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http://www.addgene.org/170605

Species: Homo sapiens
Genetic Insert: miniTurbo-V5-RPS6KL1
Vector Backbone Description: Backbone Marker:PEL; Backbone Size:9019; Vector Backbone:pDest663; Vector Types:Mammalian Expression, Lentiviral, Gateway Destination; Bacterial Resistance:Ampicillin
Comments: These plasmids were generated as part of the Illuminating the Druggable Genome (IDG) program sponsored by the NIH Common Fund. The goal of this program is to identify, gather, and distribute information and resources for proteins that currently are not well-studied yet belong to commonly drug-targeted protein families: protein kinases, non-olfactory G-protein coupled receptors (GPCRs), and ion channels. The IDG program is designed to develop fundamental research tools for understudied proteins, elucidate their function, and disseminate the IDG-related resources and data to the greater scientific community. These lentiviral gateway destination plasmids were generated, as part of the Kinase-Data and Resource Generating Center (DRGC), using the single fragment or multisite gateway cloning technology. They were used for generating proteomics-based protein-interaction and protein-proximity networks that can be accessed through the kinase-DRGC website https://darkkinome.org/. Each plasmid has either an N- or C-terminal fusion protein (Flag/ V5/V5-miniTurbo/V5-TurboID/miniTurbo-V5/TurboID-V5) tagged understudied kinase driven under either a CMV or Ubiquitin (UBC) promoter. All inserts in the pENTR plasmids used to generate the deposited destination plasmids were end-sequenced and insert size validated prior to multi-site gateway cloning. The combined insert size of the single fragment or three fragment destination plasmids were confirmed by restriction digestion (BsrGI, and EcoRV for pDest667; BsrGI, EcoRV, and BstZ17I for pDest663; and BsrGI for pHAGE) and all plasmids were partially sequenced to ensure in-frame ORFs and fusion tags.

Proper citation: RRID:Addgene_170605 Copy   

•   Source: Addgene

http://www.addgene.org/170756

Vector Backbone Description: Backbone Size:5409; Vector Backbone:pJCX4; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34478714
Comments: Please visit https://www.biorxiv.org/content/10.1101/2021.04.05.438513v2 for BioRxiv preprint

Proper citation: RRID:Addgene_170756 Copy   

•   Source: Addgene

http://www.addgene.org/170636

Species: Vibrio cholera
Genetic Insert: VchTniQ, VchCas8, VchCas7, VchCas6, CRISPR(8+IS186 array)
Vector Backbone Description: Vector Backbone:pUC19; Vector Types:Bacterial Expression; Bacterial Resistance:Streptomycin
Defining Citation: PMID:34152213

Proper citation: RRID:Addgene_170636 Copy   

•   Source: Addgene

http://www.addgene.org/170633

Species: Vibrio cholera
Genetic Insert: VchTniQ, VchCas8, VchCas7, VchCas6, CRISPR(BsaI)
Vector Backbone Description: Vector Backbone:pCDFDuet-1; Vector Types:Bacterial Expression; Bacterial Resistance:Streptomycin
Defining Citation: PMID:34152213

Proper citation: RRID:Addgene_170633 Copy   

•   Source: Addgene

http://www.addgene.org/170631

Species: Vibrio cholera
Genetic Insert: VchTniQ, VchCas8, VchCas7, VchCas6, CRISPR(BsaI)
Vector Backbone Description: Vector Backbone:pCDFDuet-1; Vector Types:Bacterial Expression; Bacterial Resistance:Streptomycin
Defining Citation: PMID:34152213

Proper citation: RRID:Addgene_170631 Copy   

•   Source: Addgene

http://www.addgene.org/170753

Species: Gallus gallus
Genetic Insert: PLA2G4A-C2 domain
Vector Backbone Description: Vector Backbone:pSUMO; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31050338

Proper citation: RRID:Addgene_170753 Copy   

•   Source: Addgene

http://www.addgene.org/170637

Species: Saccharomyces cerevisiae
Genetic Insert: Mini-Tn6677, GNAc cassette, lacI
Vector Backbone Description: Vector Backbone:pUC57mini; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34152213

Proper citation: RRID:Addgene_170637 Copy   

•   Source: Addgene

http://www.addgene.org/170625

Species: Synthetic
Genetic Insert: Lyn11-myc-LOV2GIVe (D)
Vector Backbone Description: Backbone Size:6000; Vector Backbone:pLIC-myc; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32936073

Proper citation: RRID:Addgene_170625 Copy   

•   Source: Addgene

http://www.addgene.org/170629

Species: Vibrio cholera
Genetic Insert: VchTniQ, VchCas8, VchCas7, VchCas6, CRISPR(BsaI)
Vector Backbone Description: Vector Backbone:pCDFDuet-1; Vector Types:Bacterial Expression; Bacterial Resistance:Streptomycin
Defining Citation: PMID:34152213

Proper citation: RRID:Addgene_170629 Copy   

•   Source: Addgene

http://www.addgene.org/17072

Species: Danio rerio
Genetic Insert: Prickle-1
Vector Backbone Description: Backbone Size:4100; Vector Backbone:CS2P+; Vector Types:Zebrafish Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12699626
Comments: Description: Zebrafish prickle-1 (full-length ORF), cloned into the BamH1 and EcoR1 sites of CS2P+. Species of Sequence Origin: zebrafish. Notes for Use: Has internal Not1, Kp1 sites, so linearize with BssH11 and transcribe SP6 for sense RNA. Select clones with amp. Reference: MT Veeman et al., Current Biology 13: 680-685 (2003).

Proper citation: RRID:Addgene_17072 Copy   

•   Source: Addgene

http://www.addgene.org/17064

Species: Danio rerio
Genetic Insert: vox-2
Vector Backbone Description: Backbone Size:4100; Vector Backbone:pCS2+; Vector Types:Zebrafish Expression; Bacterial Resistance:Ampicillin
Comments: Zvox-2 coding region amplified by PCR and inserted into CS2+ (first cloned into SK as zV93). For RNA: cut with Asp718 and transcribe with SP6. Plasmid History: Species of Sequence Origin: Zebrafish. Constructed by: David Kimelman, 6/28/97 XIX p. 67.

Proper citation: RRID:Addgene_17064 Copy   

•   Source: Addgene

http://www.addgene.org/17068

Species: Danio rerio
Genetic Insert: Stbm
Vector Backbone Description: Backbone Size:4100; Vector Backbone:pCS2+; Vector Types:Zebrafish Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11780127
Comments: 6-c-myc epitopes in frame at the amino terminus of wild type zebrafish strabismus; EcoRI-Xba1 insert. For Sense RNA: linearize with ASP 718 and transcribe with SP6 polymerase. Plasmid History: Species of Sequence Origin: Zebrafish. Reference: M. Park and R. Moon, Nature Cell Biology 4, 20-25, 2002.

Proper citation: RRID:Addgene_17068 Copy   

•   Source: Addgene

http://www.addgene.org/170810

Vector Backbone Description: Vector Backbone:lentiCRISPR v2 (Addgene #52961); Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34133940
Comments: 3rd generation lentiviral backbone. Please visit https://www.biorxiv.org/content/10.1101/2020.06.24.165795v2.full for bioRxiv preprint.

Proper citation: RRID:Addgene_170810 Copy   

•   Source: Addgene

http://www.addgene.org/170774

Species: Homo sapiens
Genetic Insert: LAMP1
Vector Backbone Description: Backbone Marker:Addgene (Plasmid #14883); Backbone Size:9955; Vector Backbone:FUGW; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34102054
Comments: Chin et al. ACS Sens. 2021 Jun 8. doi: 10.1021/acssensors.0c02318. Pubmed: https://pubmed.ncbi.nlm.nih.gov/34102054/ Article: https://pubs.acs.org/doi/10.1021/acssensors.0c02318

Proper citation: RRID:Addgene_170774 Copy   

•   Source: Addgene

http://www.addgene.org/170775

Species: Homo sapiens
Genetic Insert: LAMP1
Vector Backbone Description: Backbone Marker:Addgene Plasmid #19319; Backbone Size:8083; Vector Backbone:pLJM1-EGFP; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34102054
Comments: Chin et al. ACS Sens. 2021 Jun 8. doi: 10.1021/acssensors.0c02318. Pubmed: https://pubmed.ncbi.nlm.nih.gov/34102054/ Article: https://pubs.acs.org/doi/10.1021/acssensors.0c02318

Proper citation: RRID:Addgene_170775 Copy   

•   Source: Addgene

http://www.addgene.org/170772

Species: Synthetic
Genetic Insert: lacI
Vector Backbone Description: Backbone Marker:pTwist; Backbone Size:3; Vector Backbone:pUC; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35255734
Comments: This plasmid was designed to test Golden Gate assembly protocols

Proper citation: RRID:Addgene_170772 Copy   

•   Source: Addgene

http://www.addgene.org/170818

Species: Homo sapiens
Genetic Insert: PCNA Homology Arms with mEGFP-linker
Vector Backbone Description: Vector Backbone:pUC57; Vector Types:Mammalian Expression, CRISPR, Donor Template; Bacterial Resistance:Ampicillin
Comments: This plasmid has been used with locus-specific CRISPR/Cas9 to add a mEGFP tag to the N-terminus of human PCNA in WTC-11 human induced pluripotent stem cells by the Allen Institute for Cell Science. Linker (AA) sequence: GEGQGQGQGPGRGYAYRS. After protein tagging using this donor template plasmid and CRISPR/Cas9 reagents, transfected cells may exhibit varying intensity levels of fluorescence, likely due to editing precision. To obtain cells of uniform intensity levels, see our protocol for fluorescence-assisted cell sorting and subcloning of transfected cells (https://www.allencell.org/instructional-videos-and-tutorials-for-cell-methods.html). Further, we recommend PCR-based assays for identifying precisely edited clones as previously described (https://www.molbiolcell.org/doi/abs/10.1091/mbc.e17-03-0209). For more information on the entire plasmid collection, please see https://www.addgene.org/allen-institute-cell-science/ .

Proper citation: RRID:Addgene_170818 Copy   

•   Source: Addgene

http://www.addgene.org/170813

Species: Synthetic; B. coriaceae
Genetic Insert: NLS-mScarlet-I-P2A-BlastR
Vector Backbone Description: Vector Backbone:pW212 (Addgene #170810); Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34133940
Comments: 3rd generation lentiviral backbone. Please visit https://www.biorxiv.org/content/10.1101/2020.06.24.165795v2.full for bioRxiv preprint.

Proper citation: RRID:Addgene_170813 Copy   

•   Source: Addgene

http://www.addgene.org/170770

Species: Synthetic
Genetic Insert: lacI
Vector Backbone Description: Backbone Marker:GenScript; Backbone Size:1835; Vector Backbone:pUC; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35255734
Comments: This plasmid was designed to test 5RM assembly protocols

Proper citation: RRID:Addgene_170770 Copy   

•   Source: Addgene

http://www.addgene.org/170771

Species: Synthetic
Genetic Insert: tagRFP
Vector Backbone Description: Backbone Marker:GenScript; Backbone Size:2579; Vector Backbone:pUC; Vector Types:; Bacterial Resistance:Kanamycin
Defining Citation: PMID:35255734
Comments: This plasmid was designed to test Golden Gate assembly protocols

Proper citation: RRID:Addgene_170771 Copy   

•   Source: Addgene