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Vector Backbone Description: Backbone Size:4887; Vector Backbone:pDN614; Vector Types:Yeast Expression, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34599813
Comments: This plasmid was generated by modification of plasmid pRS404 (Sikorski & Hieter, 1989, Genetics 122:19-27) by insertion of CEN6/ARSH4 low copy S. cerevisiae replication and partitioning locus with flanking restriction enzyme cut sites (AatII) and flanking LoxP sites.
bioRxiv pre-print 367219: Rapid conversion of replicating and integrating Saccharomyces cerevisiae plasmid vectors via Cre recombinase
https://doi.org/10.1101/2020.11.03.367219
Proper citation: RRID:Addgene_175374 Copy
Vector Backbone Description: Backbone Size:4985; Vector Backbone:pDN616; Vector Types:Yeast Expression, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34599813
Comments: This plasmid was generated by modification of plasmid pRS406 (Sikorski & Hieter, 1989, Genetics 122:19-27) by insertion of CEN6/ARSH4 low copy S. cerevisiae replication and partitioning locus with flanking restriction enzyme cut sites (AatII) and flanking LoxP sites.
bioRxiv pre-print 367219: Rapid conversion of replicating and integrating Saccharomyces cerevisiae plasmid vectors via Cre recombinase
https://doi.org/10.1101/2020.11.03.367219
Proper citation: RRID:Addgene_175376 Copy
Vector Backbone Description: Backbone Size:6914; Vector Backbone:pDN625; Vector Types:Yeast Expression, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34599813
Comments: This plasmid was generated by modification of plasmid pRS405 (Sikorski & Hieter, 1989, Genetics 122:19-27) by insertion of 2 micron high copy S. cerevisiae replication and partitioning locus with flanking restriction enzyme cut sites (AatII) and flanking LoxP sites.
bioRxiv pre-print 367219: Rapid conversion of replicating and integrating Saccharomyces cerevisiae plasmid vectors via Cre recombinase
https://doi.org/10.1101/2020.11.03.367219
Proper citation: RRID:Addgene_175379 Copy
Vector Backbone Description: Backbone Size:6865; Vector Backbone:pDN525; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34599813
Comments: This plasmid was generated by modification of plasmid pRS405 (Sikorski & Hieter, 1989, Genetics 122:19-27) by insertion of 2 micron high copy S. cerevisiae replication and partitioning locus with flanking restriction enzyme cut sites (AatII, AvrII, and SphI).
bioRxiv pre-print 367219: Rapid conversion of replicating and integrating Saccharomyces cerevisiae plasmid vectors via Cre recombinase
https://doi.org/10.1101/2020.11.03.367219
Proper citation: RRID:Addgene_175371 Copy
Vector Backbone Description: Backbone Marker:ExpreS2ion Biotechnologies; Backbone Size:3156; Vector Backbone:pExpreS2-1; Vector Types:Insect Expression; Bacterial Resistance:Bleocin (Zeocin)
Defining Citation: PMID:35600892
Comments: This plasmid is designed for use with the ExpreS2 Platform from ExpreS2ion Biotechnologies. It is intended to be used with the ExpreS2ion cells and media which can be obtained directly from the ExpreS2ion website.
Proper citation: RRID:Addgene_175447 Copy
Species: Homo sapiens
Genetic Insert: CD44
Vector Backbone Description: Vector Backbone:pNIC28-Bsa4; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: Ligation-independent cloning 5' site GTACTTCCAATCCAT; 3' site TATCCACCTTTACT. Gileadi lab uses BL21(DE3)-R3-pRARE2 cells for expression.
Proper citation: RRID:Addgene_175448 Copy
Species: Homo sapiens
Genetic Insert: CD44
Vector Backbone Description: Vector Backbone:pNIC-CTH0; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: Ligation-independent cloning 5' site GTACTTCCAATCCAT; 3' site TATCCACCTTTACT. Gileadi lab uses BL21(DE3)-R3-pRARE2 cells for expression.
Proper citation: RRID:Addgene_175449 Copy
Species: Synthetic
Genetic Insert: TVA-P2A-eGFP-P2A-N2cG
Vector Backbone Description: Backbone Marker:Jordane Dimidschstein (Addgene plasmid # 170856); Vector Backbone:pAAV-VTKS5; Vector Types:AAV, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34758329
Proper citation: RRID:Addgene_175440 Copy
Vector Backbone Description: Backbone Marker:ExpreS2ion Biotechnologies; Backbone Size:2763; Vector Backbone:pExpreS2-1; Vector Types:Insect Expression; Bacterial Resistance:Bleocin (Zeocin)
Defining Citation: PMID:35600892
Comments: This plasmid is designed for use with the ExpreS2 Platform from ExpreS2ion Biotechnologies. It is intended to be used with the ExpreS2ion cells and media which can be obtained directly from the ExpreS2ion website.
Proper citation: RRID:Addgene_175444 Copy
Vector Backbone Description: Backbone Marker:ExpreS2ion Biotechnologies; Backbone Size:3156; Vector Backbone:pExpreS2-1; Vector Types:Insect Expression; Bacterial Resistance:Bleocin (Zeocin)
Defining Citation: PMID:35600892
Comments: This plasmid is designed for use with the ExpreS2 Platform from ExpreS2ion Biotechnologies. It is intended to be used with the ExpreS2ion cells and media which can be obtained directly from the ExpreS2ion website.
Proper citation: RRID:Addgene_175446 Copy
Species: Homo sapiens
Genetic Insert: Gal1-p53
Vector Backbone Description: Backbone Size:4800; Vector Backbone:pRS314; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:7597081
Comments: RS314-SN contains wild type p53 driven by the Gal1 promoter (Gal1-p53).
Proper citation: RRID:Addgene_17539 Copy
Species: Synthetic
Genetic Insert: TVA-P2A-eGFP-P2A-N2cG
Vector Backbone Description: Backbone Marker:Jordane Dimidschstein (Addgene plasmid # 170853); Vector Backbone:pAAV-VTKS2; Vector Types:AAV, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34758329
Proper citation: RRID:Addgene_175439 Copy
Species: Homo sapiens
Genetic Insert: B-Raf
Vector Backbone Description: Backbone Size:5200; Vector Backbone:pBabe-puro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17699719
Proper citation: RRID:Addgene_17544 Copy
Vector Backbone Description: Backbone Marker:Genscript; Backbone Size:2686; Vector Backbone:pUC18; Vector Types:Mouse Targeting, Cloning vector, to be used to deliver transgene into mice with dual heterologous Bxb1 attP sites; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35361849
Comments: We recommend this plasmid for the generation of the DNA donor when the inserted transgene size is <15kb. For larger insertions, we recommend the low-copy plasmid p5155 (175391).
Depending on the nature of the sequence cloned into this plasmid, it may be beneficial to grow using NEB Stable, at 30C. However, just to maintain p5154 alone, DH5alpha is adequate (though we generally use DH10Beta).
Proper citation: RRID:Addgene_175390 Copy
Species: Mus musculus
Genetic Insert: Foxo1 LAALL
Vector Backbone Description: Backbone Size:4657; Vector Backbone:pCMV5; Vector Types:Mammalian Expression, RNAi; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_17548 Copy
Vector Backbone Description: Backbone Marker:Genscript; Backbone Size:4361; Vector Backbone:pBR322; Vector Types:Mouse Targeting, Cloning vector, to be used to deliver transgene into mice with dual heterologous Bxb1 attP sites; Bacterial Resistance:Kanamycin
Defining Citation: PMID:35361849
Comments: We recommend this low-copy plasmid for the generation of donor DNA when the inserted transgene size is >15kb. For smaller transgene insertions, we recommend the high-copy plasmid p5154 (Addgene 175390).
Proper citation: RRID:Addgene_175391 Copy
Species: Homo sapiens
Genetic Insert: EZR
Vector Backbone Description: Vector Backbone:pNIC28-Bsa4; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: Ligation-independent cloning 5' site GTACTTCCAATCCAT; 3' site TATCCACCTTTACT. Gileadi lab uses BL21(DE3)-R3-pRARE2 cells for expression.
Proper citation: RRID:Addgene_175461 Copy
Species: Homo sapiens
Genetic Insert: NAP1L1
Vector Backbone Description: Vector Backbone:pH6HTC_minus_cer-region; Vector Types:Bacterial Expression, Cell Free Expression; Bacterial Resistance:Ampicillin
Comments: Please visit https://doi.org/10.1101/2021.11.08.467743 for bioRxiv preprint.
Proper citation: RRID:Addgene_175342 Copy
Species: Homo sapiens
Genetic Insert: MDK
Vector Backbone Description: Vector Backbone:pHL-Sec; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: Gileadi lab uses HEK293 cells for expression.
Proper citation: RRID:Addgene_175464 Copy
Species: Homo sapiens
Genetic Insert: MSN
Vector Backbone Description: Vector Backbone:pNIC28-Bsa4; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: Ligation-independent cloning 5' site GTACTTCCAATCCAT; 3' site TATCCACCTTTACT. Gileadi lab uses BL21(DE3)-R3-pRARE2 cells for expression.
Proper citation: RRID:Addgene_175465 Copy
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