Searching the RRID Resource Information Network
Are you sure you want to leave this community? Leaving the community will revoke any permissions you have been granted in this community.
| Plasmid Name | Proper Citation | Insert Name | Organism | Bacterial Resistance | Defining Citation |
Comments |
||||
|---|---|---|---|---|---|---|---|---|---|---|
|
pAWP78-PVCpnf_pvc13-A4DARPin Resource Report Resource Website 1+ mentions |
RRID:Addgene_198288 | PVCpnf_pvc13-A4DARPin | Photorhabdus asymbiotica | Kanamycin | PMID:36991127 | A446T mutation in pvc8 vgrG tail spike feature does not affect plasmid function. | Vector Backbone:pAWP78; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin | 2026-08-15 01:26:04 | 1 | |
|
pLenti-mCherry-CAAX PuroR Resource Report Resource Website 1+ mentions |
RRID:Addgene_166228 | mCherry-CAAX | Synthetic | Ampicillin | PMID:34407185 | Backbone Size:7602; Vector Backbone:LV 1-5; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin | 2026-08-15 01:26:03 | 1 | ||
|
pBR322-PVCpnf17-22 Resource Report Resource Website 1+ mentions |
RRID:Addgene_198272 | PVCpnf17-22 | Photorhabdus asymbiotica | Ampicillin | PMID:36991127 | Vector Backbone:pBR322; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:26:04 | 1 | ||
|
pLenti-hSyn1-TagBFP2/CAAX Resource Report Resource Website 1+ mentions |
RRID:Addgene_197590 | mTagBFP2-CAAX | Other | Ampicillin | Backbone Size:8408; Vector Backbone:pLenti; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin | 2026-08-15 01:26:05 | 1 | |||
|
pSBtet-Hygro-GADD34_Δ1-240/P2A/K3L Resource Report Resource Website 1+ mentions |
RRID:Addgene_196136 | GADD34 Δ1-240 - P2A - K3L | Homo sapiens | Ampicillin | PMID:36798401 | Backbone Size:7664; Vector Backbone:pSBtet-Hyg; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:26:06 | 2 | ||
|
pCAGGS-FLAG-BMAL1 Resource Report Resource Website 1+ mentions |
RRID:Addgene_186829 | bmal1 | Mus musculus | Ampicillin | PMID:35902736 | Vector Backbone:pCAGGS; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:26:07 | 1 | ||
|
pLKO.1 - TRC mTagBFP2-Synapsin1a Resource Report Resource Website 1+ mentions |
RRID:Addgene_191567 | Ampicillin | PMID:39167658 | Backbone Marker:TRC; Backbone Size:11079; Vector Backbone:PLKO.1; Vector Types:Mammalian Expression, Lentiviral, RNAi; Bacterial Resistance:Ampicillin | 2026-08-15 01:26:07 | 1 | ||||
|
pLX311-GFP-MEKDD Resource Report Resource Website 1+ mentions |
RRID:Addgene_194882 | MEKDD | Homo sapiens | Ampicillin | PMID:36991492 | Vector Backbone:pLX311-GFP; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin | S218D, S222D | 2026-08-15 01:26:05 | 1 | |
|
pET-21a(+)-BMTU UDG-6xHis Resource Report Resource Website 1+ mentions |
RRID:Addgene_172197 | BMTU UDG | marine bacterium BMTU 3346 UDG | Ampicillin | E. coli codon-optimised variant of BMTU 3346 UDG gene originally published in: Jaeger, S., Schmuck, R., & Sobek, H. (2000). Molecular cloning, sequency, and expression of the heat-labile uracil-DNA glycosylase from a marine psychrophilic bacterium, strain BMTU3346. Extremophiles : life under extreme conditions, 4(2), 115–122. https://doi.org/10.1007/s007920050145 | Backbone Marker:EMD Bioscience; Backbone Size:5443; Vector Backbone:pET-21(+); Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:26:07 | 1 | ||
|
PB-TO-oNGN2 Resource Report Resource Website 1+ mentions |
RRID:Addgene_198397 | NEUROG2 (phospho mutant, codon optimized) | Homo sapiens | Ampicillin | Backbone Size:13243; Vector Backbone:pUCM; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | S24A, S193A, S207A, S209A, S219A, S232A, S239A, S242A | 2026-08-15 01:26:07 | 1 | ||
|
p4928 Resource Report Resource Website 1+ mentions |
RRID:Addgene_168177 | PEM7::tagrfp-T PtetA::sfgfp | Synthetic | Ampicillin | PMID:33947954 | Please visit https://www.biorxiv.org/content/10.1101/2020.09.22.308114v1 for bioRxiv preprint. | Vector Backbone:pMW211; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | none | 2026-08-15 01:26:07 | 1 |
|
kiCAP-AAV-PAL1B Resource Report Resource Website 1+ mentions |
RRID:Addgene_196684 | AAV9 VP1 modified with 7mer insertion between amino acids 588 and 589 | Synthetic | Kanamycin | PMID:36417917 | Backbone Size:3000; Vector Backbone:pUC57-Kan; Vector Types:AAV; Bacterial Resistance:Kanamycin | PSQGTLR insert between amino acids 588 and 589 of AAV9 VP1 | 2026-08-15 01:26:07 | 1 | |
|
kiCAP-AAV-M.Fas.1 Resource Report Resource Website 1+ mentions |
RRID:Addgene_196687 | AAV9 VP1 modified with 7mer insertion between amino acids 588 and 589 | Synthetic | Kanamycin | PMID:36417917 | Backbone Size:3000; Vector Backbone:pUC57-Kan; Vector Types:AAV; Bacterial Resistance:Kanamycin | TDALTTK insert between amino acids 588 and 589 of AAV9 VP1 | 2026-08-15 01:26:07 | 1 | |
|
kiCAP-AAV-M.Mus.1 Resource Report Resource Website 1+ mentions |
RRID:Addgene_196681 | AAV9 VP1 modified with 7mer insertion between amino acids 588 and 589 | Synthetic | Kanamycin | PMID:36417917 | Backbone Size:3000; Vector Backbone:pUC57-Kan; Vector Types:AAV; Bacterial Resistance:Kanamycin | WVLPSGG insert between amino acids 588 and 589 of AAV9 VP1 | 2026-08-15 01:26:08 | 1 | |
|
kiCAP-AAV-PAL2 Resource Report Resource Website 1+ mentions |
RRID:Addgene_196691 | AAV9 VP1 modified with 7mer insertion between amino acids 588 and 589 | Synthetic | Kanamycin | PMID:36417917 | Backbone Size:3000; Vector Backbone:pUC57-Kan; Vector Types:AAV; Bacterial Resistance:Kanamycin | PTQGTVR insert between amino acids 588 and 589 of AAV9 VP1, S586E, A587V, Q588G | 2026-08-15 01:26:07 | 1 | |
|
VR122 pETv2 10His-pG-Tn5 (E54K, L372P) Resource Report Resource Website 1+ mentions |
RRID:Addgene_198467 | 10His-pG-Tn5 (E54K, L372P) | Synthetic | Kanamycin | PMID:39026714 | Please visit https://www.biorxiv.org/content/10.1101/2024.07.11.602973v1 for bioRxiv preprint. | Backbone Size:5234; Vector Backbone:pETv2; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin | E54K, L372P | 2026-08-15 01:26:09 | 1 |
|
lentiGuide-Puro.3xBsmBI Resource Report Resource Website 1+ mentions |
RRID:Addgene_196709 | Ampicillin | PMID:28993443 | Backbone Marker:Feng Zhang Lab; Vector Backbone:lentiGuide-Puro (#52963); Vector Types:Lentiviral, CRISPR; Bacterial Resistance:Ampicillin | 2026-08-15 01:26:08 | 6 | ||||
|
pUS250 Resource Report Resource Website 1+ mentions |
RRID:Addgene_198322 | Kanamycin | CLONING: The multiple cloning site (MCS) in pUS250 functions differently to a typical MCS. You need to choose one unique restriction site on the left side of the amilCP marker gene (e.g. EcoRI), and one unique restriction site on the right side (e.g. PstI) in order for the blue/white selection and the cumate-inducible expression features to work properly. Cutting the plasmid in this way excises the amilCP gene, replacing it with your gene of interest, and changing the phenotype from blue to white. The vector is also compatible with GoldenGate cloning (using either BsaI or Esp3I) and BioBrick cloning (iGEM). In the case of GoldenGate cloning, you don't need to use two different enzymes, you just use one or the other, since each enzyme has two sites, one on each side of amilCP, and each yields a different overhang. HOST RANGE and EXPRESSION: we have shown that pUS250 can be used for cumate-inducible gene expression in E.coli, Pseudomonas putida, and Rhizobium leguminosarum. It is likely that the plasmid will also be useful in other gram negatives (Proteobacteria) since the pBBR replicon has a very broad host range. The plasmid can be transferred by conjugation from E.coli strains such as S17 or SM10 into other species due to the oriT sequence. For the E.coli and Rhizobium, 100 uM cumate is sufficient for expression. For Pseudomonas, this needs to be increased to 10 mM (we think there is an efflux pump that pumps cumate back out of these cells). The cumate should be added after autoclaving. We make a 0.5 M cumate stock solution by mixing equal parts of 1M aqueous Tris base with 1M cumic acid in ethanol. Cumate is an excellent inducer since it is both cheap and non-toxic to both bacteria and people. You can even use cumin (the spice) for induction of gene expression in this plasmid! (there is enough cumate in the cumin). STABILITY and COPY NUMBER: We estimate that the vector has a copy number of 5-10 in E.coli, so it is certainly a low copy vector. Best to make large-scale plasmid preps (e.g. 50 ml culture) rather than small-scale, in order to ensure you get enough plasmid DNA to work with. The plasmid is quite stable but we have seen white mutants appear occasionally which have deletions in amilCP. | Backbone Marker:Coleman; Backbone Size:4690; Vector Backbone:pUS250; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Kanamycin | 2026-08-15 01:26:09 | 2 | ||||
|
lenti-Cas9-sgHPRT1 Resource Report Resource Website 1+ mentions |
RRID:Addgene_196713 | Cas9-T2A-BSD-U6-sgHPRT1 | S. pyogenes | Ampicillin | PMID:28993443 | Backbone Marker:Feng Zhang Lab; Vector Backbone:lenti dCas9-VP64_Blast (#61425); Vector Types:Lentiviral, CRISPR; Bacterial Resistance:Ampicillin | 2026-08-15 01:26:09 | 2 | ||
|
lenti-dCas9-ZIM3-KRAB-BFP Resource Report Resource Website 1+ mentions |
RRID:Addgene_196712 | dCas9-KRAB(ZIM3)-T2A-TagBFP | S. pyogenes | Ampicillin | Backbone Marker:Feng Zhang Lab; Vector Backbone:lenti dCas9-VP64_Blast (#61425); Vector Types:Lentiviral, CRISPR; Bacterial Resistance:Ampicillin | D10A, N863A | 2026-08-15 01:26:08 | 1 |
Can't find your Plasmid?
We recommend that you click next to the search bar to check some helpful tips on searches and refine your search firstly. If you want to find a specific plasmid, it's easier to enter an RRID or an Addgene Catalog Number to search. You can refine the search results using Facets on the left side of the search results page. If you are on the table view, you can also search in a specific column by clicking the column title and enter the keywords.
If you still could not find your plasmid in the search results, please help us by registering it into the system — it's easy. Register it with Addgene.
Welcome to the RRID Resources search. From here you can search through a compilation of resources used by RRID and see how data is organized within our community.
You are currently on the Community Resources tab looking through categories and sources that RRID has compiled. You can navigate through those categories from here or change to a different tab to execute your search through. Each tab gives a different perspective on data.
If you have an account on RRID then you can log in from here to get additional features in RRID such as Collections, Saved Searches, and managing Resources.
Here is the search term that is being executed, you can type in anything you want to search for. Some tips to help searching:
If you are logged into RRID you can add data records to your collections to create custom spreadsheets across multiple sources of data.
Here are the facets that you can filter the data by.
If you have any further questions please check out our FAQs Page to ask questions and see our tutorials. Click this button to view this tutorial again.
