley.com/doi/10.1002/cpmb.10/abstract doi: 10.1002/cpmb.10 Vector Backbone:based on pUC18; Vector Types:Cloning Vector; Bacterial Resistance:Ampicillin 2026-08-15 01:18:26 1
pYLsgRNA-OsU3m
 
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RRID:Addgene_66193 Ampicillin PMID:25917172 Accession number KR559259 Please see our published protocol for using these plasmids. Ma, X. and Liu, Y.-G. 2016. CRISPR/Cas9-based multiplex genome editing in monocot and dicot plants. Curr. Protoc. Mol. Biol. 115:31.6.1-31.6.21. http://onlinelibrary.wiley.com/doi/10.1002/cpmb.10/abstract doi: 10.1002/cpmb.10 Vector Backbone:based on pUC18; Vector Types:Cloning Vector; Bacterial Resistance:Ampicillin 2026-08-15 01:18:24 2
pYLCRISPR/Cas9P35S-B
 
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RRID:Addgene_66190 Cas9 S. pyogenes Kanamycin PMID:25917172 Accession # KR029113 A fragment containing a modified ccdB flanked by two BsaI sites was cloned into the vectors to produce the CRISPR/Cas9 binary vectors. This can be used for inserting sgRNA cassette. Please see our published protocol for using these plasmids. Ma, X. and Liu, Y.-G. 2016. CRISPR/Cas9-based multiplex genome editing in monocot and dicot plants. Curr. Protoc. Mol. Biol. 115:31.6.1-31.6.21. http://onlinelibrary.wiley.com/doi/10.1002/cpmb.10/abstract doi: 10.1002/cpmb.10 Backbone Marker:CAMBIA ; Vector Backbone:pCAMBIA1300 ; Vector Types:Plant Expression; Bacterial Resistance:Kanamycin plant-codon optimized with higher GC contents (62.5%) in the 5’ region (400 bp) and 54.2% overall GC content 2026-08-15 01:18:24 4
pYLCRISPR/Cas9P35S-N
 
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RRID:Addgene_66191 Cas9 S. pyogenes Kanamycin PMID:25917172 Accession # KR029112 A fragment containing a modified ccdB flanked by two BsaI sites was cloned into the vectors to produce the CRISPR/Cas9 binary vectors. This can be used for inserting sgRNA cassette. Please see our published protocol for using these plasmids. Ma, X. and Liu, Y.-G. 2016. CRISPR/Cas9-based multiplex genome editing in monocot and dicot plants. Curr. Protoc. Mol. Biol. 115:31.6.1-31.6.21. http://onlinelibrary.wiley.com/doi/10.1002/cpmb.10/abstract doi: 10.1002/cpmb.10 Backbone Marker:CAMBIA ; Vector Backbone:pCAMBIA1300 ; Vector Types:Plant Expression; Bacterial Resistance:Kanamycin plant-codon optimized with higher GC contents (62.5%) in the 5’ region (400 bp) and 54.2% overall GC content 2026-08-15 01:18:24 2
pYLsgRNA-AtU3b/LacZ
 
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RRID:Addgene_66199 Ampicillin PMID:25917172 accession number KR029098. By Omega-PCR cloning, an E. coli promoter-LacZ sequence (198 bp in length) was inserted. The LacZ marker gene can be used to facilitate selection of Escherichia coli positive CRISPR/Cas9 clones carrying the sgRNA expression cassettes. Please see our published protocol for using these plasmids. Ma, X. and Liu, Y.-G. 2016. CRISPR/Cas9-based multiplex genome editing in monocot and dicot plants. Curr. Protoc. Mol. Biol. 115:31.6.1-31.6.21. http://onlinelibrary.wiley.com/doi/10.1002/cpmb.10/abstract doi: 10.1002/cpmb.10 Vector Backbone:based on pUC18; Vector Types:Cloning Vector; Bacterial Resistance:Ampicillin 2026-08-15 01:18:24 2
pYLsgRNA-AtU6-1
 
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1+ mentions
RRID:Addgene_66202 Ampicillin PMID:25917172 Accession number KR029101 Please see our published protocol for using these plasmids. Ma, X. and Liu, Y.-G. 2016. CRISPR/Cas9-based multiplex genome editing in monocot and dicot plants. Curr. Protoc. Mol. Biol. 115:31.6.1-31.6.21. http://onlinelibrary.wiley.com/doi/10.1002/cpmb.10/abstract doi: 10.1002/cpmb.10 Vector Backbone:based on pUC18; Vector Types:Cloning Vector; Bacterial Resistance:Ampicillin 2026-08-15 01:18:25 7
pYLsgRNA-AtU6-29
 
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1+ mentions
RRID:Addgene_66203 Ampicillin PMID:25917172 Accession number KR029102 Please see our published protocol for using these plasmids. Ma, X. and Liu, Y.-G. 2016. CRISPR/Cas9-based multiplex genome editing in monocot and dicot plants. Curr. Protoc. Mol. Biol. 115:31.6.1-31.6.21. http://onlinelibrary.wiley.com/doi/10.1002/cpmb.10/abstract doi: 10.1002/cpmb.10 Vector Backbone:based on pUC18; Vector Types:Cloning Vector; Bacterial Resistance:Ampicillin 2026-08-15 01:18:25 8
pYLsgRNA-AtU3d/LacZ
 
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1+ mentions
RRID:Addgene_66201 Ampicillin PMID:25917172 Accession number KR029100 By Omega-PCR cloning, an E. coli promoter-LacZ sequence (198 bp in length) was inserted. The LacZ marker gene can be used to facilitate selection of Escherichia coli positive CRISPR/Cas9 clones carrying the sgRNA expression cassettes. Please see our published protocol for using these plasmids. Ma, X. and Liu, Y.-G. 2016. CRISPR/Cas9-based multiplex genome editing in monocot and dicot plants. Curr. Protoc. Mol. Biol. 115:31.6.1-31.6.21. http://onlinelibrary.wiley.com/doi/10.1002/cpmb.10/abstract doi: 10.1002/cpmb.10 Vector Backbone:based on pUC18; Vector Types:Cloning Vector; Bacterial Resistance:Ampicillin 2026-08-15 01:18:26 4
pLNHA-C1-HsXRN1
 
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1+ mentions
RRID:Addgene_66596 XRN1 Homo sapiens Kanamycin PMID:23142987 Backbone Marker:Clontech; Backbone Size:4174; Vector Backbone:pLNHA-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin 2026-08-15 01:18:31 2
pCIneo-lambdaN-HA-HsEDC4
 
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1+ mentions
RRID:Addgene_66597 EDC4 Homo sapiens Ampicillin PMID:19966221 Backbone Marker:Promega; Backbone Size:5554; Vector Backbone:pCIneo; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:18:29 1
pETM6-E12-vioABECD
 
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RRID:Addgene_66537 VioA Pseudoalteromonas luteoviolacea Ampicillin PMID:26062452 Backbone Size:5155; Vector Backbone:pETM6; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin 2026-08-15 01:18:28 1
pETM6-mCherry
 
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RRID:Addgene_66534 mCherry Synthetic Ampicillin PMID:26062452 Backbone Size:5155; Vector Backbone:pETM6; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin Codon Optimized for E. coli 2026-08-15 01:18:28 3
pETM6-G6-mCherry
 
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1+ mentions
RRID:Addgene_66533 mCherry Synthetic Ampicillin PMID:26062452 Backbone Size:5155; Vector Backbone:pETM6; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin Codon Optimized for E. coli 2026-08-15 01:18:31 1
pLVXPuro-epsilon-COP-GFP
 
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RRID:Addgene_66604 epsilon COP Bos taurus Ampicillin PMID:20175751 Epsilon COP GFP originally described in Presley, J. F., Ward, T. H., Pfeifer, A. C., Siggia, E. D., Phair, R. D. and Lippincott-Schwartz, J. (2002) Dissection of COPI and Arf1 dynamics in vivo and role in Golgi membrane transport. Nature 417, 187-193 Backbone Marker:Clontech; Vector Backbone:pLVX-Puro; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin 2026-08-15 01:18:29 1
pC13N-iCAG.copGFP
 
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RRID:Addgene_66578 copGFP Synthetic Kanamycin PMID:25587899 Backbone Size:10214; Vector Backbone:pC13N-iRMCE; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin 2026-08-15 01:18:31 9
pSLIK-IDH2-R172K-FLAG
 
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RRID:Addgene_66807 IDH2 Homo sapiens Ampicillin PMID:24882210 Insert gene obtained from Patrick Ward and Craig Thompson in the form of pLPCX-IDH2-R172K The depositor states that this plasmid behaves correctly based on on 2HG production (i.e. the metabolic activity of the enzyme). Backbone Marker:Ian Frasier, Addgene Plasmid #25737; Backbone Size:13918; Vector Backbone:pSLIK-hygro; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin R172K 2026-08-15 01:18:29 3
pSLIK-IDH1-FLAG
 
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RRID:Addgene_66802 IDH1 Homo sapiens Ampicillin PMID:24882210 Backbone Marker:Ian Frasier, Addgene Plasmid #25737; Backbone Size:13918; Vector Backbone:pSLIK-hygro; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin 2026-08-15 01:18:29 6

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