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Species: Danio rerio
Genetic Insert: Sp5l promoter
Vector Backbone Description: Backbone Marker:promega; Backbone Size:4818; Vector Backbone:pGL3-Basic; Vector Types:Luciferase; Bacterial Resistance:Ampicillin
Comments: Description: a fragment of putative zebrafish Sp5l promoter - 1203bp upstream of the 1st Exon.
(as defined by the 27i2 clone found in the microarray screen) - plus 51bp of 5'UTR from Exon1 clone.
upstream of firefly luciferase in the pGL3 Basic promoter. 6 of the putative Tcf/Lef sites mutated.
(all introduce additional restriction sites); cloned by PCR-based in vitro synthesis from 28 overlapping.
oligos. cloned NdeI / HindIII to exchange wild-type fragment in pGL3 Sp5l P1203.
Species of Sequence Origin: zebrafish.
Proper citation: RRID:Addgene_17209 Copy
Species: Synthetic
Genetic Insert: PT7/Lac-[His6-TEV-SUMO-PylB.JM10.1deopt]
Vector Backbone Description: Vector Backbone:pBR322; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34168131
Proper citation: RRID:Addgene_172177 Copy
Species: Synthetic
Genetic Insert: PT7/Lac-[His6-TEV-SUMO-PylB.WTopt]
Vector Backbone Description: Vector Backbone:pBR322; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34168131
Proper citation: RRID:Addgene_172175 Copy
Species: Synthetic
Genetic Insert: PT7/Lac-[His6-TEV-SUMO-PylB.JM10.1]
Vector Backbone Description: Vector Backbone:pBR322; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34168131
Proper citation: RRID:Addgene_172174 Copy
Species: Synthetic
Genetic Insert: Pem7 luxCDA(B.1TAG)E; Plpp pylS; PproK pylT
Vector Backbone Description: Vector Backbone:p15A; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:34168131
Proper citation: RRID:Addgene_172169 Copy
Species: Synthetic
Genetic Insert: Para (pylBCD 3f2+C); AraC
Vector Backbone Description: Vector Backbone:pBR322; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34168131
Proper citation: RRID:Addgene_172165 Copy
Species: Synthetic
Genetic Insert: Para (pylBCD 3f2v2+B); AraC
Vector Backbone Description: Vector Backbone:pBR322; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34168131
Proper citation: RRID:Addgene_172164 Copy
Species: Homo sapiens
Genetic Insert: CD63
Vector Backbone Description: Backbone Size:9331; Vector Backbone:pLenti-CMV Blast DEST (706-1); Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32350252
Proper citation: RRID:Addgene_172117 Copy
Species: Homo sapiens
Genetic Insert: hNGN2
Vector Backbone Description: Backbone Size:13245; Vector Backbone:pUCM; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36459969
Comments: This plasmid has been found to run as a multimer. Plasmid multimerization often does not impact plasmid function, but may reduce transformation efficiencies. You may need to screen multiple colonies to isolate the monomeric version of this plasmid. If you still have trouble isolating the monomeric version, you might consider linearizing, gel extracting, re-ligating, and transforming the plasmid.
Proper citation: RRID:Addgene_172115 Copy
Species: N.Neisseria meningitidis serogroup B (strain MC58)
Genetic Insert: MetN
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5245; Vector Backbone:pET21b+; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34409939
Proper citation: RRID:Addgene_172114 Copy
Species: Homo sapiens
Genetic Insert: hNGN2
Vector Backbone Description: Backbone Size:12000; Vector Backbone:pUCM; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36459969
Proper citation: RRID:Addgene_172113 Copy
Species: Homo sapiens
Genetic Insert: EIF2B3
Vector Backbone Description: Backbone Size:4000; Vector Backbone:pCS2+; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33517449
Proper citation: RRID:Addgene_172078 Copy
Species: Homo sapiens
Genetic Insert: EIF2B3
Vector Backbone Description: Backbone Size:4000; Vector Backbone:pCS2+; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33517449
Proper citation: RRID:Addgene_172077 Copy
Species: Homo sapiens
Genetic Insert: EIF2B3
Vector Backbone Description: Backbone Size:4000; Vector Backbone:pCS2+; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33517449
Proper citation: RRID:Addgene_172076 Copy
Species: Homo sapiens
Genetic Insert: EIF2B3
Vector Backbone Description: Backbone Size:4000; Vector Backbone:pCS2+; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33517449
Proper citation: RRID:Addgene_172074 Copy
Species: Synthetic
Genetic Insert: mCherry
Vector Backbone Description: Backbone Marker:Yiping Qi; Backbone Size:16112; Vector Backbone:A844B; Vector Types:Plant Expression, CRISPR; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34713243
Comments: Please note that the Addgene verified sequence differs from the depositor reference sequence. These discrepancies do not affect plasmid function.
Proper citation: RRID:Addgene_172182 Copy
Species: Homo sapiens
Genetic Insert: KLF4
Vector Backbone Description: Backbone Size:4600; Vector Backbone:pMXs; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18035408
Comments: The sequence of primer pMX-S1811 is GAC GGC ATC GCA GCT TGG ATA CAC.
Proper citation: RRID:Addgene_17219 Copy
Species: Homo sapiens
Genetic Insert: c-MYC
Vector Backbone Description: Backbone Size:4600; Vector Backbone:pMXs; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18035408
Comments: The sequence of primer pMX-S1811 is GAC GGC ATC GCA GCT TGG ATA CAC.
Proper citation: RRID:Addgene_17220 Copy
Species: Homo sapiens
Genetic Insert: Upstream region of human REX1
Vector Backbone Description: Backbone Size:4842; Vector Backbone:pGV-BM2; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18035408
Proper citation: RRID:Addgene_17222 Copy
Species: Mus musculus
Genetic Insert: PolII promoter
Vector Backbone Description: Backbone Size:4842; Vector Backbone:pGV-BM2; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18035408
Proper citation: RRID:Addgene_17223 Copy
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