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Species: Homo sapiens
Genetic Insert: MLCK
Vector Backbone Description: Backbone Marker:Iain Fraser (Addgene plasmid # 25734); Vector Backbone:pSLIK; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25634210
Comments: The MLCK insert starts at amino acid 885 and contains D899E, D914A, N1782T, K1785R, and S1788T amino acid mutations compared to best match reference sequence (NP_444253.3).
Proper citation: RRID:Addgene_84647 Copy
Genetic Insert: mCyRFP1
Vector Backbone Description: Backbone Size:2834; Vector Backbone:pNCS; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27798609
Proper citation: RRID:Addgene_84545 Copy
Vector Backbone Description: Backbone Size:0; Vector Backbone:na; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12649500
Comments: Packaging plasmid for Lentiviral constructs (vpr is removed). The 293T cell line can be obtained from the Weinberg lab or GenHunter http://genhunter.com/products/aptag-3/index.html
*Addgene Comment: This plasmid contains some HIV accessory genes. Please reach out to your biosafety officer for guidance. The definition of "2nd generation" for this plasmid differs from the standard definition.
Proper citation: RRID:Addgene_8455 Copy
Species: Acidaminococcus_sp_BV3L6
Genetic Insert: AsCpf1
Vector Backbone Description: Backbone Size:8700; Vector Backbone:lentiCas9-Blast(Addgene#:52962); Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27992409
Proper citation: RRID:Addgene_84750 Copy
Species: Synthetic
Genetic Insert: huLbCpf1
Vector Backbone Description: Backbone Size:8069; Vector Backbone:pHKO_23; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27918548
Comments: Contains a BsmBI cloning cassette for insertion of Cpf1 guides under U6 promoter control.
Proper citation: RRID:Addgene_84740 Copy
Species: Homo sapiens
Genetic Insert: Ngn2 and Mash1 with a T2A
Vector Backbone Description: Backbone Marker:clonetech; Backbone Size:7791; Vector Backbone:PLVX TIGHT PURO; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26456686
Proper citation: RRID:Addgene_84777 Copy
Vector Backbone Description: Backbone Marker:Luigi Naldini , PMID 15619618; Vector Backbone:BdLV; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24633241
Proper citation: RRID:Addgene_84771 Copy
Species: Other
Genetic Insert: LFn
Vector Backbone Description: Backbone Marker:Novagen; Vector Backbone:pET15b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27045008
Proper citation: RRID:Addgene_84872 Copy
Species: Synthetic
Genetic Insert: PA50
Vector Backbone Description: Backbone Marker:Susan Lindquist (Addgene plasmid # 14147); Backbone Size:5647; Vector Backbone:pAG416-Gal-ccdB; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26308983
Proper citation: RRID:Addgene_84902 Copy
Species: Synthetic
Genetic Insert: PA50
Vector Backbone Description: Backbone Marker:Susan Lindquist (Addgene plasmid # 14133); Backbone Size:5192; Vector Backbone:pAG303-Gal-ccdB; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26308983
Proper citation: RRID:Addgene_84906 Copy
Species: Synthetic
Genetic Insert: PR50
Vector Backbone Description: Backbone Marker:Susan Lindquist (Addgene plasmid # 14133); Backbone Size:5192; Vector Backbone:pAG303-Gal-ccdB; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26308983
Proper citation: RRID:Addgene_84905 Copy
Species: Synthetic
Genetic Insert: GA50
Vector Backbone Description: Backbone Marker:Susan Lindquist (Addgene plasmid # 14133); Backbone Size:5192; Vector Backbone:pAG303-Gal-ccdB; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26308983
Proper citation: RRID:Addgene_84907 Copy
Species: Homo sapiens
Genetic Insert: Gli3
Vector Backbone Description: Backbone Size:6300; Vector Backbone:C-Terminal p3xFLAG-CMV14; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_84921 Copy
Species: Other
Genetic Insert: Sico-Red
Vector Backbone Description: Backbone Marker:stratagene; Backbone Size:3417; Vector Backbone:pAAV-MCS; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27358580
Comments: Addgene QC NGS identified that this plasmid includes the Best1 targeted sequence downstream of GFP. See the associated publication (PMID: 27358580) for additional information.
Proper citation: RRID:Addgene_84882 Copy
Species: Caenorhabditis elegans
Genetic Insert: C. elegans codon optimized tdTomato
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pDESTR4-R3; Vector Types:Bacterial Expression, Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27374334
Comments: Expression vector with C. elegans codon-optimized tdTomato under the eft-3 promoter and with the tbb-2 3'UTR.
Cloned into pCFJ150 for compatibility with mosSCI.
Contains cbr-unc-119 rescue fragment.
Proper citation: RRID:Addgene_84830 Copy
Genetic Insert: mCherry
Vector Backbone Description: Backbone Size:9077; Vector Backbone:pMMB207c; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:28257493
Proper citation: RRID:Addgene_84821 Copy
Species: Entacmaea quadricolor, Picorna virus, E coli, HSV
Genetic Insert: RFP-P2A-tTA
Vector Backbone Description: Backbone Size:5590; Vector Backbone:pAAV-Ef1a-DIO EYFP (Plasmid #27056); Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27775045
Proper citation: RRID:Addgene_85039 Copy
Species: HPV 197
Genetic Insert: HPV 197 E7
Vector Backbone Description: Backbone Size:6600; Vector Backbone:CMV bam neo; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27771561
Proper citation: RRID:Addgene_85034 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: NLS-FLPe-WPRE
Vector Backbone Description: Backbone Size:2581; Vector Backbone:pTRE-tight; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27775045
Proper citation: RRID:Addgene_85007 Copy
Species: Homo sapiens
Genetic Insert: UbcH10
Vector Backbone Description: Backbone Size:2488; Vector Backbone:pT7-7; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9122200
Comments: See author's map. (Ruderman pHSC6.11)
Proper citation: RRID:Addgene_8505 Copy
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