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Species: Caenorhabditis elegans
Genetic Insert: [AWAp | wrmScarlet | tbb-2 UTR]
Vector Backbone Description: Backbone Marker:Twist Biosciences; Vector Backbone:Twist Amp High; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Comments: See www.wormbuilder.org/BioParts for more information.
Proper citation: RRID:Addgene_200353 Copy
Species: Synthetic
Genetic Insert: T0 terminator - Sm resistance cassette
Vector Backbone Description: Backbone Size:4948; Vector Backbone:pSH624-ssr; Vector Types:Bacterial Expression; Bacterial Resistance:Streptomycin
Defining Citation: PMID:37798358
Proper citation: RRID:Addgene_198026 Copy
Species: Homo sapiens
Genetic Insert: matrix AAA peptidase interacting protein 1
Vector Backbone Description: Backbone Size:5339; Vector Backbone:CSII-TK-MCS; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37355744
Proper citation: RRID:Addgene_203599 Copy
Species: Synthetic
Genetic Insert: mNeonGreen
Vector Backbone Description: Vector Backbone:pMflT-o4; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Tetracycline
Comments: The user should always streak the culture on plate and work with isolated fluorescent colonies. Addgene NGS results found mixed population of nucleotides across Term_apFAB390 and C-terminus of NeonGreen, but plasmid function should not be affected.
Proper citation: RRID:Addgene_201855 Copy
Species: Dictyostelium discoideum
Genetic Insert: tandem 2xPX domain of SnxA
Vector Backbone Description: Backbone Size:7964; Vector Backbone:pJSK1045; Vector Types:Dictyostelium expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37382666
Comments: Please note: We have identified several potential errors in the highly non-complex and repetitive published sequence of the Dictyostelium pDM vector backbone. These are all in non-coding highly repetitive regions so likely not important for function.
Proper citation: RRID:Addgene_205133 Copy
Species: Synthetic
Genetic Insert: 2xFYVE (from human Hrs)
Vector Backbone Description: Backbone Size:7805; Vector Backbone:pDM448; Vector Types:Dictyostelium expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37382666
Comments: Please note: We have identified several potential errors in the highly non-complex and repetitive published sequence of the Dictyostelium pDM vector backbone. These are all in non-coding highly repetitive regions so likely not important for function.
Proper citation: RRID:Addgene_205131 Copy
Species: Triticum aestivum
Genetic Insert: pDefence14:Eluc
Vector Backbone Description: Vector Backbone:pICH47732; Vector Types:Plant Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_201330 Copy
Vector Backbone Description: Backbone Marker:RIKEN-BRC; Backbone Size:9136; Vector Backbone:CSII-EF-MCS; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37355744
Proper citation: RRID:Addgene_203597 Copy
Species: Pseudomonas putida DOT-T1E
Genetic Insert: single-stranded DNA recombineering
Vector Backbone Description: Backbone Marker:SEVA collection; Backbone Size:5089; Vector Backbone:pSEVA624; Vector Types:Bacterial Expression; Bacterial Resistance:Gentamicin
Defining Citation: PMID:37798358
Proper citation: RRID:Addgene_199241 Copy
Species: Synthetic
Genetic Insert: modified MCS
Vector Backbone Description: Backbone Marker:Robertson; Backbone Size:8072; Vector Backbone:pWR88; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37345937
Proper citation: RRID:Addgene_203916 Copy
Species: Mus musculus
Genetic Insert: Oct4
Vector Backbone Description: Vector Backbone:pMSCV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_206919 Copy
Species: Mus musculus
Genetic Insert: Oct4
Vector Backbone Description: Vector Backbone:pMSCV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_206918 Copy
Species: Mus musculus
Genetic Insert: Oct4
Vector Backbone Description: Vector Backbone:pMSCV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_206921 Copy
Species: Synthetic
Genetic Insert: mGreenLantern
Vector Backbone Description: Vector Backbone:pAAV; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37676945
Proper citation: RRID:Addgene_207613 Copy
Species: Mus musculus
Genetic Insert: Oct4
Vector Backbone Description: Vector Backbone:pMSCV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_206920 Copy
Species: Synthetic
Genetic Insert: photocaged SpyCatcher-LPETG
Vector Backbone Description: Backbone Size:5107; Vector Backbone:pET-21; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37069270
Proper citation: RRID:Addgene_207629 Copy
Species: Synthetic
Genetic Insert: mito-mCherryEA
Vector Backbone Description: Backbone Size:5273; Vector Backbone:GW1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30197999
Proper citation: RRID:Addgene_207868 Copy
Species: L. lactis
Genetic Insert: OppA
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2761; Vector Backbone:pRset; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30842982
Proper citation: RRID:Addgene_207869 Copy
Species: Synthetic
Genetic Insert: 8xTEAD-luc2
Vector Backbone Description: Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37813295
Proper citation: RRID:Addgene_173005 Copy
Species: Synthetic
Genetic Insert: CspRecT / Bxb-1
Vector Backbone Description: Vector Backbone:pKM461; Vector Types:Synthetic Biology; Bacterial Resistance:Gentamicin
Comments: Replicating ORBIT V2 helper plasmid w/ inducible oligo recombineering module (m-tol) and bxb-1 integrase (arabinose). SacB is present for counterselection and gentamicin is the resistance marker. V2 plasmid does not have mutL, which lowers background mutation rate. Please visit https://doi.org/10.1101/2023.06.28.546561 for bioRxiv preprint.
Proper citation: RRID:Addgene_205293 Copy
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