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Species: Synthetic
Genetic Insert: PLEKHG4
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31469028
Proper citation: RRID:Addgene_129618 Copy
Species: Synthetic
Genetic Insert: PREX1
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31469028
Proper citation: RRID:Addgene_129619 Copy
Species: Synthetic
Genetic Insert: FGD5
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31469028
Proper citation: RRID:Addgene_129613 Copy
Species: Synthetic
Genetic Insert: ITSN1
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31469028
Proper citation: RRID:Addgene_129614 Copy
Species: Synthetic
Genetic Insert: ITSN2
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31469028
Comments: Please note: Plasmid contains N724D, M1499V, and I1857V mutations in the mCherry-ITSN2 insert. These mutations are not known to affect plasmid function.
Proper citation: RRID:Addgene_129615 Copy
Species: Synthetic
Genetic Insert: PLEKHG1
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31469028
Comments: Please note: Plasmid contains a single A nucleotide deletion at amino acid K1574 in PLEKHG1 that causes frameshift and early termination. This is not known to affect plasmid function.
Proper citation: RRID:Addgene_129616 Copy
Species: Synthetic
Genetic Insert: F-tractin
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_129606 Copy
Species: Synthetic
Genetic Insert: Alpha-PIX
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31469028
Proper citation: RRID:Addgene_129609 Copy
Species: Synthetic
Genetic Insert: Rab7
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_129603 Copy
Species: Synthetic
Genetic Insert: Paxillin
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Comments: Please note: Plasmid contains A9E and A87S mutations in NeoR/KanR. These mutations are not known to affect plasmid function.
Proper citation: RRID:Addgene_129604 Copy
Species: Synthetic
Genetic Insert: C1(1-29)-APEX2-GFP
Vector Backbone Description: Vector Backbone:pcDNA5/FRT/To; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31442426
Proper citation: RRID:Addgene_129641 Copy
Species: Synthetic
Genetic Insert: H2A-mScarletI-P2A-Akt-KTR-mTurquoise2-P2A-ERK-KTR-mNeonGreen
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:35107584
Comments: Please visit https://www.biorxiv.org/content/10.1101/2021.07.27.453948v3 for bioRxiv preprint.
Proper citation: RRID:Addgene_129631 Copy
Species: Synthetic
Genetic Insert: Myristoylation sequence (MGRRKRKPK-)
Vector Backbone Description: Backbone Size:2682; Vector Backbone:pGGC000; Vector Types:GreenGate Entry plasmid; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31844834
Comments: Please visit https://www.biorxiv.org/content/10.1101/734616v2 for bioRxiv preprint.
Proper citation: RRID:Addgene_129735 Copy
Species: Synthetic
Genetic Insert: cis-aconitate decarboxylase
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:3519; Vector Backbone:pCOLADuet-1; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31548418
Proper citation: RRID:Addgene_124842 Copy
Species: Synthetic
Genetic Insert: SPOON
Vector Backbone Description: Backbone Size:5400; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29963852
Proper citation: RRID:Addgene_124840 Copy
Species: Synthetic
Genetic Insert: PyronicSF
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:3097; Vector Backbone:pBI-CMV1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32142409
Comments: Please visit https://www.biorxiv.org/content/10.1101/611806v1 for BioRxiv preprint
Proper citation: RRID:Addgene_124830 Copy
Species: Synthetic
Genetic Insert: mTagBFP2-T2A-FLPo
Vector Backbone Description: Vector Backbone:pCAGGS; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: In the NGS sequence, polymorphisms are present in a GC-rich region of the Chicken beta-actin promoter that do not affect the function of the plasmid.
Proper citation: RRID:Addgene_124834 Copy
Species: Synthetic
Genetic Insert: pac-T2A-FLPo
Vector Backbone Description: Vector Backbone:pCAGGS; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_124835 Copy
Species: Synthetic
Genetic Insert: pac-T2A-dre
Vector Backbone Description: Vector Backbone:pCAGGS; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_124833 Copy
Species: Synthetic
Genetic Insert: Cas9
Vector Backbone Description: Backbone Size:3000; Vector Backbone:pMCSV; Vector Types:Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26969085
Comments: Guide RNA to be cloned into BbsI site. Supplied modified golden-gate cloning method is required given that extra BbsI site is located within the 2A sequence.
To design guide RNAs:
-add ACCG to 5' end of positive strand (positive strand defined as strand which will contact PAM sequence at 3' end)
-add AAAC to 5' end of negative strand
Proper citation: RRID:Addgene_124889 Copy
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