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Species: Synthetic
Genetic Insert: pSEVA-331Bb-11AC-GFP
Vector Backbone Description: Vector Backbone:pSEVA-331Bb; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:30865830
Comments: High expression
Proper citation: RRID:Addgene_138047 Copy
Species: Synthetic
Genetic Insert: pSEVA-331Bb-12AG-GFP
Vector Backbone Description: Vector Backbone:pSEVA-331Bb; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:30865830
Comments: High expression
Proper citation: RRID:Addgene_138049 Copy
Species: Synthetic
Genetic Insert: pSEVA-331Bb-12AC-GFP
Vector Backbone Description: Vector Backbone:pSEVA-331Bb; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:30865830
Comments: High expression
Proper citation: RRID:Addgene_138050 Copy
Species: Synthetic
Genetic Insert: pSEVA-331Bb-WTRBS-GFP
Vector Backbone Description: Vector Backbone:pSEVA-331Bb; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:30865830
Comments: Intermediate expression
Proper citation: RRID:Addgene_138052 Copy
Species: Synthetic
Genetic Insert: pSEVA-331Bb-1AG 2AC-GFP
Vector Backbone Description: Vector Backbone:pSEVA-331Bb; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:30865830
Comments: High expression
Proper citation: RRID:Addgene_138054 Copy
Species: Synthetic
Genetic Insert: pSEVA-331Bb-1AG 11AC-GFP
Vector Backbone Description: Vector Backbone:pSEVA-331Bb; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:30865830
Comments: High expression
Proper citation: RRID:Addgene_138056 Copy
Species: Synthetic
Genetic Insert: pSEVA-331Bb- 2AC 11AC-GFP
Vector Backbone Description: Vector Backbone:pSEVA-331Bb; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:30865830
Comments: High expression
Proper citation: RRID:Addgene_138055 Copy
Species: Synthetic
Genetic Insert: J23108- pSEVA-331Bb- RFP
Vector Backbone Description: Vector Backbone:pSEVA-331Bb; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:30865830
Comments: Low expression
Proper citation: RRID:Addgene_138061 Copy
Species: Synthetic
Genetic Insert: J23105- pSEVA-331Bb- RFP
Vector Backbone Description: Vector Backbone:pSEVA-331Bb; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:30865830
Comments: Low expression
Proper citation: RRID:Addgene_138060 Copy
Species: Synthetic
Genetic Insert: APOBEC3A-wtTadA-TadA7.10-nCas9-UGI-NLS
Vector Backbone Description: Vector Backbone:pJIT163; Vector Types:Plant Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_138137 Copy
Species: Synthetic
Genetic Insert: APOBEC3A-wtTadA-TadA7.10-nCas9-2X(T2A-UGI-NLS)
Vector Backbone Description: Vector Backbone:pJIT163; Vector Types:Plant Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_138139 Copy
Species: Synthetic
Genetic Insert: wtTadA-TadA7.10-APOBEC3A-nCas9-UGI-NLS
Vector Backbone Description: Vector Backbone:pJIT163; Vector Types:Plant Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_138138 Copy
Species: Synthetic
Genetic Insert: wtTadA-TadA7.10-nCas9-NG-3XNLS
Vector Backbone Description: Vector Backbone:pJIT163; Vector Types:Plant Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_138142 Copy
Species: Synthetic
Genetic Insert: PKAsub-YPet-Kras
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5472; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21438554
Proper citation: RRID:Addgene_138206 Copy
Species: Synthetic
Genetic Insert: mouse Dnmt1 sgRNA
Vector Backbone Description: Backbone Marker:Zhang lab; Vector Backbone:lentiCRISPR v2; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31209294
Proper citation: RRID:Addgene_138348 Copy
Species: Synthetic
Genetic Insert: CBX1mut-Dronpa
Vector Backbone Description: Backbone Size:5523; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30417094
Proper citation: RRID:Addgene_138250 Copy
Species: Synthetic
Genetic Insert: GFP targeting sgRNAs G and H
Vector Backbone Description: Vector Backbone:pRS424; Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31436506
Proper citation: RRID:Addgene_138257 Copy
Species: Synthetic
Genetic Insert: eGFP
Vector Backbone Description: Vector Backbone:pSB1C3; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:31436506
Proper citation: RRID:Addgene_138261 Copy
Species: Synthetic
Genetic Insert: Nontargeting sgRNA
Vector Backbone Description: Vector Backbone:pUC19; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31436506
Proper citation: RRID:Addgene_138260 Copy
Species: Synthetic
Genetic Insert: Neo*
Vector Backbone Description: Backbone Marker:Stratagene; Vector Backbone:pBluescript; Vector Types:Mammalian Expression, Bacterial Expression, Mouse Targeting, att/PhiC31; Bacterial Resistance:Ampicillin
Comments: The FRT sites in plasmid pFNF #22687 were replaced by attB and attP sites.
The Neo* coding sequence has an E to D mutation at residue 185 corresponding to the original residue 182 (see Yenofsky et al., 1990, https://doi.org/10.1073/pnas.87.9.3435). Its reduced activity could mean that the cell would have to have higher level of the mutant protein expressed in order to achieve drug resistance at the same G418 dose. This could be relevant to mouse embryonic stem cell gene targeting efficiency (also see Hanson and Sedivy, 1995, https://doi.org/10.1128/mcb.15.1.45).
Proper citation: RRID:Addgene_138412 Copy
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