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http://www.addgene.org/61854

Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pEYFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Comments: Exactly the same as pEYFP-N1 but with AgeI-BsrGI swapped for venus fragment containing the point mutations F46L/F64L/V68L/M153T/V163A/S175G compared to EYFP

Proper citation: RRID:Addgene_61854 Copy   

•   Source: Addgene

http://www.addgene.org/61906

Genetic Insert: none
Vector Backbone Description: Vector Backbone:none; Vector Types:; Bacterial Resistance:Kanamycin
Defining Citation: PMID:21925267
Comments: Genotype = cyo::kan++ ilvE (Kan cassette in the chromosomal DNA) Precursor strain = CLY (Genotype cyo::kan) Selective amino acid labeling (and/or requirement) = Ile, Leu* CLY strain was derived from the C43(DE3)strain by transferring the cyo deletion (with kanamycin resistance cassette kanR) from an E. coli B strain to C43(DE3) strain by phage P1 [J. Biol. Chem. 282, 8777-8785 (2007)]. Thus, the kanR casette CANNOT be removed because there are NO FRT sites flanking kanR. In the presence of 0.4-1 mM Tyr, tyrB is repressed and Leu is required for growth in minimal medium. This strategy can only be applicable for a short-term cultivation but not suitable for a long-term cultivation for heterologous expression of foreign genes. Each target gene was deleted from the chromosome of C43 (DE3) E. coli strain using λ-Red recombination system. Supplemental documents contain a list of PCR primers used for verification of each knocked-out gene as well as an image showing PCR results for this strain.

Proper citation: RRID:Addgene_61906 Copy   

•   Source: Addgene

http://www.addgene.org/61907

Genetic Insert: none
Vector Backbone Description: Vector Backbone:none; Vector Types:; Bacterial Resistance:Kanamycin
Defining Citation: PMID:21925267
Comments: Genotype = cyo::kan hisG (Kan cassette in the chromosomal DNA) Precursor strain = CLY (Genotype cyo::kan) Selective amino acid labeling (and/or requirement) = His CLY strain was derived from the C43(DE3)strain by transferring the cyo deletion (with kanamycin resistance cassette kanR) from an E. coli B strain to C43(DE3) strain by phage P1 [J. Biol. Chem. 282, 8777-8785 (2007)]. Thus, the kanR casette CANNOT be removed because there are NO FRT sites flanking kanR. Each target gene was deleted from the chromosome of C43 (DE3) E. coli strain using λ-Red recombination system. Supplemental documents contain a list of PCR primers used for verification of each knocked-out gene as well as an image showing PCR results for this strain.

Proper citation: RRID:Addgene_61907 Copy   

•   Source: Addgene

http://www.addgene.org/61908

Genetic Insert: none
Vector Backbone Description: Vector Backbone:none; Vector Types:; Bacterial Resistance:Kanamycin
Defining Citation: PMID:21925267
Comments: Genotype = cyo::kan++ ilvE avtA (Kan cassette in the chromosomal DNA) Precursor strain = ML2 (Genotype cyo::kan) Selective amino acid labeling (and/or requirement) = Ile, Leu*, Val CLY strain was derived from the C43(DE3)strain by transferring the cyo deletion (with kanamycin resistance cassette kanR) from an E. coli B strain to C43(DE3) strain by phage P1 [J. Biol. Chem. 282, 8777-8785 (2007)]. Thus, the kanR casette CANNOT be removed because there are NO FRT sites flanking kanR. In the presence of 0.4-1 mM Tyr, tyrB is repressed and Leu is required for growth in minimal medium. This strategy can only be applicable for a short-term cultivation but not suitable for a long-term cultivation for heterologous expression of foreign genes. Each target gene was deleted from the chromosome of C43 (DE3) E. coli strain using λ-Red recombination system. Supplemental documents contain a list of PCR primers used for verification of each knocked-out gene as well as an image showing PCR results for this strain.

Proper citation: RRID:Addgene_61908 Copy   

•   Source: Addgene

http://www.addgene.org/61909

Genetic Insert: none
Vector Backbone Description: Vector Backbone:none; Vector Types:; Bacterial Resistance:Kanamycin
Defining Citation: PMID:21925267
Comments: Genotype = cyo::kan argH (Kan cassette in the chromosomal DNA) Precursor strain = CLY (Genotype cyo::kan) Selective amino acid labeling (and/or requirement) = Arg CLY strain was derived from the C43(DE3)strain by transferring the cyo deletion (with kanamycin resistance cassette kanR) from an E. coli B strain to C43(DE3) strain by phage P1 [J. Biol. Chem. 282, 8777-8785 (2007)]. Thus, the kanR casette CANNOT be removed because there are NO FRT sites flanking kanR. Each target gene was deleted from the chromosome of C43 (DE3) E. coli strain using λ-Red recombination system. Supplemental documents contain a list of PCR primers used for verification of each knocked-out gene as well as an image showing PCR results for this strain.

Proper citation: RRID:Addgene_61909 Copy   

•   Source: Addgene

http://www.addgene.org/61910

Genetic Insert: none
Vector Backbone Description: Vector Backbone:none; Vector Types:; Bacterial Resistance:Kanamycin
Defining Citation: PMID:21925267
Comments: Genotype = cyo::kan ilvE avtA aspC (Kan cassette in the chromosomal DNA) Precursor strain = ML6 (Genotype cyo::kan) Selective amino acid labeling (and/or requirement) = (Ala#), Ile, Leu*, Tyr#, Val CLY strain was derived from the C43(DE3)strain by transferring the cyo deletion (with kanamycin resistance cassette kanR) from an E. coli B strain to C43(DE3) strain by phage P1 [J. Biol. Chem. 282, 8777-8785 (2007)]. Thus, the kanR casette CANNOT be removed because there are NO FRT sites flanking kanR. *In the presence of 0.4-1 mM Tyr, tyrB is repressed and Leu is required for growth in minimal medium. This strategy can only be applicable for a short-term cultivation but not suitable for a long-term cultivation for heterologous expression of foreign genes [Methods 55, 370-378 (2011)]. #In the presence of 0.4-1 mM Tyr, tyrB is repressed and Tyr is required for growth in minimal medium. Under these conditions, this strain can also be used for selective labeling of the input Tyr and/or Ala label(s), although minor diffusion of the input label(s) can occur (see Tables 2, 3). Note that this strategy can only be applicable for a short-term cultivation but not suitable for a long-term cultivation for heterologous expression of foreign geness [Methods 55, 370-378 (2011)]. Each target gene was deleted from the chromosome of C43 (DE3) E. coli strain using λ-Red recombination system. Supplemental documents contain a list of PCR primers used for verification of each knocked-out gene as well as an image showing PCR results for this strain.

Proper citation: RRID:Addgene_61910 Copy   

•   Source: Addgene

http://www.addgene.org/61809

Species: Homo sapiens
Genetic Insert: UBL4A
Vector Backbone Description: Backbone Marker:Origene; Backbone Size:4929; Vector Backbone:pCMV6-ENTRY; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:24424410

Proper citation: RRID:Addgene_61809 Copy   

•   Source: Addgene

http://www.addgene.org/61813

Species: Thermoplasma volcanium
Genetic Insert: TvoVMAN11 intein
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:3559; Vector Backbone:pRSFDuet; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:24574026

Proper citation: RRID:Addgene_61813 Copy   

•   Source: Addgene

http://www.addgene.org/61817

Species: Pyrococcus horikoshii
Genetic Insert: PhoRadAdC6 intein
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:3559; Vector Backbone:pRSFDuet; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:24574026

Proper citation: RRID:Addgene_61817 Copy   

•   Source: Addgene

http://www.addgene.org/61839

Species: Homo sapiens
Genetic Insert: cyclin A
Vector Backbone Description: Backbone Marker:Pines Lab; Vector Backbone:pt7 venus cks1 (1843); Vector Types:in vitro transcription; Bacterial Resistance:Kanamycin
Defining Citation: PMID:20733051

Proper citation: RRID:Addgene_61839 Copy   

•   Source: Addgene

http://www.addgene.org/61972

Species: E. coli
Genetic Insert: lacZ alpha
Vector Backbone Description: Vector Backbone:pEGFP; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin

Proper citation: RRID:Addgene_61972 Copy   

•   Source: Addgene

http://www.addgene.org/62027

Species: Homo sapiens
Genetic Insert: Tmem194
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pEGFP-N2; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:20876400

Proper citation: RRID:Addgene_62027 Copy   

•   Source: Addgene

http://www.addgene.org/62023

Species: Homo sapiens
Genetic Insert: Tmem41A
Vector Backbone Description: Backbone Marker:unknown; Vector Backbone:pEGPF-N2; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:20693407

Proper citation: RRID:Addgene_62023 Copy   

•   Source: Addgene

http://www.addgene.org/62031

Species: Mus musculus
Genetic Insert: KLHL31
Vector Backbone Description: Backbone Marker:unknown; Vector Backbone:pgkRFP; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:20876400

Proper citation: RRID:Addgene_62031 Copy   

•   Source: Addgene

http://www.addgene.org/61995

Species: Homo sapiens
Genetic Insert: NET92
Vector Backbone Description: Backbone Marker:unknown; Vector Backbone:pmRFP-N2; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin

Proper citation: RRID:Addgene_61995 Copy   

•   Source: Addgene

http://www.addgene.org/61945

Species: Homo sapiens
Genetic Insert: MORC3:JMC01B-H03:C203041
Vector Backbone Description: Backbone Marker:Addgene Plasmid 26096; Vector Backbone:pET28-MHL; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: http://www.thesgc.org/structures/4QQ4/

Proper citation: RRID:Addgene_61945 Copy   

•   Source: Addgene

http://www.addgene.org/61946

Species: Homo sapiens
Genetic Insert: SMN1:JMC047-F11:C221089
Vector Backbone Description: Backbone Marker:Addgene Plasmid 26096; Vector Backbone:pET28-MHL; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: http://www.thesgc.org/structures/4QQ6/

Proper citation: RRID:Addgene_61946 Copy   

•   Source: Addgene

http://www.addgene.org/61948

Species: Homo sapiens
Genetic Insert: HTATIP:JMC001-B06:C41570
Vector Backbone Description: Backbone Marker:Addgene Plasmid 26096; Vector Backbone:pET28-MHL; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: http://www.thesgc.org/structures/4QQG/

Proper citation: RRID:Addgene_61948 Copy   

•   Source: Addgene

http://www.addgene.org/61949

Species: Homo sapiens
Genetic Insert: ANKRA:JMC023-D07:C211743
Vector Backbone Description: Backbone Marker:Addgene Plasmid 26096; Vector Backbone:pET28-MHL; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: http://www.thesgc.org/structures/4QQI/

Proper citation: RRID:Addgene_61949 Copy   

•   Source: Addgene

http://www.addgene.org/61954

Species: Homo sapiens
Genetic Insert: MLH1:JMC01L-F09:C205690
Vector Backbone Description: Backbone Marker:Addgene Plasmid 26096; Vector Backbone:pET28a-MHL; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: http://www.thesgc.org/structures/4P7A/

Proper citation: RRID:Addgene_61954 Copy   

•   Source: Addgene