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| Plasmid Name | Proper Citation | Insert Name | Organism | Bacterial Resistance | Defining Citation |
Comments |
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FoxO1 KA Resource Report Resource Website 1+ mentions |
RRID:Addgene_66164 | FoxO1 KA | Mus musculus | Ampicillin | PMID:17681146 | Vector Backbone:pBabe bleo; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin | K242A, K245A and K262A | 2026-08-15 01:18:24 | 1 | |
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pYLCRISPR/Cas9Pubi-B Resource Report Resource Website 1+ mentions |
RRID:Addgene_66188 | Cas9 | S. pyogenes | Kanamycin | PMID:25917172 | Accession # KR029110. A fragment containing a modified ccdB flanked by two BsaI sites was cloned into the vector to produce the CRISPR/Cas9 binary vector. This can be used for inserting sgRNA cassette. Please see our published protocol for using these plasmids. Ma, X. and Liu, Y.-G. 2016. CRISPR/Cas9-based multiplex genome editing in monocot and dicot plants. Curr. Protoc. Mol. Biol. 115:31.6.1-31.6.21. http://onlinelibrary.wiley.com/doi/10.1002/cpmb.10/abstract doi: 10.1002/cpmb.10 | Backbone Marker:CAMBIA ; Vector Backbone:pCAMBIA1300 ; Vector Types:Plant Expression; Bacterial Resistance:Kanamycin | plant-codon optimized with higher GC contents (62.5%) in the 5’ region (400 bp) and 54.2% overall GC content | 2026-08-15 01:18:24 | 2 |
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pYLsgRNA-OsU6c Resource Report Resource Website 1+ mentions |
RRID:Addgene_66197 | Ampicillin | PMID:25917172 | Accession number KR029108 Please see our published protocol for using these plasmids. Ma, X. and Liu, Y.-G. 2016. CRISPR/Cas9-based multiplex genome editing in monocot and dicot plants. Curr. Protoc. Mol. Biol. 115:31.6.1-31.6.21. http://onlinelibrary.wiley.com/doi/10.1002/cpmb.10/abstract doi: 10.1002/cpmb.10 | Vector Backbone:based on pUC18; Vector Types:Cloning Vector; Bacterial Resistance:Ampicillin | 2026-08-15 01:18:24 | 2 | |||
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pYLsgRNA-OsU6a/LacZ Resource Report Resource Website 1+ mentions |
RRID:Addgene_66195 | Ampicillin | PMID:25917172 | Accession number KR029106. By Omega-PCR cloning, an E. coli promoter-LacZ sequence (198 bp in length) was inserted. The LacZ marker gene can be used to facilitate selection of Escherichia coli positive CRISPR/Cas9 clones carrying the sgRNA expression cassettes. Please see our published protocol for using these plasmids. Ma, X. and Liu, Y.-G. 2016. CRISPR/Cas9-based multiplex genome editing in monocot and dicot plants. Curr. Protoc. Mol. Biol. 115:31.6.1-31.6.21. http://onlinelibrary.wiley.com/doi/10.1002/cpmb.10/abstract doi: 10.1002/cpmb.10 | Vector Backbone:based on pUC18; Vector Types:Cloning Vector; Bacterial Resistance:Ampicillin | 2026-08-15 01:18:26 | 1 | |||
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pYLsgRNA-OsU3m Resource Report Resource Website 1+ mentions |
RRID:Addgene_66193 | Ampicillin | PMID:25917172 | Accession number KR559259 Please see our published protocol for using these plasmids. Ma, X. and Liu, Y.-G. 2016. CRISPR/Cas9-based multiplex genome editing in monocot and dicot plants. Curr. Protoc. Mol. Biol. 115:31.6.1-31.6.21. http://onlinelibrary.wiley.com/doi/10.1002/cpmb.10/abstract doi: 10.1002/cpmb.10 | Vector Backbone:based on pUC18; Vector Types:Cloning Vector; Bacterial Resistance:Ampicillin | 2026-08-15 01:18:24 | 2 | |||
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pYLCRISPR/Cas9P35S-B Resource Report Resource Website 1+ mentions |
RRID:Addgene_66190 | Cas9 | S. pyogenes | Kanamycin | PMID:25917172 | Accession # KR029113 A fragment containing a modified ccdB flanked by two BsaI sites was cloned into the vectors to produce the CRISPR/Cas9 binary vectors. This can be used for inserting sgRNA cassette. Please see our published protocol for using these plasmids. Ma, X. and Liu, Y.-G. 2016. CRISPR/Cas9-based multiplex genome editing in monocot and dicot plants. Curr. Protoc. Mol. Biol. 115:31.6.1-31.6.21. http://onlinelibrary.wiley.com/doi/10.1002/cpmb.10/abstract doi: 10.1002/cpmb.10 | Backbone Marker:CAMBIA ; Vector Backbone:pCAMBIA1300 ; Vector Types:Plant Expression; Bacterial Resistance:Kanamycin | plant-codon optimized with higher GC contents (62.5%) in the 5’ region (400 bp) and 54.2% overall GC content | 2026-08-15 01:18:24 | 4 |
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pYLCRISPR/Cas9P35S-N Resource Report Resource Website 1+ mentions |
RRID:Addgene_66191 | Cas9 | S. pyogenes | Kanamycin | PMID:25917172 | Accession # KR029112 A fragment containing a modified ccdB flanked by two BsaI sites was cloned into the vectors to produce the CRISPR/Cas9 binary vectors. This can be used for inserting sgRNA cassette. Please see our published protocol for using these plasmids. Ma, X. and Liu, Y.-G. 2016. CRISPR/Cas9-based multiplex genome editing in monocot and dicot plants. Curr. Protoc. Mol. Biol. 115:31.6.1-31.6.21. http://onlinelibrary.wiley.com/doi/10.1002/cpmb.10/abstract doi: 10.1002/cpmb.10 | Backbone Marker:CAMBIA ; Vector Backbone:pCAMBIA1300 ; Vector Types:Plant Expression; Bacterial Resistance:Kanamycin | plant-codon optimized with higher GC contents (62.5%) in the 5’ region (400 bp) and 54.2% overall GC content | 2026-08-15 01:18:24 | 2 |
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pYLsgRNA-AtU3b/LacZ Resource Report Resource Website 1+ mentions |
RRID:Addgene_66199 | Ampicillin | PMID:25917172 | accession number KR029098. By Omega-PCR cloning, an E. coli promoter-LacZ sequence (198 bp in length) was inserted. The LacZ marker gene can be used to facilitate selection of Escherichia coli positive CRISPR/Cas9 clones carrying the sgRNA expression cassettes. Please see our published protocol for using these plasmids. Ma, X. and Liu, Y.-G. 2016. CRISPR/Cas9-based multiplex genome editing in monocot and dicot plants. Curr. Protoc. Mol. Biol. 115:31.6.1-31.6.21. http://onlinelibrary.wiley.com/doi/10.1002/cpmb.10/abstract doi: 10.1002/cpmb.10 | Vector Backbone:based on pUC18; Vector Types:Cloning Vector; Bacterial Resistance:Ampicillin | 2026-08-15 01:18:24 | 2 | |||
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pYLsgRNA-AtU6-1 Resource Report Resource Website 1+ mentions |
RRID:Addgene_66202 | Ampicillin | PMID:25917172 | Accession number KR029101 Please see our published protocol for using these plasmids. Ma, X. and Liu, Y.-G. 2016. CRISPR/Cas9-based multiplex genome editing in monocot and dicot plants. Curr. Protoc. Mol. Biol. 115:31.6.1-31.6.21. http://onlinelibrary.wiley.com/doi/10.1002/cpmb.10/abstract doi: 10.1002/cpmb.10 | Vector Backbone:based on pUC18; Vector Types:Cloning Vector; Bacterial Resistance:Ampicillin | 2026-08-15 01:18:25 | 7 | |||
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pYLsgRNA-AtU6-29 Resource Report Resource Website 1+ mentions |
RRID:Addgene_66203 | Ampicillin | PMID:25917172 | Accession number KR029102 Please see our published protocol for using these plasmids. Ma, X. and Liu, Y.-G. 2016. CRISPR/Cas9-based multiplex genome editing in monocot and dicot plants. Curr. Protoc. Mol. Biol. 115:31.6.1-31.6.21. http://onlinelibrary.wiley.com/doi/10.1002/cpmb.10/abstract doi: 10.1002/cpmb.10 | Vector Backbone:based on pUC18; Vector Types:Cloning Vector; Bacterial Resistance:Ampicillin | 2026-08-15 01:18:25 | 8 | |||
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pYLsgRNA-AtU3d/LacZ Resource Report Resource Website 1+ mentions |
RRID:Addgene_66201 | Ampicillin | PMID:25917172 | Accession number KR029100 By Omega-PCR cloning, an E. coli promoter-LacZ sequence (198 bp in length) was inserted. The LacZ marker gene can be used to facilitate selection of Escherichia coli positive CRISPR/Cas9 clones carrying the sgRNA expression cassettes. Please see our published protocol for using these plasmids. Ma, X. and Liu, Y.-G. 2016. CRISPR/Cas9-based multiplex genome editing in monocot and dicot plants. Curr. Protoc. Mol. Biol. 115:31.6.1-31.6.21. http://onlinelibrary.wiley.com/doi/10.1002/cpmb.10/abstract doi: 10.1002/cpmb.10 | Vector Backbone:based on pUC18; Vector Types:Cloning Vector; Bacterial Resistance:Ampicillin | 2026-08-15 01:18:26 | 4 | |||
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pLNHA-C1-HsXRN1 Resource Report Resource Website 1+ mentions |
RRID:Addgene_66596 | XRN1 | Homo sapiens | Kanamycin | PMID:23142987 | Backbone Marker:Clontech; Backbone Size:4174; Vector Backbone:pLNHA-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin | 2026-08-15 01:18:31 | 2 | ||
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pCIneo-lambdaN-HA-HsEDC4 Resource Report Resource Website 1+ mentions |
RRID:Addgene_66597 | EDC4 | Homo sapiens | Ampicillin | PMID:19966221 | Backbone Marker:Promega; Backbone Size:5554; Vector Backbone:pCIneo; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:18:29 | 1 | ||
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pETM6-E12-vioABECD Resource Report Resource Website 1+ mentions |
RRID:Addgene_66537 | VioA | Pseudoalteromonas luteoviolacea | Ampicillin | PMID:26062452 | Backbone Size:5155; Vector Backbone:pETM6; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin | 2026-08-15 01:18:28 | 1 | ||
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pETM6-mCherry Resource Report Resource Website 1+ mentions |
RRID:Addgene_66534 | mCherry | Synthetic | Ampicillin | PMID:26062452 | Backbone Size:5155; Vector Backbone:pETM6; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin | Codon Optimized for E. coli | 2026-08-15 01:18:28 | 3 | |
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pETM6-G6-mCherry Resource Report Resource Website 1+ mentions |
RRID:Addgene_66533 | mCherry | Synthetic | Ampicillin | PMID:26062452 | Backbone Size:5155; Vector Backbone:pETM6; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin | Codon Optimized for E. coli | 2026-08-15 01:18:31 | 1 | |
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pLVXPuro-epsilon-COP-GFP Resource Report Resource Website 1+ mentions |
RRID:Addgene_66604 | epsilon COP | Bos taurus | Ampicillin | PMID:20175751 | Epsilon COP GFP originally described in Presley, J. F., Ward, T. H., Pfeifer, A. C., Siggia, E. D., Phair, R. D. and Lippincott-Schwartz, J. (2002) Dissection of COPI and Arf1 dynamics in vivo and role in Golgi membrane transport. Nature 417, 187-193 | Backbone Marker:Clontech; Vector Backbone:pLVX-Puro; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin | 2026-08-15 01:18:29 | 1 | |
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pC13N-iCAG.copGFP Resource Report Resource Website 1+ mentions |
RRID:Addgene_66578 | copGFP | Synthetic | Kanamycin | PMID:25587899 | Backbone Size:10214; Vector Backbone:pC13N-iRMCE; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin | 2026-08-15 01:18:31 | 9 | ||
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pSLIK-IDH2-R172K-FLAG Resource Report Resource Website 1+ mentions |
RRID:Addgene_66807 | IDH2 | Homo sapiens | Ampicillin | PMID:24882210 | Insert gene obtained from Patrick Ward and Craig Thompson in the form of pLPCX-IDH2-R172K The depositor states that this plasmid behaves correctly based on on 2HG production (i.e. the metabolic activity of the enzyme). | Backbone Marker:Ian Frasier, Addgene Plasmid #25737; Backbone Size:13918; Vector Backbone:pSLIK-hygro; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin | R172K | 2026-08-15 01:18:29 | 3 |
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pSLIK-IDH1-FLAG Resource Report Resource Website 1+ mentions |
RRID:Addgene_66802 | IDH1 | Homo sapiens | Ampicillin | PMID:24882210 | Backbone Marker:Ian Frasier, Addgene Plasmid #25737; Backbone Size:13918; Vector Backbone:pSLIK-hygro; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin | 2026-08-15 01:18:29 | 6 |
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