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Species: Synthetic
Genetic Insert: PP4
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression, RNA transcription; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22492509
Proper citation: RRID:Addgene_83329 Copy
Species: Synthetic
Genetic Insert: PP3
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression, RNA transcription; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22492509
Proper citation: RRID:Addgene_83328 Copy
Species: Homo sapiens
Genetic Insert: human DNA-PKcs
Vector Backbone Description: Backbone Size:4665; Vector Backbone:pcmv6; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16314509
Proper citation: RRID:Addgene_83317 Copy
Vector Backbone Description: Backbone Marker:Rubin Lab; Backbone Size:11386; Vector Backbone:pBPGw; Vector Types:Insect Expression, Luciferase; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:28245922
Comments: VanGlow-GL-promoter is a Gateway compatible vector enabling high throughput cloning of promoter elements upstream of a GFP::Luciferase(nls) reporter protein (codon optimized for expression in Drosophila) using Invitrogen LR clonase. This vector can be used for Luciferase assays in S2 cells and can be inserted into specific target sites in the Drosophila genome using PhiC31 integrase and used to assess reporter gene expression in vivo using Immunofluorescence, Live-Cell-Imaging, and Luciferase assays. In addition, this vector may be used in combination with other VanGlow transgenes to facilitate FACS purification of specific populations of cells.
Proper citation: RRID:Addgene_83342 Copy
Species: Synthetic
Genetic Insert: PP10
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression, RNA transcription; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22492509
Proper citation: RRID:Addgene_83335 Copy
Species: Homo sapiens
Genetic Insert: XIAP
Vector Backbone Description: Backbone Marker:amersham; Backbone Size:4900; Vector Backbone:pGEX; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10797013
Proper citation: RRID:Addgene_8340 Copy
Species: Synthetic
Genetic Insert: PP6
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression, RNA transcription; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22492509
Proper citation: RRID:Addgene_83331 Copy
Genetic Insert: PAK1-AID-ires-EGFP
Vector Backbone Description: Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27321921
Proper citation: RRID:Addgene_83489 Copy
Species: Leptotrichia buccalis
Genetic Insert: Lbu_C2c2_WT
Vector Backbone Description: Backbone Size:5960; Vector Backbone:p2CT; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27669025
Comments: Codon optimized for E. Coli expression. His-MBP fusion tag can be removed with TEV protease.
Proper citation: RRID:Addgene_83482 Copy
Species: Listeria seeligeri
Genetic Insert: Lse_C2c2_WT
Vector Backbone Description: Backbone Size:5960; Vector Backbone:p2CT; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27669025
Comments: Codon optimized for E. Coli expression. His-MBP fusion tag can be removed with TEV protease.
Proper citation: RRID:Addgene_83486 Copy
Species: Homo sapiens
Genetic Insert: DLD
Vector Backbone Description: Backbone Marker:PMID: 26186194; Backbone Size:10056; Vector Backbone:pPHAGE C-TAP; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29128334
Comments: Mutation (first described in PMID: 17404228) was created using the method described in PMID: 17446874. Two outer (F: CTCTTGGTTTGCATTGGCCG, IRES_R: CCTCACATTGCCAAAAGACG) and two mutation-site primers were used. The mutated PCR product and the backbone vector were digested using EcoRI, gel purified and ligated.
Note that the mutation is numbered in accordance with the original publication (PMID: 17404228), which lacked the mitochondrial targeting signal (residues 1-35). In this full-length protein, it is found at position 491.
Proper citation: RRID:Addgene_83479 Copy
Species: Gallus gallus
Genetic Insert: FRNK
Vector Backbone Description: Backbone Marker:GE-Healthcare; Backbone Size:12769; Vector Backbone:TRIPZ-non-silencing shRNA control; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26216901
Proper citation: RRID:Addgene_83472 Copy
Species: Homo sapiens
Genetic Insert: SOD1
Vector Backbone Description: Backbone Marker:David Root; Backbone Size:10065; Vector Backbone:pLEX_307 (plasmid #41392); Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29128334
Comments: PCR cloning (F: TAAGCAGCTAGCAGCTCAGATCTCGAGCTCAAGC, R: TAAGCAACTAGTTCATTGGGCGATCCCAATTACACC) followed by cleanup, NheI/SpeI restriction digestion of PCR product and destination vector, gel purification and ligation.
Proper citation: RRID:Addgene_83446 Copy
Species: Mus musculus
Genetic Insert: CALR
Vector Backbone Description: Vector Backbone:pmcsg7; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19959473
Proper citation: RRID:Addgene_83505 Copy
Species: Synthetic
Genetic Insert: Barcode23
Vector Backbone Description: Vector Backbone:MSCV; Vector Types:Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27617390
Proper citation: RRID:Addgene_83700 Copy
Species: Synthetic
Genetic Insert: Barcode30
Vector Backbone Description: Vector Backbone:MSCV; Vector Types:Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27617390
Proper citation: RRID:Addgene_83707 Copy
Species: Synthetic
Genetic Insert: Barcode32
Vector Backbone Description: Vector Backbone:MSCV; Vector Types:Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27617390
Proper citation: RRID:Addgene_83709 Copy
Species: Synthetic
Genetic Insert: Barcode8
Vector Backbone Description: Vector Backbone:MSCV; Vector Types:Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27617390
Proper citation: RRID:Addgene_83685 Copy
Species: Synthetic
Genetic Insert: Barcode11
Vector Backbone Description: Vector Backbone:MSCV; Vector Types:Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27617390
Proper citation: RRID:Addgene_83688 Copy
Species: Synthetic
Genetic Insert: Barcode44
Vector Backbone Description: Vector Backbone:MSCV; Vector Types:Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27617390
Proper citation: RRID:Addgene_83721 Copy
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