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Species: Mus musculus
Genetic Insert: FoxO1 KA
Vector Backbone Description: Vector Backbone:pBabe bleo; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17681146
Proper citation: RRID:Addgene_66164 Copy
Species: S. pyogenes
Genetic Insert: Cas9
Vector Backbone Description: Backbone Marker:CAMBIA ; Vector Backbone:pCAMBIA1300 ; Vector Types:Plant Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25917172
Comments: Accession # KR029110. A fragment containing a modified ccdB flanked by two BsaI sites was cloned into the vector to produce the CRISPR/Cas9 binary vector. This can be used for inserting sgRNA cassette.
Please see our published protocol for using these plasmids. Ma, X. and Liu, Y.-G. 2016. CRISPR/Cas9-based multiplex genome editing in monocot and dicot plants. Curr. Protoc. Mol. Biol. 115:31.6.1-31.6.21. http://onlinelibrary.wiley.com/doi/10.1002/cpmb.10/abstract doi: 10.1002/cpmb.10
Proper citation: RRID:Addgene_66188 Copy
Vector Backbone Description: Vector Backbone:based on pUC18; Vector Types:Cloning Vector; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25917172
Comments: Accession number KR029108
Please see our published protocol for using these plasmids. Ma, X. and Liu, Y.-G. 2016. CRISPR/Cas9-based multiplex genome editing in monocot and dicot plants. Curr. Protoc. Mol. Biol. 115:31.6.1-31.6.21. http://onlinelibrary.wiley.com/doi/10.1002/cpmb.10/abstract doi: 10.1002/cpmb.10
Proper citation: RRID:Addgene_66197 Copy
Vector Backbone Description: Vector Backbone:based on pUC18; Vector Types:Cloning Vector; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25917172
Comments: Accession number KR029106. By Omega-PCR cloning, an E. coli promoter-LacZ sequence (198 bp in length) was inserted. The LacZ marker gene can be used to facilitate selection of Escherichia coli positive CRISPR/Cas9 clones carrying the sgRNA expression cassettes.
Please see our published protocol for using these plasmids. Ma, X. and Liu, Y.-G. 2016. CRISPR/Cas9-based multiplex genome editing in monocot and dicot plants. Curr. Protoc. Mol. Biol. 115:31.6.1-31.6.21. http://onlinelibrary.wiley.com/doi/10.1002/cpmb.10/abstract doi: 10.1002/cpmb.10
Proper citation: RRID:Addgene_66195 Copy
Vector Backbone Description: Vector Backbone:based on pUC18; Vector Types:Cloning Vector; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25917172
Comments: Accession number KR559259
Please see our published protocol for using these plasmids. Ma, X. and Liu, Y.-G. 2016. CRISPR/Cas9-based multiplex genome editing in monocot and dicot plants. Curr. Protoc. Mol. Biol. 115:31.6.1-31.6.21. http://onlinelibrary.wiley.com/doi/10.1002/cpmb.10/abstract doi: 10.1002/cpmb.10
Proper citation: RRID:Addgene_66193 Copy
Species: S. pyogenes
Genetic Insert: Cas9
Vector Backbone Description: Backbone Marker:CAMBIA ; Vector Backbone:pCAMBIA1300 ; Vector Types:Plant Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25917172
Comments: Accession # KR029113 A fragment containing a modified ccdB flanked by two BsaI sites was cloned into the vectors to produce the CRISPR/Cas9 binary vectors. This can be used for inserting sgRNA cassette.
Please see our published protocol for using these plasmids. Ma, X. and Liu, Y.-G. 2016. CRISPR/Cas9-based multiplex genome editing in monocot and dicot plants. Curr. Protoc. Mol. Biol. 115:31.6.1-31.6.21. http://onlinelibrary.wiley.com/doi/10.1002/cpmb.10/abstract doi: 10.1002/cpmb.10
Proper citation: RRID:Addgene_66190 Copy
Species: S. pyogenes
Genetic Insert: Cas9
Vector Backbone Description: Backbone Marker:CAMBIA ; Vector Backbone:pCAMBIA1300 ; Vector Types:Plant Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25917172
Comments: Accession # KR029112 A fragment containing a modified ccdB flanked by two BsaI sites was cloned into the vectors to produce the CRISPR/Cas9 binary vectors. This can be used for inserting sgRNA cassette.
Please see our published protocol for using these plasmids. Ma, X. and Liu, Y.-G. 2016. CRISPR/Cas9-based multiplex genome editing in monocot and dicot plants. Curr. Protoc. Mol. Biol. 115:31.6.1-31.6.21. http://onlinelibrary.wiley.com/doi/10.1002/cpmb.10/abstract doi: 10.1002/cpmb.10
Proper citation: RRID:Addgene_66191 Copy
Vector Backbone Description: Vector Backbone:based on pUC18; Vector Types:Cloning Vector; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25917172
Comments: accession number KR029098. By Omega-PCR cloning, an E. coli promoter-LacZ sequence (198 bp in length) was inserted. The LacZ marker gene can be used to facilitate selection of Escherichia coli positive CRISPR/Cas9 clones carrying the sgRNA expression cassettes.
Please see our published protocol for using these plasmids. Ma, X. and Liu, Y.-G. 2016. CRISPR/Cas9-based multiplex genome editing in monocot and dicot plants. Curr. Protoc. Mol. Biol. 115:31.6.1-31.6.21. http://onlinelibrary.wiley.com/doi/10.1002/cpmb.10/abstract doi: 10.1002/cpmb.10
Proper citation: RRID:Addgene_66199 Copy
Vector Backbone Description: Vector Backbone:based on pUC18; Vector Types:Cloning Vector; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25917172
Comments: Accession number KR029101
Please see our published protocol for using these plasmids. Ma, X. and Liu, Y.-G. 2016. CRISPR/Cas9-based multiplex genome editing in monocot and dicot plants. Curr. Protoc. Mol. Biol. 115:31.6.1-31.6.21. http://onlinelibrary.wiley.com/doi/10.1002/cpmb.10/abstract doi: 10.1002/cpmb.10
Proper citation: RRID:Addgene_66202 Copy
Vector Backbone Description: Vector Backbone:based on pUC18; Vector Types:Cloning Vector; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25917172
Comments: Accession number KR029102
Please see our published protocol for using these plasmids. Ma, X. and Liu, Y.-G. 2016. CRISPR/Cas9-based multiplex genome editing in monocot and dicot plants. Curr. Protoc. Mol. Biol. 115:31.6.1-31.6.21. http://onlinelibrary.wiley.com/doi/10.1002/cpmb.10/abstract doi: 10.1002/cpmb.10
Proper citation: RRID:Addgene_66203 Copy
Vector Backbone Description: Vector Backbone:based on pUC18; Vector Types:Cloning Vector; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25917172
Comments: Accession number KR029100 By Omega-PCR cloning, an E. coli promoter-LacZ sequence (198 bp in length) was inserted. The LacZ marker gene can be used to facilitate selection of Escherichia coli positive CRISPR/Cas9 clones carrying the sgRNA expression cassettes.
Please see our published protocol for using these plasmids. Ma, X. and Liu, Y.-G. 2016. CRISPR/Cas9-based multiplex genome editing in monocot and dicot plants. Curr. Protoc. Mol. Biol. 115:31.6.1-31.6.21. http://onlinelibrary.wiley.com/doi/10.1002/cpmb.10/abstract doi: 10.1002/cpmb.10
Proper citation: RRID:Addgene_66201 Copy
Species: Homo sapiens
Genetic Insert: XRN1
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4174; Vector Backbone:pLNHA-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:23142987
Proper citation: RRID:Addgene_66596 Copy
Species: Homo sapiens
Genetic Insert: EDC4
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:5554; Vector Backbone:pCIneo; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19966221
Proper citation: RRID:Addgene_66597 Copy
Species: Pseudoalteromonas luteoviolacea
Genetic Insert: VioA
Vector Backbone Description: Backbone Size:5155; Vector Backbone:pETM6; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26062452
Proper citation: RRID:Addgene_66537 Copy
Species: Synthetic
Genetic Insert: mCherry
Vector Backbone Description: Backbone Size:5155; Vector Backbone:pETM6; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26062452
Proper citation: RRID:Addgene_66534 Copy
Species: Synthetic
Genetic Insert: mCherry
Vector Backbone Description: Backbone Size:5155; Vector Backbone:pETM6; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26062452
Proper citation: RRID:Addgene_66533 Copy
Species: Bos taurus
Genetic Insert: epsilon COP
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pLVX-Puro; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20175751
Comments: Epsilon COP GFP originally described in
Presley, J. F., Ward, T. H., Pfeifer, A. C., Siggia, E. D., Phair, R. D. and Lippincott-Schwartz, J. (2002) Dissection of COPI and Arf1 dynamics in vivo and role in Golgi membrane transport. Nature 417, 187-193
Proper citation: RRID:Addgene_66604 Copy
Species: Synthetic
Genetic Insert: copGFP
Vector Backbone Description: Backbone Size:10214; Vector Backbone:pC13N-iRMCE; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25587899
Proper citation: RRID:Addgene_66578 Copy
Species: Homo sapiens
Genetic Insert: IDH2
Vector Backbone Description: Backbone Marker:Ian Frasier, Addgene Plasmid #25737; Backbone Size:13918; Vector Backbone:pSLIK-hygro; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24882210
Comments: Insert gene obtained from Patrick Ward and Craig Thompson in the form of pLPCX-IDH2-R172K
The depositor states that this plasmid behaves correctly based on on 2HG production (i.e. the metabolic activity of the enzyme).
Proper citation: RRID:Addgene_66807 Copy
Species: Homo sapiens
Genetic Insert: IDH1
Vector Backbone Description: Backbone Marker:Ian Frasier, Addgene Plasmid #25737; Backbone Size:13918; Vector Backbone:pSLIK-hygro; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24882210
Proper citation: RRID:Addgene_66802 Copy
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