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Species: Other
Genetic Insert: CDS, lv 0, mKate-2
Vector Backbone Description: Backbone Marker:Georg Fritz and Thorsten Mascher; Vector Backbone:Synthetic; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:40650977
Proper citation: RRID:Addgene_249918 Copy
Species: Other
Genetic Insert: C-terminal tag, lv0, mKate-2
Vector Backbone Description: Backbone Marker:Georg Fritz and Thorsten Mascher; Vector Backbone:Synthetic; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:40650977
Proper citation: RRID:Addgene_249947 Copy
Species: Other
Genetic Insert: C-terminal tag, lv0, sfGfp
Vector Backbone Description: Backbone Marker:Georg Fritz and Thorsten Mascher; Vector Backbone:Synthetic; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:40650977
Proper citation: RRID:Addgene_249948 Copy
Species: Other
Genetic Insert: NT-CRISPR plasmid (pST_116) with gRNA targetting phaBAC
Vector Backbone Description: Backbone Marker:Georg Fritz and Thorsten Mascher; Vector Backbone:Synthetic; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:40650977
Proper citation: RRID:Addgene_250052 Copy
Species: Other
Genetic Insert: dropout part, lv0, pos 2, mScarlet-I
Vector Backbone Description: Backbone Marker:Georg Fritz and Thorsten Mascher; Vector Backbone:Synthetic; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:40650977
Proper citation: RRID:Addgene_249992 Copy
Species: Other
Genetic Insert: ABR, lv0, Akan, mScarlet-I
Vector Backbone Description: Backbone Marker:Georg Fritz and Thorsten Mascher; Vector Backbone:Synthetic; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:40650977
Proper citation: RRID:Addgene_249986 Copy
Species: Other
Genetic Insert: dropout part, lv0*, 1R-5O, mScarlet-I
Vector Backbone Description: Backbone Marker:Georg Fritz and Thorsten Mascher; Vector Backbone:Synthetic; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:40650977
Proper citation: RRID:Addgene_250041 Copy
Species: Other
Genetic Insert: Ba1Cas12a3 E. coli codon optimized C-terminal His tag
Vector Backbone Description: Backbone Marker:Twist; Vector Backbone:pET-29b(+); Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:41501459
Proper citation: RRID:Addgene_253635 Copy
Species: Other
Genetic Insert: Sm3Cas12a3 E. coli codon optimized C-terminal His tag
Vector Backbone Description: Backbone Marker:Twist; Vector Backbone:pET-29b(+); Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:41501459
Proper citation: RRID:Addgene_253637 Copy
Species: Other
Genetic Insert: ApCas12a4 E. coli codon optimized C-terminal His tag
Vector Backbone Description: Backbone Marker:Twist; Vector Backbone:pET-29b(+); Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:41501459
Proper citation: RRID:Addgene_253639 Copy
Species: Other
Genetic Insert: ABR, lv0, Atet, sfGFP
Vector Backbone Description: Backbone Marker:Georg Fritz and Thorsten Mascher; Vector Backbone:Synthetic; Vector Types:Synthetic Biology; Bacterial Resistance:Tetracycline
Defining Citation: PMID:40650977
Proper citation: RRID:Addgene_249981 Copy
Species: Other
Genetic Insert: ABR, lv0*, Atet, sfGFP
Vector Backbone Description: Backbone Marker:Georg Fritz and Thorsten Mascher; Vector Backbone:Synthetic; Vector Types:Synthetic Biology; Bacterial Resistance:Tetracycline
Defining Citation: PMID:40650977
Proper citation: RRID:Addgene_250022 Copy
Species: Other
Genetic Insert: MpCas12a E. coli codon optimized C-terminal His tag
Vector Backbone Description: Backbone Marker:Twist; Vector Backbone:pET-29b(+); Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:41501459
Proper citation: RRID:Addgene_253634 Copy
Species: Other
Genetic Insert: J23119 CAO1 target + CAO1 5x array targeting
Vector Backbone Description: Vector Backbone:p15A; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:41501459
Proper citation: RRID:Addgene_253627 Copy
Species: Other
Genetic Insert: J23119 GFP T14 target + GFP T14 5x array targeting
Vector Backbone Description: Vector Backbone:p15A; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:41501459
Proper citation: RRID:Addgene_253631 Copy
Species: Other
Genetic Insert: J23119 GFP T14 target + CAO1 5x array non-targeting
Vector Backbone Description: Vector Backbone:p15A; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:41501459
Proper citation: RRID:Addgene_253632 Copy
Species: Other
Genetic Insert: IS1_TS1-A-PgpdA-uidA-TtrpC-B-IS1_TS2-ori-AmpR
Vector Backbone Description: Vector Backbone:pU2002; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Comments: Please visit https://doi.org/10.1101/2024.09.13.612835 for bioRxiv preprint.
Proper citation: RRID:Addgene_247963 Copy
Species: Other
Genetic Insert: Geo Sec Tag
Vector Backbone Description: Backbone Marker:iGEM; Vector Backbone:pSB1A2; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Proper citation: RRID:Addgene_235442 Copy
Species: Other
Genetic Insert: sfGFP Dropout Cassette
Vector Backbone Description: Vector Backbone:pS797; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: To enable the conjugal transformation of Geobacillus species. plasmid pS797 was engineered to include an origin of transfer (ORI T) that incorporates the Nic region and traJ gene from the RP4 conjugal plasmid. Additionally, it features two replication origins-ColE for E. coli and BST1 for Geobacillus-to support propagation in both hosts. The plasmid also carries two antibiotic resistance markers, which allow for selection with ampicillin in E. coli and kanamycin in Geobacillus.
Proper citation: RRID:Addgene_235444 Copy
Species: Other
Genetic Insert: lacZ Dropout Cassette
Vector Backbone Description: Vector Backbone:pS797; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: To enable the conjugal transformation of Geobacillus species. plasmid pS797 was engineered to include an origin of transfer (ORI T) that incorporates the Nic region and traJ gene from the RP4 conjugal plasmid. Additionally, it features two replication origins-ColE for E. coli and BST1 for Geobacillus-to support propagation in both hosts. The plasmid also carries two antibiotic resistance markers, which allow for selection with ampicillin in E. coli and kanamycin in Geobacillus.
Proper citation: RRID:Addgene_235446 Copy
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