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Species: Mus musculus
Genetic Insert: Myf5
Vector Backbone Description: Backbone Size:0; Vector Backbone:Slax; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15634692
Comments: Full length RT-PCR product.
Proper citation: RRID:Addgene_13913 Copy
Species: Mus musculus
Genetic Insert: Pea3
Vector Backbone Description: Backbone Size:11600; Vector Backbone:RCASBP(A); Vector Types:Retroviral, avian expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15253939
Comments: Pea3 ETS domain fused to EnR.
Proper citation: RRID:Addgene_13909 Copy
Species: Mus musculus
Genetic Insert: Pea3
Vector Backbone Description: Backbone Size:11600; Vector Backbone:RCASBP(A); Vector Types:Retroviral, avian expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15253939
Comments: Full-length Pea3.
Proper citation: RRID:Addgene_13908 Copy
Species: Mus musculus
Genetic Insert: Id1
Vector Backbone Description: Backbone Marker:Sen Wu, Capecchi laboratory; Vector Backbone:pWS-TK6; Vector Types:Mammalian Expression, Mouse Targeting, Cre/Lox; Bacterial Resistance:Ampicillin
Comments: Linker between sfGFP and iCre-ERT2 is a mutated P2A sequence where 2 conserved amino acids are changed to Alanines. This mutated sequence does not skip.
The sfGFP fluorescence from the sfGFP-iCre-ERT2 is dim, as described in Nam and Capecchi, 2020 (https://doi.org/10.1186/s13064-020-00139-5) and a Figshare page (https://doi.org/10.6084/m9.figshare.13218437.v35). Thus, the sfGFP fluorescence should be detectable only in cells in which the marker gene expression level is high.
Homology arms subcloned by recombineering from a clone of RPCI-23 C57BL/6J mouse genomic DNA library.
Please make sure the plasmid DNA preparation is pure by gel electrophoresis before linearizing for an electroporation.
Proper citation: RRID:Addgene_139279 Copy
Species: Mus musculus
Genetic Insert: Id1
Vector Backbone Description: Backbone Marker:Sen Wu, Capecchi laboratory; Vector Backbone:pWS-TK6; Vector Types:Mammalian Expression, Mouse Targeting; Bacterial Resistance:Ampicillin
Comments: Homology arms subcloned by recombineering from a clone of RPCI-23 C57BL/6J mouse genomic DNA library.
Please make sure the plasmid DNA preparation is pure by gel electrophoresis before linearizing for an electroporation.
Please note there are a few ambiguous bases in Addgene’s NGS result. This is due to repetitive A-G region. Depositor confirms these bases do not affect plasmid function.
Proper citation: RRID:Addgene_139281 Copy
Species: Mus musculus
Genetic Insert: Nf2 delta BB
Vector Backbone Description: Backbone Size:5169; Vector Backbone:pBabe-puro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12203111
Proper citation: RRID:Addgene_14117 Copy
Species: Mus musculus
Genetic Insert: Nf2
Vector Backbone Description: Backbone Size:5169; Vector Backbone:pBabe-puro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12203111
Proper citation: RRID:Addgene_14116 Copy
Species: Mus musculus
Genetic Insert: Mouse Beta 1 Integrin
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:2865; Vector Backbone:pGEM 1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_14127 Copy
Species: Mus musculus
Genetic Insert: gRNAs targeting mouse Solute Carrier genes
Vector Backbone Description: Backbone Marker:Feng Zhang lab (Addgene plasmid #52961); Vector Backbone:lentiCRISPR v2; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_141436 Copy
Species: Mus musculus
Genetic Insert: Cdk6
Vector Backbone Description: Backbone Marker:Sen Wu, Capecchi laboratory; Vector Backbone:pWS-TK6; Vector Types:Mammalian Expression, Mouse Targeting, dre / rox; Bacterial Resistance:Ampicillin
Comments: Linker between TagRFP-T and dre-PBD* is a mutated P2A sequence where 2 conserved amino acids are changed to Alanines. This mutated sequence does not skip.
The TagRFP-T fluorescence from the TagRFP-T-dre-PBD* is dim, presumably for the same reason as the sfGFP-iCre-ERT2 is dim, as described in Nam and Capecchi, 2020 (https://doi.org/10.1186/s13064-020-00139-5) and a Figshare page (https://doi.org/10.6084/m9.figshare.13218437.v35). Thus, the TagRFP-T fluorescence should be detectable only in cells in which the marker gene expression level is high.
Homology arms subcloned by recombineering from a clone of RPCI-24 C57BL/6J mouse genomic DNA library.
Please make sure the plasmid DNA preparation is pure by gel electrophoresis before linearizing for an electroporation.
Proper citation: RRID:Addgene_141452 Copy
Species: Mus musculus
Genetic Insert: PGC-1 alpha
Vector Backbone Description: Backbone Size:9000; Vector Backbone:pAdTrack-CMV; Vector Types:Mammalian Expression, Adenoviral; Bacterial Resistance:Kanamycin
Defining Citation: PMID:16753578
Comments: pAdTrack can be recombined with pAdEasy to make adenoviral-PGC1.
See He et al. http://www.pnas.org/cgi/content/full/95/5/2509 for protocols.
Proper citation: RRID:Addgene_14427 Copy
Species: Mus musculus
Genetic Insert: PGC-1 alpha
Vector Backbone Description: Backbone Size:9000; Vector Backbone:pAdTrack-CMV; Vector Types:Mammalian Expression, Adenoviral; Bacterial Resistance:Kanamycin
Defining Citation: PMID:16753578
Comments: pAdTrack can be recombined with pAdEasy to make adenoviral-PGC1.
See He et al. http://www.pnas.org/cgi/content/full/95/5/2509 for protocols.
Proper citation: RRID:Addgene_14426 Copy
Species: Mus musculus
Genetic Insert: Akt1 KD
Vector Backbone Description: Backbone Marker:BD Biosciences; Backbone Size:6500; Vector Backbone:pMSCV neo; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16365168
Proper citation: RRID:Addgene_14550 Copy
Species: Mus musculus
Genetic Insert: CCCNB2
Vector Backbone Description: Vector Backbone:pENTR_D-topo; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_136341 Copy
Species: Mus musculus
Genetic Insert: U6_sgRNA(Bcan)_U6_sgRNA(Ntrk1)
Vector Backbone Description: Backbone Marker:Addgene #50946; Vector Backbone:pKLV-U6gRNA-PGKpuro2ABFP; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29654229
Proper citation: RRID:Addgene_136413 Copy
Species: Mus musculus
Genetic Insert: src
Vector Backbone Description: Backbone Marker:BD Biosciences; Backbone Size:6600; Vector Backbone:pLNCX; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_13658 Copy
Species: Mus musculus
Genetic Insert: Sox2-T2A-Klf4
Vector Backbone Description: Backbone Size:6000; Vector Backbone:pHAGE2; Vector Types:Bacterial Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31708402
Proper citation: RRID:Addgene_136577 Copy
Species: Mus musculus
Genetic Insert: p53DD
Vector Backbone Description: Backbone Marker:Addgene #25752; Backbone Size:4422; Vector Backbone:pEN_TTmiRc2; Vector Types:Gateway Cloning; Bacterial Resistance:Gentamicin
Defining Citation: PMID:32291314
Proper citation: RRID:Addgene_136520 Copy
Species: Mus musculus
Genetic Insert: p53DD
Vector Backbone Description: Backbone Marker:Addgene #25736; Backbone Size:12944; Vector Backbone:pSLIK-Zeo; Vector Types:Mammalian Expression, Lentiviral, destinatioin vector for gateway cloning; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32291314
Proper citation: RRID:Addgene_136534 Copy
Species: Mus musculus
Genetic Insert: Oct4-P2A-Sox2-T2A-Klf4-E2A-cMyc
Vector Backbone Description: Backbone Size:6000; Vector Backbone:pHAGE2; Vector Types:Bacterial Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31708402
Proper citation: RRID:Addgene_136541 Copy
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