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Species: Mus musculus
Genetic Insert: CTIP2 isoform b
Vector Backbone Description: Backbone Marker:Homemade; Backbone Size:8465; Vector Backbone:N106; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26379228
Proper citation: RRID:Addgene_66808 Copy
Vector Backbone Description: Backbone Marker:Joachim Messing lab; Backbone Size:6403; Vector Backbone:pUC19; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:20472726
Proper citation: RRID:Addgene_66811 Copy
Species: Caenorhabditis elegans
Genetic Insert: TagRFP-T-C1^SEC^3xFlag
Vector Backbone Description: Backbone Size:2600; Vector Backbone:pUC19 (modified); Vector Types:Worm Expression, Cre/Lox, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26044593
Comments: THE DEPOSITING LAB NO LONGER USES THIS CONSTRUCT AND RECOMMENDS pDD286 (https://www.addgene.org/70684/). The full sequence for this plasmid can still be found by clicking the link next to "Addgene Sequences" and scrolling to the bottom of the page for "Full Addgene NGS sequence".
IMPORTANT NOTE: The repeat regions in this plasmid make it susceptible to recombination. The depositing lab grows the plasmid at 30°C. You may also want to prep and digest multiple single colonies to verify that you have a clone that has not recombined.
Proper citation: RRID:Addgene_66825 Copy
Species: Synthetic
Genetic Insert: Channelrhodopsin-2
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:5384; Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26443033
Proper citation: RRID:Addgene_66709 Copy
Genetic Insert: mCherry
Vector Backbone Description: Vector Backbone:components of pSie and FUGW; Vector Types:Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21611182
Proper citation: RRID:Addgene_66700 Copy
Species: Synthetic
Genetic Insert: Broccoli in the F30 scaffold
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5215; Vector Backbone:pET-28c; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:26000751
Proper citation: RRID:Addgene_66788 Copy
Genetic Insert: NleL
Vector Backbone Description: Vector Backbone:pMCSG17; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23563141
Proper citation: RRID:Addgene_66716 Copy
Species: Mus musculus
Genetic Insert: hbp
Vector Backbone Description: Backbone Marker:OPPF-UK; Backbone Size:5531; Vector Backbone:pOPINB; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25752577
Proper citation: RRID:Addgene_66712 Copy
Genetic Insert: e2-epf
Vector Backbone Description: Backbone Marker:GE Healthcare; Backbone Size:4984; Vector Backbone:pGEX6P1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20622874
Proper citation: RRID:Addgene_66713 Copy
Species: Synthetic
Genetic Insert: pTIGHT promoter-hM3Dq-mCherry
Vector Backbone Description: Backbone Size:3000; Vector Backbone:pUC; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25706476
Comments: hM3Dq-mCherry reading frame was obtained from pAAV-hSyn-DIO-hM3D(Gq)-mCherry (Addgene plasmid #44361) and cloned in the sense orientation between the lox sites. Please see the associated publication for additional cloning details.
Proper citation: RRID:Addgene_66795 Copy
Species: Aequorea victoria
Genetic Insert: GFP
Vector Backbone Description: Backbone Marker:Ian Frasier, Addgene Plasmid #25737; Backbone Size:13918; Vector Backbone:pSLIK-hygro; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24882210
Proper citation: RRID:Addgene_66844 Copy
Species: Synthetic
Genetic Insert: Two dimeric Broccoli aptamers in the F30 scaffold
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5215; Vector Backbone:pET-28c; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:26995352
Proper citation: RRID:Addgene_66843 Copy
Genetic Insert: mCherry-T2A-Cre
Vector Backbone Description: Vector Backbone:pRubi; Vector Types:Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25609613
Proper citation: RRID:Addgene_66692 Copy
Species: Homo sapiens
Genetic Insert: LATS2
Vector Backbone Description: Backbone Marker:Promega; Vector Backbone:pClneo; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21586534
Proper citation: RRID:Addgene_66852 Copy
Species: Mus musculus
Genetic Insert: Myb
Vector Backbone Description: Backbone Marker:Addgene Plasmid #18751; Backbone Size:6300; Vector Backbone:MSCV PIG; Vector Types:Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25982114
Proper citation: RRID:Addgene_66988 Copy
Species: Mus musculus
Genetic Insert: Myb
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25982114
Proper citation: RRID:Addgene_66980 Copy
Species: Xenopus laevis
Genetic Insert: His6-Thrombin-Xenopus laevis histones H2A
Vector Backbone Description: Backbone Size:5371; Vector Backbone:pET29a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:22617796
Proper citation: RRID:Addgene_66890 Copy
Species: Synthetic
Genetic Insert: LOC8R
Vector Backbone Description: Backbone Marker:GeneArt (LifeTechnologies); Backbone Size:2278; Vector Backbone:pMK-RQ (kanR); Vector Types:; Bacterial Resistance:Kanamycin
Comments: The LOC8R targeting sequence was designed by Benjamin S. Padman during his doctoral research project (Primary supervisor: Georg Ramm)
Insert into an N1 vector by subcloning. The number of Cox8 MTS repeats in LOC8R can be modified as follows:
4x Cox8: Insert at HindIII & AgeI sites. (subclone directly).
3x Cox8: Digest with BamHI and re-ligate. insert at HindIII & AgeI sites.
2x Cox8: Digest with XhoI and re-ligate. insert at HindIII & AgeI sites.
1x Cox8: Digest with HindIII and BamHI. Insert at HindIII & BamHI sites.
Proper citation: RRID:Addgene_66896 Copy
Species: Mus musculus
Genetic Insert: sgLkb1
Vector Backbone Description: Vector Backbone:pLL3.3; Vector Types:Mammalian Expression, Mouse Targeting, Lentiviral, Cre/Lox, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26178787
Proper citation: RRID:Addgene_66894 Copy
Species: Mus musculus
Genetic Insert: Erg
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25982114
Proper citation: RRID:Addgene_66977 Copy
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