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Genetic Insert: Green fluorescent protein
Vector Backbone Description: Vector Backbone:pET; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25386923
Proper citation: RRID:Addgene_61328 Copy
Genetic Insert: Green fluorescent protein
Vector Backbone Description: Vector Backbone:pET; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25386923
Proper citation: RRID:Addgene_61327 Copy
Species: Homo sapiens
Genetic Insert: FK506 binding protein 1A
Vector Backbone Description: Vector Backbone:C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25892241
Comments: T389E in AIP (in-frame with) FKBP caused by GA mismatch at 1180 bp, AC mismatch at 1181 bp, and AT mismatch at 1182 bp in Author's partial sequence (please refer to mChF-AIP for comparison).
Proper citation: RRID:Addgene_61530 Copy
Species: Synthetic
Genetic Insert: F2620-rbs33-hrpR-ter
Vector Backbone Description: Backbone Marker:BioBrick registry; Backbone Size:2750; Vector Backbone:pSB3K3; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:22009040
Proper citation: RRID:Addgene_61436 Copy
Species: Synthetic
Genetic Insert: ntdTomato
Vector Backbone Description: Backbone Size:2580; Vector Backbone:pGreenII; Vector Types:Plant Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25643149
Comments: Please note that Addgene NGS results identified as stop codon after the second EGFP ORF. This stop codon was present in the original, published version of the plasmid, such that the insert is n2GFP rather than n3GFP. The plasmid functions as described in the associated publication. Co-expression with the helper plasmid pSOUP is suggested for expression in Agrobacterium.
The diagnostic digest indicates this plasmid may be bigger than the assembled sequence suggests.
Proper citation: RRID:Addgene_61628 Copy
Species: Homo sapiens
Genetic Insert: Cezanne2
Vector Backbone Description: Backbone Marker:OPPF-UK; Backbone Size:6299; Vector Backbone:pOPINK; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:23827681
Comments: This construct is the Isoform 2 of Cezanne2.
Proper citation: RRID:Addgene_61582 Copy
Genetic Insert: mEGFP-30nmSAH-IQ
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4013; Vector Backbone:C1; Vector Types:; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25365993
Proper citation: RRID:Addgene_61678 Copy
Genetic Insert: mEGFP-EF(N)-30nmSAH
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4013; Vector Backbone:C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25365993
Proper citation: RRID:Addgene_61679 Copy
Species: Rattus norvegicus
Genetic Insert: KIF5C(1-560)-EF(C)
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4013; Vector Backbone:C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25365993
Proper citation: RRID:Addgene_61675 Copy
Vector Backbone Description: Backbone Size:4658; Vector Backbone:pBAMD1-2; Vector Types:Synthetic Biology, Mini-Tn5 vector; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25389526
Comments: This plasmid has been found to be somewhat unstable and prone to concatenation. Concatenation often does not impact plasmid function, but may reduce transformation efficiencies. You may need to screen multiple colonies to isolate the monomeric version of this plasmid. If you still have trouble isolating the monomeric version, you might consider linearizing, gel extracting, re-ligating, and transforming the plasmid.
Proper citation: RRID:Addgene_61564 Copy
Genetic Insert: LSSmOrange and CNA35
Vector Backbone Description: Backbone Marker:Novagen; Vector Backbone:pET28a; Vector Types:; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25490719
Proper citation: RRID:Addgene_61605 Copy
Species: Nostoc punctiforme
Genetic Insert: NpuDnaEN35 intein
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:3559; Vector Backbone:pRSFDuet; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:24574026
Proper citation: RRID:Addgene_61755 Copy
Species: Thermoplasma volcanium
Genetic Insert: TvoVMAdC48 intein
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:3559; Vector Backbone:pRSFDuet; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:24574026
Proper citation: RRID:Addgene_61759 Copy
Species: Homo sapiens
Genetic Insert: USP5
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5930; Vector Backbone:PET42C(+); Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:24424410
Proper citation: RRID:Addgene_61750 Copy
Species: Streptomyces hygroscopicus
Genetic Insert: Bar gene
Vector Backbone Description: Backbone Marker:CAMBIA; Backbone Size:6812; Vector Backbone:pCAMBIA-0380; Vector Types:Ternary vector for Agrobacterium mediated transformation of fungi; Bacterial Resistance:Kanamycin
Defining Citation: PMID:26092796
Comments: Jason Rudd, (Rothamsted research UK) provided Agrobacterium tumfaciens vector pCAMB-BAR (pCAMBIA-0380 backbone with bar gene under Neurospora crassa trpC promoter) For more information see: Kramer et al.. Fungal Genetics and Biology, 46, 667-681. https://www.ncbi.nlm.nih.gov/pubmed/19520179.
Bar gene from pCAMB-BAR was recombined into pC-HYG-YR (Addgene plasmid# 61765) through yeast recombineering to replace hygromycin gene and create pC-BAR-YR.
Restriction sites EcoRI and HindIII can be used to linearise the vector inside left (LB) and right (RB) borders of the TDNA. Note that the trpC promoter and bar sequences are present within the EcoRI and HindII cloning sites.
See Supplementary Fig. S1. of associated publication for schematic of single step assembly of gene deletion ternary vectors using yeast recombinational cloning.
Proper citation: RRID:Addgene_61766 Copy
Species: E. coli
Genetic Insert: nptII gene
Vector Backbone Description: Backbone Marker:CAMBIA; Backbone Size:6812; Vector Backbone:pCAMBIA-0380; Vector Types:Ternary vector for Agrobacterium mediated transformation of fungi; Bacterial Resistance:Kanamycin
Defining Citation: PMID:26092796
Comments: Jason Rudd, (Rothamsted research UK) provided Agrobacterium tumfaciens vector pCGEN (pCAMBIA-0380 backbone with nptII gene under Neurospora crassa gpd promoter).
NptII (neomycin phosphotransferase II) gene from pCGEN was recombined into pC-HYG-YR (Addgene plasmid# 61765) through yeast recombineering to replace hygromycin gene and create pC-G418-YR.
Restriction sites EcoRI and HindIII can be used to linearise the vector inside left (LB) and right (RB) borders of the TDNA. Note that the trpC promoter and nptII sequences are present within the EcoRI and HindII cloning sites.
See Supplementary Fig. S1. of associated publication for schematic of single step assembly of gene deletion ternary vectors using yeast recombinational cloning.
Please note that the Addgene verified sequence identified a transposase in the backbone (7145-8353 bp).
Proper citation: RRID:Addgene_61767 Copy
Species: Synthetic
Genetic Insert: Hygromycin
Vector Backbone Description: Backbone Marker:CAMBIA; Backbone Size:6812; Vector Backbone:pCAMBIA-0380; Vector Types:Ternary vector for Agrobacterium mediated transformation of fungi; Bacterial Resistance:Kanamycin
Defining Citation: PMID:26092796
Comments: Jason Rudd (Rothamsted research UK) provided Agrobacterium tumfaciens vector pCHYG. For more information, see: Motteram et al, 2009. Molecular Plant-Microbe Interactions, 22, 790-799. https://www.ncbi.nlm.nih.gov/pubmed/19522561.
Restriction sites EcoRI and HindIII can be used to linearise the vector inside left (LB) and right (RB) borders of the TDNA. Note that the trpC promoter and hygromycin sequences are present within the EcoRI and HindII cloning sites.
See Supplementary Fig. S1. of associated publication (Sidhu et al., 2015) for schematic of single step assembly of gene deletion ternary vectors using yeast recombinational cloning.
Proper citation: RRID:Addgene_61765 Copy
Species: Thermoplasma volcanium
Genetic Insert: TvoVMAdC7 intein
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:3559; Vector Backbone:pRSFDuet; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:24574026
Proper citation: RRID:Addgene_61761 Copy
Species: Homo sapiens
Genetic Insert: OMA1
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:4300; Vector Backbone:pCMV-Tag4; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_61719 Copy
Species: túngara frog (Engystomops pustulosus)
Genetic Insert: Ranaspumin-2
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5369; Vector Backbone:pET-28; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:19527658
Proper citation: RRID:Addgene_61686 Copy
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